Skip to main content
Top

2018 | OriginalPaper | Chapter

Unsupervised Parametrization of Nano-Objects in Electron Microscopy

Authors : Pavla Urbanová, Norbert Cyran, Pavel Souček, Antonín Bárta, Vladyslav Bozhynov, Dinara Bekkhozhayeva, Petr Císař, Miloš Železný

Published in: Bioinformatics and Biomedical Engineering

Publisher: Springer International Publishing

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

The observation of the nano sized objects in electron microscopy is demanding an automation of evaluation of captured images. The analysis of the digital images should be focused on objects detection, classification, and parametrization. In this work, three different examples of bioinformatical tasks are presented and discussed. The sphericity of such objects is one of the key parameter in nano object detections. The parametrization has to deal with specific properties of electron microscopy images, like high level of noise, low contrast, uneven background, and few pixels per objects. The presented approach combines unsupervised filtration and automatic object detection. The result is the software application with simple graphic user interface.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 340 Zeitschriften

aus folgenden Fachgebieten:

  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Versicherung + Risiko




Jetzt Wissensvorsprung sichern!

Literature
1.
go back to reference Von Byern, J., Dorrer, V., Merritt, D.J., Chandler, P., Stringer, I., Marchetti-Deschmann, M., McNaughton, A., Cyran, N., Thiel, K., Noeske, M., Grunwald, I.: Characterization of the fishing lines in titiwai (Arachnocampa luminosa Skuse, 1890) from New Zealand and Australia. PLoS ONE 11(12), e0162687 (2016)CrossRef Von Byern, J., Dorrer, V., Merritt, D.J., Chandler, P., Stringer, I., Marchetti-Deschmann, M., McNaughton, A., Cyran, N., Thiel, K., Noeske, M., Grunwald, I.: Characterization of the fishing lines in titiwai (Arachnocampa luminosa Skuse, 1890) from New Zealand and Australia. PLoS ONE 11(12), e0162687 (2016)CrossRef
2.
go back to reference Urban, J., Cisar, P., Pautsina, A., Soukup, J., Barta, A.: Discrete representation and morphology. In: Technical Computing Prague, p. 322 (2013) Urban, J., Cisar, P., Pautsina, A., Soukup, J., Barta, A.: Discrete representation and morphology. In: Technical Computing Prague, p. 322 (2013)
3.
go back to reference Vanek, J., Urban, J., Gardian, Z.: Automated detection of photosystems II in electron microscope photographs. In: Technical Computing Prague, p. 102 (2006) Vanek, J., Urban, J., Gardian, Z.: Automated detection of photosystems II in electron microscope photographs. In: Technical Computing Prague, p. 102 (2006)
4.
go back to reference Duda, R.O., Hart, P.E.: Use of the Hough transformation to detect lines and curves in pictures. Commun. ACM 15(1), 11–15 (1972)CrossRefMATH Duda, R.O., Hart, P.E.: Use of the Hough transformation to detect lines and curves in pictures. Commun. ACM 15(1), 11–15 (1972)CrossRefMATH
5.
go back to reference Urban, J.: Automatic Image Segmentation of HeLa Cells in Phase Contrast Microphotography. Lap LAMBERT Academic Publishing, Saarbrucken (2012) Urban, J.: Automatic Image Segmentation of HeLa Cells in Phase Contrast Microphotography. Lap LAMBERT Academic Publishing, Saarbrucken (2012)
6.
go back to reference Beucher, S.: Applications of mathematical morphology in material sciences: a review of recent developments. In: International Metallography Conference, pp. 41–46 (1995) Beucher, S.: Applications of mathematical morphology in material sciences: a review of recent developments. In: International Metallography Conference, pp. 41–46 (1995)
7.
go back to reference Otsu, N.: A threshold selection method from gray-level histogram. IEEE Trans. Syst. Man Cybern. SMC–9, 62–66 (1979)CrossRef Otsu, N.: A threshold selection method from gray-level histogram. IEEE Trans. Syst. Man Cybern. SMC–9, 62–66 (1979)CrossRef
8.
go back to reference Slot, J.W., Geuze, H.J.: A new method of preparing gold probes for multiple-labeling cytochemistry. Eur. J. Cell Biol. 38(1), 87–93 (1985) Slot, J.W., Geuze, H.J.: A new method of preparing gold probes for multiple-labeling cytochemistry. Eur. J. Cell Biol. 38(1), 87–93 (1985)
9.
go back to reference Sonka, M., Hlavac, V., Boyle, R.: Image Processing, Analysis, and Machine Vision. Cole Publishing Company, Brooks (1999) Sonka, M., Hlavac, V., Boyle, R.: Image Processing, Analysis, and Machine Vision. Cole Publishing Company, Brooks (1999)
10.
go back to reference Gonzalez, R.C., Woods, R.E.: Digital Image Processing, vol. 5, pp. 11–15. Addison-Wesley, Boston (1992) Gonzalez, R.C., Woods, R.E.: Digital Image Processing, vol. 5, pp. 11–15. Addison-Wesley, Boston (1992)
11.
go back to reference Dubochet, J., Frank, J., Henderson, R.: Nobel Prize in Chemistry (2017) Dubochet, J., Frank, J., Henderson, R.: Nobel Prize in Chemistry (2017)
12.
go back to reference Betzig, E., Hell, S.W., Moerner, W.E.: The Nobel Prize in Chemistry (2014) Betzig, E., Hell, S.W., Moerner, W.E.: The Nobel Prize in Chemistry (2014)
15.
go back to reference Nyquist, H.: Certain topics in telegraph transmission theory. Trans. AIEE 47, 363–390 (1928) Nyquist, H.: Certain topics in telegraph transmission theory. Trans. AIEE 47, 363–390 (1928)
16.
go back to reference Kotelnikov, V.A.: On the capacity of the ‘ether’ and cables in electrical communication. In: Proceedings of 1st All-Union Conference on Technological Reconstruction of the Communications Sector and Low-Current Engineering (1933) Kotelnikov, V.A.: On the capacity of the ‘ether’ and cables in electrical communication. In: Proceedings of 1st All-Union Conference on Technological Reconstruction of the Communications Sector and Low-Current Engineering (1933)
Metadata
Title
Unsupervised Parametrization of Nano-Objects in Electron Microscopy
Authors
Pavla Urbanová
Norbert Cyran
Pavel Souček
Antonín Bárta
Vladyslav Bozhynov
Dinara Bekkhozhayeva
Petr Císař
Miloš Železný
Copyright Year
2018
DOI
https://doi.org/10.1007/978-3-319-78723-7_12

Premium Partner