2011 | OriginalPaper | Chapter
Quantitative Phase Imaging in Microscopy Using a Spatial Light Modulator
Authors : Vicente Micó, Javier García, Luis Camacho, Zeev Zalevsky
Published in: Coherent Light Microscopy
Publisher: Springer Berlin Heidelberg
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In this chapter, we present a new method capable of recovery of the
quantitative phase
Quantitative phase
information of microscopic samples. Essentially, a
spatial light modulator (SLM)
Spatial light modulator (SLM)
and digital image processing are the basics to extract the sample’s phase distribution. The
SLM
SLM
produces a set of misfocused images of the input sample at the CCD plane by displaying a set of lenses with different power at the
SLM
SLM
device. The recorded images are then numerically processed to retrieve phase information. Computations are based on the wave propagation equation and lead to a complex amplitude image containing information of both amplitude and phase distributions of the input sample diffracted wave front. The proposed configuration becomes a non-interferometric architecture (conventional transmission
imaging
Imaging
mode) where no moving elements are included. Experimental results are provided in comparison with conventional digital holographic microscopy.