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Measurement Techniques
Published in: Measurement Techniques 3/2012

01-06-2012 | Medical and Biological Measurements

Metrological support for DNA sequencing

Authors: S. S. Golubev, S. A. Kononogov, Yu. A. Kudeyarov, A. V. Mardanov, P. Yu. Nikolaeva, N. V. Ravin, K. G. Skryabin

Published in: Measurement Techniques | Issue 3/2012

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Abstract

We discuss the operating principle of a pyrosequencer, which is an instrument for sequential reading of the nucleotide sequence of DNA molecules in pyrophosphate sequencing (pyrosequencing). We formulate the basic principles of metrological support for DNA sequencing and we present the results of studies of the metrological characteristics of the GS FLX pyrosequencer. We list the major problems encountered in designing a system for metrological support of DNA sequencing.
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Literature
1.
M. Margulies et al., “Genome sequencing in microfabricated high-density picolitre reactors,” Nature, No. 437, 376–380 (2005).
2.
S. S. Golubev et al., “Selection of a reference standard for determination of DNA nucleotide sequences,” Izmer. Tekhn., No. 12, 45–47 (2011).
3.
Federal Law of the Russian Federation No. 102-FZ, Assurance of the Uniformity in Measurement, Chap. 1, Sec. 2.
4.
International Vocabulary of Metrology. Basic and General Concepts and Associated Terms [Russian translation], Professional NPO, St. Petersburg (2010).
5.
V. A. Kuznetsov, L. K. Isaev, and I. A. Shaiko, Metrology [in Russian], STANDARTINFORM, Moscow (2005).
Metadata
Title
Metrological support for DNA sequencing
Authors
S. S. Golubev
S. A. Kononogov
Yu. A. Kudeyarov
A. V. Mardanov
P. Yu. Nikolaeva
N. V. Ravin
K. G. Skryabin
Publication date
01-06-2012
Publisher
Springer US
Published in
Measurement Techniques / Issue 3/2012
Print ISSN: 0543-1972
Electronic ISSN: 1573-8906
DOI
https://doi.org/10.1007/s11018-012-9962-6

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