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Published in:
2017 | OriginalPaper | Chapter
12. Optimization and Design of Construction Biotechnology Processes
Authors : Volodymyr Ivanov, Viktor Stabnikov
Published in: Construction Biotechnology
Publisher: Springer Singapore
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Abstract
New types of construction material, biocement, or biogrouts, are developing extensively as an alternative to cement and chemical grouts. Biocement/biogrout forms calcium carbonate minerals due to activity of urease-producing bacteria (UPB) in the presence of urea and calcium ions. So, UPB play a role of a bioagent in the biocementation. The important task is the development of biotechnology to produce UPB biomass for large-scale biocementation/bioclogging applications. Hydrolyzed activated sludge can be used as a cheap medium for cultivation of urease-producing bacteria. Bacteria Yaniella sp. VS8 produced inducible urease and Bacillus sp. VS1 produced constitutive urease. Biomass of both strains can be grown in the cheap medium from hydrolyzed activated sludge without any supplementary components for large-scale applications of biocementation.