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Biology of the Physarum polycephalum Plasmodium: Preliminaries for Unconventional Computing Read chapter Read first chapter

2016 | OriginalPaper | Chapter

Long-Term Storable Microfluidic Whole-Cell Biosensor Using Physarum polycephalum for Toxicity Prescreening

Authors : Soicdhiro Tsuda, Klaus-Peter Zauner, Hywel Morgan

Published in: Advances in Physarum Machines

Publisher: Springer International Publishing

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Abstract

A storable whole-cell biosensor has been a challenge in the whole-cell biosensor research. We developed a long-term storable whole-cell biosensor using the true slime mould, Physarum polycephalum, for toxicity detection. The cell is interfaced to a microfluidic device with impedance measurement system. The oscillatory activity of the cell when exposed to various concentrations of 2,4-dinitrophenol (DNP) is investigated. It has been demonstrated that the Physarum cell can be dry-stored in the device for months and used as bionsensor after revived with rehydration. This is the first implementation of storable whole-cell biosensor for toxicity detection use, and it suggests that the development of long-term storable, and potentially portable, whole-cell biosensor for general toxicity prescreening is possible.

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previous chapter Slime Mould Nanotechnology
next chapter Routing Physarum “Signals” with Chemicals
Literature
1.
Adams, D.S.: Mechanisms of cell shape change: the cytomechanics of cellular response to chemical environment and mechanical loading. J. Cell Biol. 117(1), 83–93 (1992)CrossRef
2.
Allen, P.J., Price, W.H.: The relation between respiration and protoplasmic flow in the slime mold, Physarum polycephalum. Am. J. Bot. 37(5), 393–402 (1950)CrossRef
3.
Bjerketorp, J., Hakansson, S., Belkin, S., Jansson, J.K.: Advances in preservation methods: keeping biosensor microorganisms alive and active. Curr. Opin. Biotechnol. 17, 43–49 (2006)CrossRef
4.
Bousse, L.: Whole cell biosensors. Sens. Actuators B Chem. 34(1–3), 270–275 (1996)CrossRef
5.
Carter, R.M., Blake II, R.C., Nguyen, T.D., Bostanian, L.A.: Near real-time biosensor-based detection of 2,4-dinitrophenol. Biosens. Bioelectron. 18, 69–72 (2003)CrossRef
6.
Chin, B., Lesowitz, G.S., Bernstein, I.A.: A cellular model for studying accommodation to environmental stressors: protection and potentiation by cadmium and other metals. Environ. Res. 16, 432–442 (1978)CrossRef
7.
Harris, M.O., Cocoran, J.J.: Toxicological profile for dinitrophenols. Technical report, Agency for Toxic Substances and Disease, Public Health Service, U.S. Department of Health and Human Services (1995)
8.
Jump, J.A.: Studies on sclerotization in Physarum polycephalum. Am. J. Bot. 41, 561–567 (1954)CrossRef
9.
Kamiya, N.: Physical and chemical basis of cytoplasmic streaming. Annu. Rev. Plant Physiol. 32, 205–236 (1981)CrossRef
10.
Kamiya, N., Nakajima, H., Abe, S.: Physiology of the motive force of protoplasmic streaming. Protoplasma 48(1), 94–112 (1957)CrossRef
11.
Macey, P.: Impedance spectroscopy based interfacing with a living cell for biosensors and bio-coporcessors. Part III Project Report, School of Electronics and Computer Science, University of Southampton (2007)
12.
Mohberg, J.: The use of physarum for testing of toxicity/mutagenicity. Technical Report AFAMRL-TR-84-007, Air Force Aerospace Medical Research Laboratory (1984)
13.
Mulchandani, P., Hangarter, C.M., Lei, Y., Chen, W., Mulchandani, A.: Amperometric microbial biosensor for p-nitrophenol using Moraxella sp.-modified carbon paste electrode. Biosens. Bioelectron. 21, 523–527 (2005)
14.
Nakagaki, T., Yamada, H., Ueda, T.: Modulation of cellular rhythm and photoavoidance by oscillatory irradiation in the Physarum plasmodium. Biophys. Chem. 82, 23–28 (1999)
15.
Parker, J.A., Kenyon, R.V., Troxel, D.E.: Comparison of interpolating methods for image resampling. IEEE Trans. Med. Imaging 2(1), 31–39 (1983)CrossRef
16.
Sauer, H.W.: Developmental Biology of Physarum. Cambridge University Press (1981)
17.
Sørensen, S.J., Burmølle, M., Hansen, L.H.: Making bio-sense of toxicity: new developments in whole-cell biosensors. Curr. Opin. Biotechnol. 17, 11–16 (2006)CrossRef
18.
Sørensen, S.J., Hansen, L.H.: The use of whole-cell biosensors to detect and quantify compounds or conditions affecting biological systems. Microb. Ecol. 42, 483–494 (2001)CrossRef
19.
Sun, T., Morgan, H.: Impedance measurements of cells. In: Li, D. (ed.) Encyclopedia of Micro- and Nano-fluidics. Springer, Germany (2008)
20.
Takamatsu, A., Fujii, T.: Construction of a living coupled oscillator system of plasmodial slime mold by a microfabricated structure. Sens. Update 10(1), 33–46 (2002)CrossRef
21.
Takamatsu, A., Fujii, T., Endo, I.: Control of interaction strength in a network of the true slime mold by a microfabricated structure. BioSystems 55, 33–38 (2000)CrossRef
22.
Terayama, K., Honma, H., Kawarabayashi, T.: Toxicity of heavy metals and insecticides on slime mold Physarum plycephalum. J. Toxicol. Sci. 1978, 293–304 (1978)CrossRef
23.
Ueda, T., Terayama, K., Kurihara, K., Kobatake, Y.: Threshold phenomena in ghemoreception and taxis in slime mold Physarum polycephalum. J. Gen. Physiol. 65, 223–234 (1975)CrossRef
24.
Wohlfarth-Bottermann, K.E.: Oscillatory contraction activity in physarum. J. Exp. Biol. 81, 15–32 (1979)
25.
Yamada, Y., Inoue, A., Watanabe, S.: 2, 4-Dinitrophenol as a specific inhibitor of the breakdown of the actomyosin-phosphate-ADP complex. J. Biochem. 80(5), 1109–1115 (1976)
Metadata
Title
Long-Term Storable Microfluidic Whole-Cell Biosensor Using Physarum polycephalum for Toxicity Prescreening
Authors
Soicdhiro Tsuda
Klaus-Peter Zauner
Hywel Morgan
Copyright Year
2016
Book
Advances in Physarum Machines

Print ISBN: 978-3-319-26661-9

Electronic ISBN: 978-3-319-26662-6

Copyright Year: 2016

DOI
https://doi.org/10.1007/978-3-319-26662-6_8

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