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LAURDAN Fluorescence Properties in Membranes: A Journey from the Fluorometer to the Microscope Read chapter Read first chapter

2013 | OriginalPaper | Chapter

3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: A Toolkit for Biomembrane Research

Authors : Andrey S. Klymchenko, Guy Duportail, Yves Mély

Published in: Fluorescent Methods to Study Biological Membranes

Publisher: Springer Berlin Heidelberg

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Abstract

Environment-sensitive dyes due to the sensitivity of their spectra to the physicochemical properties of their environment are unique tools for probing model and biological membranes. Here, we describe a particular class of environment-sensitive dyes based on 3-hydroxychromones. These dyes exhibit excited-state intramolecular proton transfer resulting in dual emission, highly sensitive to environment polarity and hydration. Appropriate molecular design of the new probes allows precise localization and orientation of their fluorophore in the lipid bilayers, which confer high specificity to particular membrane properties. In this respect, interface localization of the probes allows monitoring lipid order, while vertical orientation is required to achieve sensitivity to dipole and transmembrane potentials. Finally, biological applications of these probes for sensing lipid domains (rafts) and apoptosis are shown.

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previous chapter Application of NBD-Labeled Lipids in Membrane and Cell Biology
next chapter Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy
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Metadata
Title
3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: A Toolkit for Biomembrane Research
Authors
Andrey S. Klymchenko
Guy Duportail
Yves Mély
Copyright Year
2013
Publisher
Springer Berlin Heidelberg
Book
Fluorescent Methods to Study Biological Membranes

Print ISBN: 978-3-642-33127-5

Electronic ISBN: 978-3-642-33128-2

Copyright Year: 2013

DOI
https://doi.org/10.1007/4243_2012_44

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