28. Spectroscopic Characterization of a New Antibacterial Material for Sensing Applications

A novel antibacterial system based on immobilization of glucose oxidase enzyme (GOx) in a poly(vinyl alcol) (PVA) film was proposed. The GOx/PVA composite material has been extensively characterized by UV-vis and X-ray photoelectron (XPS) spectroscopy to verify the preservation of the enzyme structural integrity and of the enzymatic activity in PVA membrane. Moreover, XPS characterization was used to analyze the chemistry of GOx/PVA composite film. The antibacterial lysozyme-like activity of GOx/PVA was evaluated by a standard assay on Petri dishes employing Micrococcus luteus cell walls. Thus the findings from this study have implications for future investigations related to employment of GOx/PVA as a compound of pharmaceutical and technological interest.