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Journal of Nanoparticle Research

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01-07-2013 | Research Paper

Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells

Authors: L. A. Strobel, N. Hild, D. Mohn, W. J. Stark, A. Hoppe, U. Gbureck, R. E. Horch, U. Kneser, A. R. Boccaccini

Published in: Journal of Nanoparticle Research | Issue 7/2013

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Abstract

The present study investigates a new family of bioactive glass nanoparticles with and without Sr-doping focusing on the influence of the nanoparticles on human bone marrow stromal cells (hBMSCs) in vitro. The bioactive glass nanoparticles were fabricated by flame spray synthesis and a particle diameter of 30–35 nm was achieved. Glass nanoparticles were undoped (BG 13-93-0Sr) or doped with 5 wt% strontium (Sr) (BG 13-93-5Sr) and used at concentrations of 10 and 100 μg/cm² (particles per culture plate area), respectively. Cells were cultured for 14 days after which the samples were analysed regarding metabolic activity and expression of various bone-specific genes. Cell growth and morphology indicated the high cytocompatibility of the nanoparticulate bioactive glass. The presence of the nanoparticles enhanced cell growth compared to the plain polystyrene control group. At a concentration of 100 μg/cm², Sr-doped particles led to significantly enhanced gene expression of osteocalcin, collagen type 1 and vascular endothelial growth factor. Thus, Sr-doped nanoparticles showing a dose-dependent increase of osteogenic differentiation in hBMSCs are a promising biomaterial for bone regeneration purposes.

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Title: Novel strontium-doped bioactive glass nanoparticles enhance proliferation and osteogenic differentiation of human bone marrow stromal cells
Authors: L. A. Strobel
N. Hild
D. Mohn
W. J. Stark
A. Hoppe
U. Gbureck
R. E. Horch
U. Kneser
A. R. Boccaccini
Publication date: 01-07-2013
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 7/2013
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-013-1780-5

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