Top

Journal of Nanoparticle Research

Published in:

01-01-2010 | Research Paper

Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages

Authors: Chen-Hao Yeh, Jong-Kai Hsiao, Jaw-Lin Wang, Fuu Sheu

Published in: Journal of Nanoparticle Research | Issue 1/2010

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Ferucarbotran, a clinically used superparamagnetic iron oxide, is widely developed as a magnetic resonance imaging (MRI) contrast agent and has the potential to improve the monitoring of macrophage recirculation in vivo. However, the biological effect of Ferucarbotran or magnetic nanoparticles (MNPs) on macrophage is not clearly understood yet. This study is aimed to examine the immunological impact of Ferucarbotran toward murine peritoneal macrophages. Cells treated with Ferucarbotran demonstrated a dose–responsive increase of granularity in the cytoplasm. After 24 h of incubation, viability and cytotoxicity in macrophages treated with 200 μg Fe/mL of Ferucarbotran were not affected. Macrophages loaded with Ferucarbotran above 100 μg Fe/mL showed a significant (p < 0.01) increase in cytokine (TNF-α, IL-1β, IL-6) secretion and mRNA expression, followed by nitric oxide (NO) secretion and iNOS mRNA expression. Chemotactic responses of Ferucarbotran-preloaded macrophages toward CX3CL1 were significantly (p < 0.05) lower than those of untreated macrophages. Taking together, Ferucarbotran at high dose (100 μg Fe/mL) could induce murine peritoneal macrophages activation in pro-inflammatory cytokine secretion and NO production.

previous article Porous ZnO nanobelts: synthesis, mechanism, and morphological evolutions

next article Molten salt synthesis of LaF3:Eu3+ nanoplates with tunable size and their luminescence properties

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Available only for authorised users

Arbab AS, Bashaw LA, Miller BR et al (2003) Characterization of biophysical and metabolic properties of cells labeled with superparamagnetic iron oxide nanoparticles and transfection agent for cellular MR imaging. Radiology 229:838–846. doi:10.1148/radiol.2293021215 CrossRefPubMed

Arbab AS, Yocum GT, Kalish H et al (2004) Efficient magnetic cell labeling with protamine sulfate complexed to ferumoxides for cellular MRI. Blood 104:1217–1223. doi:10.1182/blood-2004-02-0655 CrossRefPubMed

Arbab AS, Yocum GT, Rad AM et al (2005) Labeling of cells with ferumoxides-protamine sulfate complexes does not inhibit function or differentiation capacity of hematopoietic or mesenchymal stem cells. NMR Biomed 18:553–559. doi:10.1002/nbm.991 CrossRefPubMed

Barlic J, Sechler JM, Murphy PM (2003) IL-15 and IL-2 oppositely regulate expression of the chemokine receptor CX3CR1. Blood 102:3494–3503. doi:10.1182/blood-2003-03-0946 CrossRefPubMed

Bazan JF, Bacon KB, Hardiman G et al (1997) A new class of membrane-bound chemokine with a CX3C motif. Nature 385:640–644. doi:10.1038/385640a0 CrossRefPubMedADS

Chen JJ, Lin YC, Yao PL et al (2005) Tumor-associated macrophages: the double-edged sword in cancer progression. J Clin Oncol 23:953–964. doi:10.1200/JCO.2005.12.172 CrossRefPubMed

Corot C, Petry KG, Trivedi R et al (2004) Macrophage imaging in central nervous system and in carotid atherosclerotic plaque using ultrasmall superparamagnetic iron oxide in magnetic resonance imaging. Invest Radiol 39:619–625. doi:10.1097/01.rli.0000135980.08491.33 CrossRefPubMed

Corot C, Robert P, Idee JM, Port M (2006) Recent advances in iron oxide nanocrystal technology for medical imaging. Adv Drug Deliv Rev 58:1471–1504. doi:10.1016/j.addr.2006.09.013 CrossRefPubMed

Daldrup-Link HE, Rudelius M, Oostendorp RA et al (2003) Targeting of hematopoietic progenitor cells with MR contrast agents. Radiology 228:760–767. doi:10.1148/radiol.2283020322 CrossRefPubMed

Dodd SJ, Williams M, Suhan JP, Williams DS, Koretsky AP, Ho C (1999) Detection of single mammalian cells by high-resolution magnetic resonance imaging. Biophys J 76:103–109CrossRefPubMed

Dousset V, Delalande C, Ballarino L et al (1999) In vivo macrophage activity imaging in the central nervous system detected by magnetic resonance. Magn Reson Med 41:329–333. doi:10.1002/(SICI)1522-2594(199902)41:2<329::AID-MRM17>3.0.CO;2-ZCrossRefPubMed

Floris S, Blezer EL, Schreibelt G et al (2004) Blood–brain barrier permeability and monocyte infiltration in experimental allergic encephalomyelitis: a quantitative MRI study. Brain 127:616–627. doi:10.1093/brain/awh068 CrossRefPubMed

Fotakis G, Timbrell JA (2006) In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 160:171–177. doi:10.1016/j.toxlet.2005.07.001 CrossRefPubMed

Gevrey JC, Isaac BM, Cox D (2005) Syk is required for monocyte/macrophage chemotaxis to CX3CL1 (Fractalkine). J Immunol 175:3737–3745PubMed

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR (1982) Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids. Anal Biochem 126:131–138. doi:10.1016/0003-2697(82)90118-X CrossRefPubMed

Hamm B, Staks T, Taupitz M (1994a) SHU 555A A new superparamagnetic iron oxide contrast agent for magnetic resonance imaging. Invest Radiol 29(Suppl 2):S87–S89. doi:10.1097/00004424-199406001-00029 PubMedCrossRef

Hamm B, Staks T, Taupitz M et al (1994b) Contrast-enhanced MR imaging of liver and spleen: first experience in humans with a new superparamagnetic iron oxide. J Magn Reson Imaging 4:659–668. doi:10.1002/jmri.1880040508 CrossRefPubMed

Hinds KA, Hill JM, Shapiro EM et al (2003) Highly efficient endosomal labeling of progenitor and stem cells with large magnetic particles allows magnetic resonance imaging of single cells. Blood 102:867–872. doi:10.1182/blood-2002-12-3669 CrossRefPubMed

Hsiao JK, Chu HH, Wang YH et al (2008) Macrophage physiological function after superparamagnetic iron oxide labeling. NMR Biomed 21:820–829. doi:10.1002/nbm.1260 CrossRefPubMed

Jendelova P, Herynek V, De Croos J et al (2003) Imaging the fate of implanted bone marrow stromal cells labeled with superparamagnetic nanoparticles. Magn Reson Med 50:767–776. doi:10.1002/mrm.10585 CrossRefPubMed

Kimball ES, Kovacs E, Clark MC, Schneider CR (1995) Activation of cytokine production and adhesion molecule expression on THP-1 myelomonocytic cells by macrophage colony-stimulating factor in combination with interferon-gamma. J Leukoc Biol 58:585–594PubMed

Krieg FM, Andres RY, Winterhalter KH (1995) Superparamagnetically labelled neutrophils as potential abscess-specific contrast agent for MRI. Magn Reson Imaging 13:393–400. doi:10.1016/0730-725X(94)00111-F CrossRefPubMed

Metz S, Bonaterra G, Rudelius M, Settles M, Rummeny EJ, Daldrup-Link HE (2004) Capacity of human monocytes to phagocytose approved iron oxide MR contrast agents in vitro. Eur Radiol 14:1851–1858. doi:10.1007/s00330-004-2405-2 CrossRefPubMed

Moghimi SM, Hunter AC, Murray JC (2005) Nanomedicine: current status and future prospects. FASEB J 19:311–330. doi:10.1096/fj.04-2747rev CrossRefPubMed

Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63. doi:10.1016/0022-1759(83)90303-4 CrossRefPubMed

Muller K, Skepper JN, Posfai M et al (2007) Effect of ultrasmall superparamagnetic iron oxide nanoparticles (Ferumoxtran-10) on human monocyte-macrophages in vitro. Biomaterials 28:1629–1642. doi:10.1016/j.biomaterials.2006.12.003 CrossRefPubMed

Muller K, Skepper JN, Tang TY et al (2008) Atorvastatin and uptake of ultrasmall superparamagnetic iron oxide nanoparticles (Ferumoxtran-10) in human monocyte-macrophages: implications for magnetic resonance imaging. Biomaterials 29:2656–2662. doi:10.1016/j.biomaterials.2008.03.006 CrossRefPubMed

Oca-Cossio J, Mao H, Khokhlova N et al (2004) Magnetically labeled insulin-secreting cells. Biochem Biophys Res Commun 319:569–575. doi:10.1016/j.bbrc.2004.04.195 CrossRefPubMed

Pan Y, Lloyd C, Zhou H et al (1997) Neurotactin, a membrane-anchored chemokine upregulated in brain inflammation. Nature 387:611–617. doi:10.1038/42491 CrossRefPubMedADS

Pawelczyk E, Arbab AS, Pandit S, Hu E, Frank JA (2006) Expression of transferrin receptor and ferritin following ferumoxides-protamine sulfate labeling of cells: implications for cellular magnetic resonance imaging. NMR Biomed 19:581–592. doi:10.1002/nbm.1038 CrossRefPubMed

Rausch M, Hiestand P, Baumann D, Cannet C, Rudin M (2003) MRI-based monitoring of inflammation and tissue damage in acute and chronic relapsing EAE. Magn Reson Med 50:309–314. doi:10.1002/mrm.10541 CrossRefPubMed

Raynal I, Prigent P, Peyramaure S, Najid A, Rebuzzi C, Corot C (2004) Macrophage endocytosis of superparamagnetic iron oxide nanoparticles: mechanisms and comparison of ferumoxides and ferumoxtran-10. Invest Radiol 39:56–63. doi:10.1097/01.rli.0000101027.57021.28 CrossRefPubMed

Reimer P, Balzer T (2003) Ferucarbotran (Resovist): a new clinically approved RES-specific contrast agent for contrast-enhanced MRI of the liver: properties, clinical development, and applications. Eur Radiol 13:1266–1276PubMed

Smirnov P, Lavergne E, Gazeau F et al (2006) In vivo cellular imaging of lymphocyte trafficking by MRI: a tumor model approach to cell-based anticancer therapy. Magn Reson Med 56:498–508. doi:10.1002/mrm.20996 CrossRefPubMed

Zhang Y, Dodd SJ, Hendrich KS, Williams M, Ho C (2000) Magnetic resonance imaging detection of rat renal transplant rejection by monitoring macrophage infiltration. Kidney Int 58:1300–1310. doi:10.1046/j.1523-1755.2000.00286.x CrossRefPubMed

Title: Immunological impact of magnetic nanoparticles (Ferucarbotran) on murine peritoneal macrophages
Authors: Chen-Hao Yeh
Jong-Kai Hsiao
Jaw-Lin Wang
Fuu Sheu
Publication date: 01-01-2010
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 1/2010
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-009-9589-y

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 1/2010

Dispersion of multi-walled carbon nanotubes in biocompatible dispersants

Occupational and consumer risk estimates for nanoparticles emitted by laser printers

Toxicological consequences of TiO2, SiC nanoparticles and multi-walled carbon nanotubes exposure in several mammalian cell types: an in vitro study

Process variables in biomimetic synthesis of silver nanoparticles by aqueous extract of Azadirachta indica (Neem) leaves

Study on magnetic fluid optical fiber devices for optical logic operations by characteristics of superparamagnetic nanoparticles and magnetic fluids

Molten salt synthesis of LaF3:Eu3+ nanoplates with tunable size and their luminescence properties

Premium Partners