Top

Published in:

2022 | OriginalPaper | Chapter

The Influence of High D-glucose Concentrations on Increasing the Expression of EGR-1, PTEN and GGPS-1 Involved in Insulin Resistance of AT-MSCs

Authors : Huu-Phuong Mai, Nhu-Thuy Trinh, Vong Binh Long, Nguyen Trong Binh, Dang-Quan Nguyen, Hoa-Xo Duong

Published in: 8th International Conference on the Development of Biomedical Engineering in Vietnam

Publisher: Springer International Publishing

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Type 2 diabetes mellitus (T2DM) is increasing worldwide, characterized by insulin resistance and hyperglycemia. The results of previous studies have demonstrated that high D-glucose concentrations alter the characteristic and function of adipose tissue-derived mesenchymal stem cells (AT-MSCs). Besides, early growth response factor-1 (EGR-1) and insulin resistance mediators (PTEN and GGPS-1) were highly upregulated in diabetic AT-MSCs (dAT-MSCs) compared with non-diabetic AT-MSCs (nAT-MSCs). In this research, we examined the effect of high glucose concentrations on nAT-MSCs in comparison to dAT-MSCs on the expression of EGR-1, PTEN, and GGPS-1 involved in insulin resistance of human AT-MSCs. The expression of insulin resistance-related genes and EGR-1 protein were analyzed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blot, respectively. The results show that the high D-Glucose concentrations (25, 50, and 100 mM) enhanced the expression of EGR-1 and insulin resistance-related genes in nAT-MSCs compared with non-treated nAT-MSCs and dAT-MSCs. Notably, the increase of high D-Glucose concentration remarkably upregulated the expression of EGR-1, PTEN, and GGPS-1 in both nAT-MSCs and dAT-MSCs. The effect of high D-glucose concentration (100 mM) remarkably increased the expression of EGR-1, PTEN, and GGPS-1 in human AT-MSCs. The results of this study will expand our knowledge about the impact of high glucose concentration on insulin resistance in human AT-MSCs for the improvement in diabetic treatment.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 340 Zeitschriften

aus folgenden Fachgebieten:

Bauwesen + Immobilien
Business IT + Informatik
Finance + Banking
Management + Führung
Marketing + Vertrieb
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

previous chapter Purification of Alpha-Fetoprotein from Human Umbilical Cord Plasma

next chapter Antioxidant Effect of Syringic Acid Grafted Chitooligosaccharides in RAW264.7 Cells

Cramer EFC, Jones RK, Slakey DP, Dupin CL, Newsome ER, Alt EU, Izadpanah R (2010) Persistent high glucose concentrations alter the regenerative potential of mesenchymal stem cells. Stem Cells Dev 19:1875–1884. https://doi.org/10.1089/scd.2010.0009CrossRef

Groop L, DeFronzo RA, Ferrannini E, Henry RR, Herman WH, Holst JJ, Hu FB, Kahn CR, Raz I, Shulman GI, Simonson DC (2015). Type 2 diabetes mellitus. Nat Rev. https://doi.org/10.1038/nrdp.2015.19

Xiao S, Pillay TS, Olefsky JM (1996) Glucose-induced phosphorylation of the insulin receptor—functional effects and characterization of phosphorylation sites. Am Soc Clinx Invest 97:613–620. https://doi.org/10.1172/JCI118457CrossRef

Renström JBF, Svensson M, Eriksson JW (2007) Insulin resistance induced by high glucose and high insulin precedes insulin receptor substrate 1 protein depletion in human adipocytes. Metab Clin Exp 56:190–198. https://doi.org/10.1016/j.metabol.2006.09.012CrossRef

Ning XY, Shen F-Y, Gao X, Xue B, Li C-J (2011) An early response transcription factor, Egr-1, enhances insulin resistance in type 2 diabetes with chronic hyperinsulinism. J Biol Chem 286:14508–14515. https://doi.org/10.1074/jbc.M110.190165CrossRef

Ning Shen Xiao Yu, Zhang M-L, Pan F-Y, Wang C, Jia W-P, Liu C, Gao Q, Gao X, Xue B, Li C-J (2011) Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in mice. EMBO J 30:3754–3765. https://doi.org/10.1038/emboj.2011.277CrossRef

Trinh T-T, Ohneda K, Kimura K, Salazar GT, Sato F, Ohneda O (2016) Increased expression of EGR-1 in diabetic human adipose tissue-derived mesenchymal stem cells reduces their wound healing capacity. Stem Cells Dev 25:760–773. https://doi.org/10.1089/scd.2015.0335CrossRef

Kern HES, Stoeve J, Klüter H, Bieback K (2006) Comparative analysis of mesenchymal stem cells from bone marrow, umbilical cord blood, or adipose tissue. Stem Cells 24:1294–1301. https://doi.org/10.1634/stemcells.2005-0342CrossRef

Benisch P, Schilling T, Li YM, Zeck S, Meissner-Weigl J, Schneider D, Limbert C, Seufert J, Kassem M, Schütze N, Jakob F (2007) Effects of high glucose on mesenchymal stem cell proliferation and differentiation. Biochem Biophys Res Commun 363:209–215. https://doi.org/10.1016/j.bbrc.2007.08.161

10.

Kimura MNK, Salazar G, Yamashita T, Tsuboi I, Mishima H, Matsushita S, Sato F, Yamagata K, Ohneda O (2014) The role of CCL5 in the ability of adipose tissue-derived mesenchymal stem cells to support repair of ischemic regions. Stem Cells Dev 23:488–501. https://doi.org/10.1089/scd.2013.0307CrossRef

11.

Seeberger KL, Yeung TY, Kin T, Adesida A, Jomha N, James Shapiro AM, Korbutt GS (2012) Human mesenchymal stem cells protect human islets from pro-inflammatory cytokines. PLoS One 7. https://doi.org/10.1371/journal.pone.0038189

12.

Trinh TYNT, Tran Cam Tu, Kato T, Ohneda K, Sato F, Ohneda O (2016) Microvesicles enhance the mobility of human diabetic adipose tissuederived mesenchymal stem cells in vitro and improve wound healing in vivo. Biochem Biophys Res Commun 473:1111–1118. https://doi.org/10.1016/j.bbrc.2016.04.025CrossRef

13.

Aguiari BZSLP, Vindigni V, Rimessi A, Bianchi K, Franzin C, Cortivo R, Rossato M, Vettor R, Abatangelo G, Pozzan T, Pinton P, Rizzuto R (2008) High glucose induces adipogenic differentiation of muscle-derived stem cells. PNAS 105:1226–1231. https://doi.org/10.1073/pnas.0711402105CrossRef

14.

Jianming HH, Guo JG, Bai H, He H, Assi R, Isaji T, Wang T, Setia O, Lopes L, Yongquan Gu, Dardik A (2018) Adipose-derived mesenchymal stem cells accelerate diabetic wound healing in a similar fashion as bone marrow-derived cells. Am J Physiol Cell Physiol 315:885–896. https://doi.org/10.1152/ajpcell.00120.2018CrossRef

15.

Rosivatz E (2007) Inhibiting PTEN. Biochem Soc Trans 35:257–259. https://doi.org/10.1042/BST0350257CrossRef

16.

Dey CS, Gupta A (2012) PTEN, a widely known negative regulator of insulin/PI3K signaling, positively regulates neuronal insulin resistance. Mol Biol Cell 23:3882–3898. https://doi.org/10.1091/mbc.E12-05-0337

17.

SørensenLR, Josefsen K, Buschard K, Birkenbach M (1999) Glucose induces early growth response gene (Egr-1) expression in pancreatic beta cells. Diabetologia 42:195–203. https://doi.org/10.1007/s001250051139

18.

Castoldi M, Horn P, Wagner W, Diehlmann A, Bork S, Saffrich R, Benes V, Blake J, Pfister S, Eckstein V, Ho AD (2008) Replicative senescence of mesenchymal stem cells: a continuous and organized process. PLoS ONE 3. https://doi.org/10.1371/journal.pone.0002213

19.

Munsey TS, Abuarab N, Jiang L-H,Li J, Sivaprasadarao A (2017) High glucose-induced ROS activates TRPM2 to trigger lysosomal membrane permeabilization and Zn²⁺-mediated mitochondrial fission. Sci Signal 10. https://doi.org/10.1126/scisignal.aal4161

20.

Song G, Cao X, Shao X, Zhao Y, Shi B (2014) Metformin rescues the MG63 osteoblasts against the effect of high glucose on proliferation. J Diabetes Res 2014. https://doi.org/10.1155/2014/453940

21.

Rukhsana SP, Hasan N, Harada S (2003) Differential regulation of early growth response gene-1 expression by insulin and glucose in vascular endothelial cells, arteriosclerosis. Thromb Vasc Biol 23:988–993. https://doi.org/10.1161/01.ATV.0000071351.07784.19CrossRef

Title: The Influence of High D-glucose Concentrations on Increasing the Expression of EGR-1, PTEN and GGPS-1 Involved in Insulin Resistance of AT-MSCs
Authors: Huu-Phuong Mai
Nhu-Thuy Trinh
Vong Binh Long
Nguyen Trong Binh
Dang-Quan Nguyen
Hoa-Xo Duong
Publisher: Springer International Publishing
Book: 8th International Conference on the Development of Biomedical Engineering in Vietnam
Print ISBN: 978-3-030-75505-8

Electronic ISBN: 978-3-030-75506-5

Copyright Year: 2022
DOI: https://doi.org/10.1007/978-3-030-75506-5_42

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Springer Professional "Wirtschaft"

Premium Partner