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2014 | OriginalPaper | Chapter

120. Screening of Antimicrobial Marine Microorganisms and Purifying of Its Bioactive Substances

Authors : Zhiwen Liu, Qiankun Ruan, Sirigulen Qian, Lina Cong

Published in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Publisher: Springer Berlin Heidelberg

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Abstract

A bacterium HS-A38 with antimicrobial activities was isolated from the intestine of wild sea cucumbers in Dalian sea area. Based on the analysis of morphological, physiological, and 16S rDNA sequence, the strain HS-A38 was identified as Bacillus subtilis. Two bioactive substances (1 and 2) were purified from the fermented broth of the Bacillus subtilis HS-A38 using the methods of fractional sedimentation with ammonium sulfate, CM-52 ion-exchange chromatography and Sephadex G-75 column. The SDS-PAGE analysis indicated that the relative molecular weight of these bioactive substances were 41 kDa and 28 kDa, respectively. The antibacterial spectra showed that substance 1 could only inhibit Gram-positive bacteria, whereas substance 2 could significantly inhibit both the Gram-positive and negative bacteria.

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Literature
1.
Hibbing ME, Fuqua C, Parsek MR et al (2010) Bacterial competition: surviving and thriving in the microbial jungle. Nat Rev Microbiol 8:15–25CrossRef
2.
Demain AL, Sanchez S (2009) Microbial drug discovery: 80 years of progress. J Antibiot 62:5–16CrossRef
3.
Fischbach MA (2009) Antibiotics from microbes: converging to kill. Curr Opin Microbiol 12:520–527CrossRef
4.
5.
Nair S, Simidu U (1987) Distribution and significance of heterotrophic marine bacteria with antibacterial activity. Appl Environ Microbiol 53:2957–2962
6.
Long E, Azam F (2001) Antagonistic interactions among marine pelagic bacteria. Appl Environ Microbiol 67:4975–4983CrossRef
7.
Zhang LX, An R, Wang JP et al (2005) Exploring novel bioactive compounds from marine microbes. Curr Opin Microbiol 8:276–281CrossRef
8.
Gram L, Melchiorsen J, Bruhn JB (2010) Antibacterial activity of marine culturable bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms. Mar Biotechnol 12:439–451CrossRef
9.
Hohmann C, Schneider K, Bruntner C et al (2009) Caboxamycin, a new antibiotic of the benzoxazole family produced by the deep-sea strain streptomyces sp NTK 937. J Antibiot 62:99–104CrossRef
10.
Taylor MW, Radax R, Steger D et al (2007) Sponge-associated microorganisms: evolution, ecology, and biotechnological potential. Microbiol Mol Biol Rev 71:295–347CrossRef
11.
Wiese J, Thiel V, Nagel K et al (2009) Diversity of antibiotic-active bacteria associated with the brown alga laminaria saccharina from the baltic sea. Mar Biotechnol 11:287–300CrossRef
12.
Rypien KL, Ward JR, Azam F (2010) Antagonistic interactions among coral-associated bacteria. Environ Microbiol 12:28–39CrossRef
13.
Herndl H, Wiese J, Thiel V et al (2010) Phylogenetic diversity and antimicrobial activities of bryozoan-associated bacteria isolated from mediterranean and baltic sea habitats. Syst Appl Microbiol 33:94–104CrossRef
14.
Bowman JP (2007) Bioactive compound synthetic capacity and ecological significance of marine bacterial genus pseudoalteromonas. Mar Drugs 5:220–241CrossRef
15.
Martens T, Gram L, Grossart HP et al (2007) Bacteria of the roseobacter clade show potential for secondary metabolite production. Microb Ecol 54:31–42CrossRef
16.
Bull JE (2007) Stach marine actinobacteria: new opportunities for natural product search and discovery. Trends Microbiol 15:491–499CrossRef
17.
Andersen RJ, Wolfe MS, Faulkner DJ (1974) Autotoxic antibiotic production by a marine chromobacterium. Mar Biol 27:281–285CrossRef
18.
Geng HF, Bruhn JB, Nielsen KF et al (2008) Genetic dissection of tropodithietic acid biosynthesis by marine roseobacters. Appl Environ Microbiol 74:1535–1545CrossRef
19.
Romanenko LA, Uchino M, Kalinovskaya NI et al (2008) Isolation, phylogenetic analysis and screening of marine mollusc-associated bacteria for antimicrobial, hemolytic and surface activities. Microbiol Res 163:633–644CrossRef
20.
Das P, Mukherjee S, Sen R (2008) Antimicrobial potential of a lipopeptide biosurfactant derived from a marine bacillus circulans. J Appl Microbiol 104:1675–1684CrossRef
21.
Kiran GS, Thomas TA, Selvin J (2010) Production of a new glycolipid biosurfactant from marine nocardiopsis lucentensis MSA04 in solid-state cultivation. Colloids Surf B Biointerfaces 78:8–16CrossRef
22.
Gomez D, Espinosa E, Bertazzo M et al (2008) The macromolecule with antimicrobial activity synthesized by pseudoalteromonas luteoviolacea strains is an L-amino acid oxidase. Appl Microbiol Biotechnol 79:925–930CrossRef
23.
Penesyan A, Marshall-Jones Z, Holmstrom C et al (2009) Antimicrobial activity observed among cultured marine epiphytic bacteria reflects their potential as a source of new drugs: research article. FEMS Microbiol Ecol 69:113–124CrossRef
24.
Hentschel U, Schmid M, Wagner M et al (2001) Isolation and phylogenetic analysis of bacteria with antimicrobial activities from the mediterranean sponges aplysina aerophoba and aplysina cavernicola. FEMS Microbiol Ecol 35:305–312CrossRef
25.
Muscholl-Silberhorn A, Thiel V, Imhoff J (2009) Abundance and bioactivity of cultured spongesssociated bacteria from the mediterranean sea. Microb Ecol 55:94–106CrossRef
26.
Egan S, Thomas T, Kjelleberg S (2008) Unlocking the diversity and biotechnological potential of marine surface associated microbial communities. Curr Opin Microbiol 11:219–225CrossRef
27.
Cong LN, Yang XJ, Wang XX et al (2009) Characterization of an i-type lysozyme gene from the sea cucumber stichopus japonicus, and enzymatic and nonenzymatic antimicrobial activities of its recombinant protein. J Bios Bioeng 107:583–588CrossRef
Metadata
Title
Screening of Antimicrobial Marine Microorganisms and Purifying of Its Bioactive Substances
Authors
Zhiwen Liu
Qiankun Ruan
Sirigulen Qian
Lina Cong
Copyright Year
2014
Publisher
Springer Berlin Heidelberg
Book
Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Print ISBN: 978-3-642-37921-5

Electronic ISBN: 978-3-642-37922-2

Copyright Year: 2014

DOI
https://doi.org/10.1007/978-3-642-37922-2_120

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