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Journal of Nanoparticle Research

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01-06-2010 | Research Paper

Synthesis of bombesin-functionalized iron oxide nanoparticles and their specific uptake in prostate cancer cells

Authors: Amanda L. Martin, Jennifer L. Hickey, Amber L. Ablack, John D. Lewis, Leonard G. Luyt, Elizabeth R. Gillies

Published in: Journal of Nanoparticle Research | Issue 5/2010

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Abstract

The imaging of molecular markers associated with disease offers the possibility for earlier detection and improved treatment monitoring. Receptors for gastrin-releasing peptide are overexpressed on prostate cancer cells offering a promising imaging target, and analogs of bombesin, an amphibian tetradecapeptide have been previously demonstrated to target these receptors. Therefore, the pan-bombesin analog [β-Ala11, Phe13, Nle14]bombesin-(7–14) was conjugated through a linker to dye-functionalized superparamagnetic iron oxide nanoparticles for the development of a new potential magnetic resonance imaging probe. The peptide was conjugated via click chemistry, demonstrating a complementary alternative methodology to conventional peptide-nanoparticle conjugation strategies. The peptide-functionalized nanoparticles were then demonstrated to be selectively taken up by PC-3 prostate cancer cells relative to unfunctionalized nanoparticles and this uptake was inhibited by the presence of free peptide, confirming the specificity of the interaction. This study suggests that these nanoparticles have the potential to serve as magnetic resonance imaging probes for the detection of prostate cancer.

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Title: Synthesis of bombesin-functionalized iron oxide nanoparticles and their specific uptake in prostate cancer cells
Authors: Amanda L. Martin
Jennifer L. Hickey
Amber L. Ablack
John D. Lewis
Leonard G. Luyt
Elizabeth R. Gillies
Publication date: 01-06-2010
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 5/2010
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-009-9681-3

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