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2018 | OriginalPaper | Chapter

Optoelectronic System-on-Glass for On-Chip Detection of Fluorescence

Authors : N. Lovecchio, F. Costantini, M. Nardecchia, G. Petrucci, M. Tucci, P. Mangiapane, A. Nascetti, G. de Cesare, D. Caputo

Published in: Sensors and Microsystems

Publisher: Springer International Publishing

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Abstract

In this paper, we present an optoelectronic system-on-glass (SoG), suitable for detection of fluorescent molecule. It integrates, on the same glass substrate, an array of amorphous silicon (a-Si:H) photosensors and a thin film interferential filter. The system can be directly coupled with another glass substrate hosting a polydimethylsiloxane based microfluidic network where the fluorescent phenomena occur. The compatibility of the different technological steps to attain on the same glass substrate the photosensors and the filter determined the sequence, the selection of materials and the deposition parameters of the whole process. The electro-optical characterization of the photodiode, performed after the filter deposition, demonstrated the efficacy of the filter in reducing the excitation light. The system has been successfully tested using the ruthenium complex [Ru(phen)2(dppz)]2+, a fluorescent dye which works as DNA intercalating molecule.

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previous chapter Design of an Evanescent Waveguide Sensor Based on a-Si:H Photodiodes for Lab-on-Chip Applications
next chapter Optical Detection of Antioxidant Capacity in Food Using Metal Nanoparticles Formation. Study on Saffron Constituents
Literature
1.
Parks, J.W., Olson, M.A., Kim, J., Ozcelik, D., Cai, H., Carrion Jr., R., Patterson, J.L., Mathies, R.A., Hawkins, A.R., Schmidt, H.: Integration of programmable microfluidics and on-chip fluorescence detection for biosensing applications. Biomicrofluidics 8(5), 054111 (2014)CrossRef
2.
Lim, J., Gruner, P., Konrad, M., Baret, J.C.: Micro-optical lens array for fluorescence detection in droplet-based microfluidics. Lab Chip 13(8), 1472–1475 (2013)CrossRef
3.
Ryu, G., Huang, J., Hofmann, O., Walshe, C.A., Sze, J.Y., McClean, G.D., Mosley, A., Rattle, S.J., deMello, J.C., deMello, A.J., Bradley, D.D.: Highly sensitive fluorescence detection system for microfluidic lab-on-a-chip. Lab Chip 11(9), 1664–1670 (2011)CrossRef
4.
Xi, Q., Zhou, D.M., Kan, Y.Y., Ge, J., Wu, Z.K., Yu, R.Q., Jiang, J.H.: Highly sensitive and selective strategy for microRNA detection based on WS2 nanosheet mediated fluorescence quenching and duplex-specific nuclease signal amplification. Anal. Chem. 86(3), 1361–1365 (2014)CrossRef
5.
Caputo, D., de Angelis, A., Lovecchio, N., Nascetti, A., Scipinotti, R., de Cesare, G.: Amorphous silicon photosensors integrated in microfluidic structures as a technological demonstrator of a “true” Lab-on-Chip system. Sens. Bio-Sens. Res. 3, 98–104 (2015)CrossRef
6.
Abgrall, P., Gue, A.M.: Lab-on-chip technologies: making a microfluidic network and coupling it into a complete microsystem—a review. J. Micromech. Microeng. 17(5), R15 (2007)CrossRef
7.
Petrucci, G., Caputo, D., Lovecchio, N., Costantini, F., Legnini, I., Bozzoni, I., Nascetti, A., de Cesare, G.: Multifunctional system-on-glass for Lab-on-chip applications. Biosens. Bioelectron. 93, 315–321 (2016)
8.
Zhu, H., Isikman, S.O., Mudanyali, O., Greenbaum, A., Ozcan, A.: Optical imaging techniques for point-of-care diagnostics. Lab Chip 13(1), 51–67 (2013)CrossRef
9.
Caputo, D., Ceccarelli, M., de Cesare, G., Nascetti, A., Scipinotti, R.: Lab-on-glass system for DNA analysis using thin and thick film technologies. In MRS Proceedings, vol. 1191, pp. 1191-OO06. Cambridge University Press (2009)
10.
Song, Y., Gyarmati, P., Araújo, A.C., Lundeberg, J., Brumer III, H., Ståhl, P.L.: Visual detection of DNA on paper chips. Anal. Chem. 86(3), 1575–1582 (2014)CrossRef
11.
Ahrberg, C.D., Ilic, B.R., Manz, A., Neužil, P.: Handheld real-time PCR device. Lab Chip 16(3), 586–592 (2016)CrossRef
12.
McKinley, A.W., Lincoln, P., Tuite, E.M.: Sensitivity of [Ru (phen) 2 dppz] 2+light switch emission to ionic strength, temperature, and DNA sequence and conformation. Dalton Trans. 42(11), 4081–4090 (2013)CrossRef
13.
Caputo, D., Costantini, F., Lovecchio, N., Viri, V., Tucci, M., Mangiapane, P., Ruggi, A., Petrucci, G., Nascetti, A., de Cesare, G.: Highly miniaturized system for on-chip detection of DNA. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society (2016)
14.
Martins, S.A.M., Moulas, G., Trabuco, J.R.C., Monteiro, G.A., Chu, V., Conde, J.P., Prazeres, D.M.F.: Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters. Biosens. Bioelectron. 52, 232–238 (2014)CrossRef
15.
Mirasoli, M., Nascetti, A., Caputo, D., Zangheri, M., Scipinotti, R., Cevenini, L., de Cesare, G., Roda, A.: Multiwell cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection: development, characterization and comparison with cooled-CCD luminograph. Anal. Bioanal. Chem. 406(23), 5645–5656 (2014)CrossRef
16.
Costantini, F., Sberna, C., Petrucci, G., Reverberi, M., Domenici, F., Fanelli, C., Manetti, C., de Cesare, G., Derosa, M., Nascetti, A., Caputo, D.: Aptamer-based sandwich assay for on chip detection of Ochratoxin A by an array of amorphous silicon photosensors. Sens. Actuators B: Chem. 230, 31–39 (2016)CrossRef
17.
Costantini, F., Nascetti, A., Scipinotti, R., Domenici, F., Sennato, S., Gazza, L., Bordi, F., Pogna, N., Manetti, C., Caputo, D., de Cesare, G.: On-chip detection of multiple serum antibodies against epitopes of celiac disease by an array of amorphous silicon sensors. RSC Adv. 4(4), 2073–2080 (2014)CrossRef
18.
Costantini, F., Sberna, C., Petrucci, G., Manetti, C., de Cesare, G., Nascetti, A., Caputo, D.: Lab-on-chip system combining a microfluidic-ELISA with an array of amorphous silicon photosensors for the detection of celiac disease epitopes. Sens. Bio-Sens. Res. 6, 51–58 (2015)CrossRef
19.
Caputo, D., de Cesare, G., Scipinotti, R., Stasio, N., Costantini, F., Manetti, C., Nascetti, A.: On-chip diagnosis of celiac disease by an amorphous silicon chemiluminescence detector. In Sensors and Microsystems, pp. 183–187. Springer International Publishing, Berlin (2014)
Metadata
Title
Optoelectronic System-on-Glass for On-Chip Detection of Fluorescence
Authors
N. Lovecchio
F. Costantini
M. Nardecchia
G. Petrucci
M. Tucci
P. Mangiapane
A. Nascetti
G. de Cesare
D. Caputo
Copyright Year
2018
Book
Sensors and Microsystems

Print ISBN: 978-3-319-66801-7

Electronic ISBN: 978-3-319-66802-4

Copyright Year: 2018

DOI
https://doi.org/10.1007/978-3-319-66802-4_20