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2014 | OriginalPaper | Chapter

113. Isolation and Characterization of n-Butanol Tolerant Microorganisms

Authors : Yue Yu, Hongjiang Yang, Qun Li, Xuying Qin

Published in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Publisher: Springer Berlin Heidelberg

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Abstract

n-butanol is produced traditionally by acetone butanol ethanol (ABE) fermentation with relatively low productivity. To improve n-butanol synthesis level, microbes naturally with high tolerance to n-butanol are required for metabolic engineering. In this study, a total of 30 n-butanol tolerant microorganisms were isolated from various environmental samples by screening the enriched cultures. Ten of them were able to tolerate more than 2 % (v/v) n-butanol, and strain YY-23 even thrived in the presence of 3 % (v/v) n-butanol. In addition, n-butanol tolerance mechanisms were investigated by quantitatively analyzing degradation ability of the isolates. The results showed that strains YY-13, YY-14, YY-15, and YY-18 didn’t metabolize n-butanol in LB medium, suggesting that they didn’t have n-butanol degrading enzymes and all of them might be used as candidates for construction of high n-butanol producing strains. Strains YY-10 and YY-29 showed strong n-butanol degradation efficiency (31 and 20 %, respectively), and might be used for n-butanol bioremediation. Furthermore, the isolated strains were identified by the 16S rRNA gene sequences comparative analysis.

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Metadata
Title
Isolation and Characterization of n-Butanol Tolerant Microorganisms
Authors
Yue Yu
Hongjiang Yang
Qun Li
Xuying Qin
Copyright Year
2014
Publisher
Springer Berlin Heidelberg
Book
Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Print ISBN: 978-3-642-37921-5

Electronic ISBN: 978-3-642-37922-2

Copyright Year: 2014

DOI
https://doi.org/10.1007/978-3-642-37922-2_113

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