In Vitro Multiple Solution Extracts from Leaves of Artemisia judaica L. Var. Sahariensis (L. Chevall.) Collected from the Algerian Sahara and Its Antimicrobial Activities Against Pathogenic Microorganisms

Water, Ethanolic, Methanolic, Ethyl acetate, Chloroform, and Petroleum ether extracts from leaves of Artimesia sahariensis found in the Algerian Sahara, traditionally used for the treatment of fever, diarrhea, gut infection, and other infectious diseases, were tested in vitro for their antimicrobial activity against pathogenic bacteria and fungi. Antibacterial and antifungal activities were tested using agar disk diffusion and agar dilution method. All the extracted products showed antimicrobial activity against the tested strains. Petroleum ether and Ethyl acetate extracts of A. sahariensis leaves showed high inhibitory activity against the majority of strains tested, with minimal inhibitory concentration (MIC) ranging from 30–32 µg/ml. The largest inhibition zone was obtained with chloroform extract against Pseudomonas syringae pv. Tomato ATCC 1086 (18,33 ± 0,58 mm) and the MIC value of 40 µg/ml was obtained. The results showed that Methanolic and Ethyl acetate extracts have potent antifungal activity against Fusarium spp. CLMAS 11 with a MIC value of 80 µg/ml and an inhibition zone ranging from 17, 0 ± 1, 0 to 17, 33 ± 0, 58, respectively. This study reveals clearly that A. sahariensis can serve as a potential source of antimicrobial compounds.