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01-06-2010 | Research Paper

Genomics-based screening of differentially expressed genes in the brains of mice exposed to silver nanoparticles via inhalation

Authors: Hye-Young Lee, You-Jin Choi, Eun-Jung Jung, Hu-Quan Yin, Jung-Taek Kwon, Ji-Eun Kim, Hwang-Tae Im, Myung-Haing Cho, Ju-Han Kim, Hyun-Young Kim, Byung-Hoon Lee

Published in: Journal of Nanoparticle Research | Issue 5/2010

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Abstract

Silver nanoparticles (AgNP) are among the fastest growing product categories in the nanotechnology industry. Despite the importance of AgNP in consumer products and clinical applications, relatively little is known regarding AgNP toxicity and its associated risks. We investigated the effects of AgNP on gene expression in the mouse brain using Affymetrix Mouse Genome Arrays. C57BL/6 mice were exposed to AgNP (geometric mean diameter, 22.18 ± 1.72 nm; 1.91 × 10⁷ particles/cm³) for 6 h/day, 5 days/week using the nose-only exposure system for 2 weeks. Total RNA isolated from the cerebrum and cerebellum was subjected to hybridization. From over 39,000 probe sets, 468 genes in the cerebrum and 952 genes in the cerebellum were identified as AgNP-responsive (one-way analysis of variance; p < 0.05). The largest groups of gene products affected by AgNP exposure included 73 genes in the cerebrum and 144 genes in the cerebellum. AgNP exposure modulated the expression of several genes associated with motor neuron disorders, neurodegenerative disease, and immune cell function, indicating potential neurotoxicity and immunotoxicity associated with AgNP exposure. Real-time PCR data for five genes analyzed from whole blood showed good correlation with the observed changes in the brain. Following rigorous validation and substantiation, these genes may assist in the development of surrogate markers for AgNP exposure and/or toxicity.

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Title: Genomics-based screening of differentially expressed genes in the brains of mice exposed to silver nanoparticles via inhalation
Authors: Hye-Young Lee
You-Jin Choi
Eun-Jung Jung
Hu-Quan Yin
Jung-Taek Kwon
Ji-Eun Kim
Hwang-Tae Im
Myung-Haing Cho
Ju-Han Kim
Hyun-Young Kim
Byung-Hoon Lee
Publication date: 01-06-2010
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 5/2010
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-009-9666-2

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