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2021 | OriginalPaper | Chapter

10. Advanced Fermentation Strategies to Enhance Lipid Production from Lignocellulosic Biomass

Authors : Qiang Fei, Yunyun Liu, Haritha Meruvu, Ziyue Jiao, Rongzhan Fu

Published in: Emerging Technologies for Biorefineries, Biofuels, and Value-Added Commodities

Publisher: Springer International Publishing

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Abstract

This chapter discusses the related researches on microbial lipid biosynthetic processes, the inhibitor tolerance of lipid-producing microorganisms, and high cell density culture strategies for lipid production from lignocellulosic biomass. The aspects covered here mainly focused on the elucidation of lipid accumulations of oleaginous microorganisms in different fermentation modes including batch, fed-batch, and continuous cultivation coupled with multistage strategies for increasing cell densities of oleaginous microbes thereby improving lipid yield, titer, and productivity. Furthermore, because of the inhibitors generated during hydrolysis processes as by-products that influence the lipid biosynthesis, the strategies to enhance the lipid content through metabolic engineering approach including blocking of competing pathways and multigene methods were discussed in this chapter. It is suggested that the efficiency of the lignocellulosic lipid-based biorefinery process would be greatly improved if the cultivation platform of oleaginous microorganisms could integrate both micro-manipulations for the gene expression and fermentation strategies with the online control-feedback system.

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previous chapter Metabolic Engineering of Yeast for Enhanced Natural and Exotic Fatty Acid Production

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Kosa, M., & Ragauskas, A. J. (2011). Lipids from heterotrophic microbes: Advances in metabolism research. Trends in Biotechnology, 29(2), 53–61.CrossRef

Ratledge, C. (2004). Fatty acid biosynthesis in microorganisms being used for single cell oil production. Biochimie, 86(11), 807–815.CrossRef

Garay, L. A., Boundy-Mills, K. L., & German, J. B. (2014). Accumulation of high-value lipids in single-cell microorganisms: A mechanistic approach and future perspectives. Journal of Agricultural and Food Chemistry, 62(13), 2709–2727.CrossRef

Zhang, H., Wu, C., Wu, Q., Dai, J., & Song, Y. (2016). Metabolic flux analysis of lipid biosynthesis in the yeast Yarrowia lipolytica using 13C-labeled glucose and gas chromatography-mass spectrometry. PLoS One, 11(7), e0159187.CrossRef

Pereira, G., Finco, A. M., Letti, L., Karp, S., Pagnoncelli, M., Oliveira, J., Thomaz-Soccol, V., Brar, S., & Soccol, C. (2018). Microbial metabolic pathways in the production of valued-added products. In S. K. Brar, R. K. Das, & S. J. Sarma (Eds.), Microbial sensing in fermentation (pp. 137–167). Hoboken: Wiley.CrossRef

Beopoulos, A., Nicaud, J.-M., & Gaillardin, C. (2011). An overview of lipid metabolism in yeasts and its impact on biotechnological processes. Applied Microbiology and Biotechnology, 90(4), 1193–1206.CrossRef

Ratledge, C., & Wynn, J. P. (2002). The biochemistry and molecular biology of lipid accumulation in oleaginous microorganisms. In A. I. Laskin, J. W. Bennett, & G. M. Gadd (Eds.), Advances in applied microbiology (pp. 1–52). Amsterdam: Academic Press.

Passoth, V. (2017). Lipids of yeasts and filamentous fungi and their importance for biotechnology. In A. A. Sibirny (Ed.), Biotechnology of yeasts and filamentous fungi (pp. 149–204). Cham: Springer International Publishing.CrossRef

Davis, M. S. S. J., & Cronan, J. E., Jr. (2000). Overproduction of acetyl-CoA carboxylase activity increases the rate of fatty acid biosynthesis in Escherichia coli. Journal of Biological Chemistry, 275(37), 28593–28598.CrossRef

10.

Liang, M. H. J. J. G. (2013). Advancing oleaginous microorganisms to produce lipid via metabolic engineering technology. Progress in Lipid Research, 52(4), 395–408.CrossRef

11.

Metz, J. G., Roessler, P., Facciotti, D., Levering, C., Dittrich, F., Lassner, M., Valentine, R., Lardizabal, K., Domergue, F., Yamada, A., Yazawa, K., Knauf, V., & Browse, J. (2001). Production of polyunsaturated fatty acids by polyketide synthases in both prokaryotes and eukaryotes. Science, 293(5528), 290–293.CrossRef

12.

Ferguson, J. J. A., Dias, C. B., & Garg, M. L. (2016). Omega-3 polyunsaturated fatty acids and hyperlipidaemias. In M. V. Hegde, A. A. Zanwar, & S. P. Adekar (Eds.), Omega-3 fatty acids: Keys to nutritional health (pp. 67–78). Cham: Springer International Publishing.CrossRef

13.

Ouyang, L.-L., Chen, S.-H., Li, Y., & Zhou, Z.-G. (2013). Transcriptome analysis reveals unique C4-like photosynthesis and oil body formation in an arachidonic acid-rich microalga Myrmecia incisa Reisigl H4301. BMC Genomics, 14(1), 396.CrossRef

14.

Ma Y.-L. (2006). Microbial oils and its research advance. Chinese Journal of Bioprocess Engineering, 4(4), 7–11.

15.

Athenstaedt, K. D., & G. (1999). Phosphatidic acid, a key intermediate in lipid metabolism. European Journal of Biochemistry, 266(1), 1–16.CrossRef

16.

Coleman, R. A., & Lee, D. P. (2004). Enzymes of triacylglycerol synthesis and their regulation. Progress in Lipid Research, 43(2), 134–176.CrossRef

17.

Galán, B., Santos-Merino, M., Nogales, J., de la Cruz, F., & García, J. L. (2019). Microbial oils as nutraceuticals and animal feeds. In H. Goldfine (Ed.), Health consequences of microbial interactions with hydrocarbons, oils, and lipids (pp. 1–45). Cham: Springer International Publishing.

18.

Minskoff, S. A., Racenis, P. V., Granger, J., Larkins, L., Hajra, A. K., & Greenberg, M. L. (1994). Regulation of phosphatidic acid biosynthetic enzymes in Saccharomyces cerevisiae. Journal of Lipid Research (USA), 35(12), 2254–2262.CrossRef

19.

Liang, M.-H., & Jiang, J.-G. (2013). Advancing oleaginous microorganisms to produce lipid via metabolic engineering technology. Progress in Lipid Research, 52(4), 395–408.CrossRef

20.

Jin, M., Slininger, P. J., Dien, B. S., Waghmode, S., Moser, B. R., Orjuela, A., Sousa, L. C., & Balan, V. (2015). Microbial lipid-based lignocellulosic biorefinery: Feasibility and challenges. Trends in Biotechnology, 33(1), 43–54.CrossRef

21.

Sousa, F. P., Silva, L. N., de Rezende, D. B., de Oliveira, L. C. A., & Pasa, V. M. D. (2018). Simultaneous deoxygenation, cracking and isomerization of palm kernel oil and palm olein over beta zeolite to produce biogasoline, green diesel and biojet-fuel. Fuel, 223, 149–156.CrossRef

22.

Huang, W.-D., & Zhang, Y. H. P. (2011). Analysis of biofuels production from sugar based on three criteria: Thermodynamics, bioenergetics, and product separation. Energy & Environmental Science, 4(3), 784–792.CrossRef

23.

Chang, Y.-H., Chang, K.-S., Lee, C.-F., Hsu, C.-L., Huang, C.-W., & Jang, H.-D. (2015). Microbial lipid production by oleaginous yeast Cryptococcus sp. in the batch cultures using corncob hydrolysate as carbon source. Biomass and Bioenergy, 72, 95–103.CrossRef

24.

Anschau, A., Xavier, M. C. A., Hernalsteens, S., & Franco, T. T. (2014). Effect of feeding strategies on lipid production by Lipomyces starkeyi. Bioresource Technology, 157, 214–222.CrossRef

25.

Fei, Q., O’Brien, M., Nelson, R., Chen, X., Lowell, A., & Dowe, N. (2016). Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source. Biotechnology for Biofuels, 9(1), 1–12.CrossRef

26.

Economou, C. N., Aggelis, G., Pavlou, S., & Vayenas, D. V. (2011). Single cell oil production from rice hulls hydrolysate. Bioresource Technology, 102(20), 9737–9742.CrossRef

27.

Moreton, R. S. (1988). Physiology of lipid accumulation yeast. In R. S. Moreton (Ed.), Single cell oil. London: Longman Higher Education Division.

28.

Zhao, X., Hu, C., Wu, S., Shen, H., & Zhao, Z. K. (2011). Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies. Journal of Industrial Microbiology & Biotechnology, 38(5), 627–632.CrossRef

29.

Hernández-Beltrán, J. U., & Hernández-Escoto, H. (2018). Enzymatic hydrolysis of biomass at high-solids loadings through fed-batch operation. Biomass and Bioenergy, 119, 191–197.CrossRef

30.

Li, Y., Zhao, Z., & Bai, F. (2007). High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture. Enzyme and Microbial Technology, 41(3), 312–317.CrossRef

31.

Liu, Y., Wang, Y., Liu, H., & Zhang, J. a. (2015). Enhanced lipid production with undetoxified corncob hydrolysate by Rhodotorula glutinis using a high cell density culture strategy. Bioresource Technology, 180, 32–39.CrossRef

32.

Kim, B. S., Lee, S. C., Lee, S. Y., Chang, H. N., Chang, Y. K., & Woo, S. I. (1994). Production of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnology and Bioengineering, 43(9), 892.CrossRef

33.

Salehmin, M. N. I., Annuar, M. S. M., & Chisti, Y. (2013). High cell density fed-batch fermentations for lipase production: Feeding strategies and oxygen transfer. Bioprocess and Biosystems Engineering, 36(11), 1527–1543.CrossRef

34.

Hassan, M., Blanc, P. J., Granger, L. M., Pareilleux, A., & Goma, G. (1996). Influence of nitrogen and iron limitations on lipid production by Cryptococcus curvatus grown in batch and fed-batch culture. Process Biochemistry, 31(4), 355–361.CrossRef

35.

Wiebe, M. G., Koivuranta, K., Penttilä, M., & Ruohonen, L. (2012). Lipid production in batch and fed-batch cultures of Rhodosporidium toruloides from 5 and 6 carbon carbohydrates. BMC Biotechnology, 12(1), 26.CrossRef

36.

Slininger, P. J., Dien, B. S., Kurtzman, C. P., Moser, B. R., Bakota, E. L., Thompson, S. R., O’Bryan, P. J., Cotta, M. A., Balan, V., Jin, M., Sousa, L. C., & Dale, B. E. (2016). Comparative lipid production by oleaginous yeasts in hydrolyzates of lignocellulosic biomass and process strategy for high titers. Biotechnology and Bioengineering, 113(8), 1676–1690.CrossRef

37.

Wang, T., Tian, X., Liu, T., Wang, Z., Guan, W., Guo, M., Chu, J., & Zhuang, Y. (2017). A two-stage fed-batch heterotrophic culture of Chlorella protothecoides that combined nitrogen depletion with hyperosmotic stress strategy enhanced lipid yield and productivity. Process Biochemistry, 60, 74–83.CrossRef

38.

Fei, Q., Wewetzer, S. J., Kurosawa, K., Rha, C., & Sinskey, A. J. (2015). High-cell-density cultivation of an engineered Rhodococcus opacus strain for lipid production via co-fermentation of glucose and xylose. Process Biochemistry, 50(4), 500–506.CrossRef

39.

Fu, R., Fei, Q., Shang, L., Brigham, C. J., & Chang, H. N. (2018). Enhanced microbial lipid production by Cryptococcus albidus in the high-cell-density continuous cultivation with membrane cell recycling and two-stage nutrient limitation. Journal of Industrial Microbiology & Biotechnology, 45(12), 1045–1051.CrossRef

40.

Karamerou, E. E., Theodoropoulos, C., & Webb, C. (2017). Evaluating feeding strategies for microbial oil production from glycerol by Rhodotorula glutinis. Engineering in Life Sciences, 17(3), 314–324.CrossRef

41.

Chang, H. N., Kim, N.-J., Kang, J., Jeong, C. M., J-d-r, C., Fei, Q., Kim, B. J., Kwon, S., Lee, S. Y., & Kim, J. (2011). Multi-stage high cell continuous fermentation for high productivity and titer. Bioprocess and Biosystems Engineering, 34(4), 419–431.CrossRef

42.

Chang, H. N., Jung, K., Choi, J.-d.-r., Lee, J. C., & Woo, H.-C. (2014). Multi-stage continuous high cell density culture systems: A review. Biotechnology Advances, 32(2), 514–525.CrossRef

43.

Chang, H. N. F. Q., Choi, J. D. R., & Jung, K. S. (2011b). Economic evaluation of heterotropic microbial lipid (C. albidus) production using low-cost volatile fatty acids in MSC-HCDC bioreactor system. BIT’s first annual congress of bioenergy, pp 0425–0429.

44.

Fei, Q., Chang, H. N., Shang, L., J-d-r, C., Kim, N., & Kang, J. (2011). The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production. Bioresource Technology, 102(3), 2695–2701.CrossRef

45.

Palmqvist, E., & Hahn-Hägerdal, B. (2000). Fermentation of lignocellulosic hydrolysates. II: Inhibitors and mechanisms of inhibition. Bioresource Technology, 74(1), 25–33.CrossRef

46.

Hu, C., Zhao, X., Zhao, J., Wu, S., & Zhao, Z. K. (2009). Effects of biomass hydrolysis by-products on oleaginous yeast Rhodosporidium toruloides. Bioresource Technology, 100(20), 4843–4847.CrossRef

47.

Chao, H., Hong, W., Li-ping, L., Wen-yong, L., & Min-hua, Z. (2012). Effects of alcohol compounds on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans. PLoS One, 7(10), 1–12.

48.

Wang, J., Gao, Q., Zhang, H., & Bao, J. (2016). Inhibitor degradation and lipid accumulation potentials of oleaginous yeast Trichosporon cutaneum using lignocellulose feedstock. Bioresource Technology, 218, 892–901.CrossRef

49.

Huang, C., Wu, H., Smith, T. J., Z-j, L., Lou, W.-Y., & M-h, Z. (2012). In vivo detoxification of furfural during lipid production by the oleaginous yeast Trichosporon fermentans. Biotechnology Letters, 34(9), 1637–1642.CrossRef

50.

Gong, Z., Zhou, W., Shen, H., Yang, Z., Wang, G., Zuo, Z., Hou, Y., & Zhao, Z. K. (2016). Co-fermentation of acetate and sugars facilitating microbial lipid production on acetate-rich biomass hydrolysates. Bioresource Technology, 207, 102–108.CrossRef

51.

Sitepu, I., Selby, T., Lin, T., Zhu, S., & Boundy-Mills, K. (2014). Carbon source utilization and inhibitor tolerance of 45 oleaginous yeast species. Journal of Industrial Microbiology & Biotechnology, 41(7), 1061–1070.CrossRef

52.

Liu, J., Pei, G., Diao, J., Chen, Z., Liu, L., Chen, L., & Zhang, W. (2017). Screening and transcriptomic analysis of Crypthecodinium cohnii mutants with high growth and lipid content using the acetyl-CoA carboxylase inhibitor sethoxydim. Applied Microbiology and Biotechnology, 101(15), 6179–6191.CrossRef

53.

Cao, S., Zhou, X., Jin, W., Wang, F., Tu, R., Han, S., Chen, H., Chen, C., Xie, G.-J., & Ma, F. (2017). Improving of lipid productivity of the oleaginous microalgae Chlorella pyrenoidosa via atmospheric and room temperature plasma (ARTP). Bioresource Technology, 244, 1400–1406.CrossRef

54.

Sun, X., Li, P., Liu, X., Wang, X., Liu, Y., Turaib, A., & Cheng, Z. (2020). Strategies for enhanced lipid production of Desmodesmus sp. mutated by atmospheric and room temperature plasma with a new efficient screening method. Journal of Cleaner Production, 250, 119509.CrossRef

55.

Tai, M., & Stephanopoulos, G. (2013). Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production. Metabolic Engineering, 15, 1–9.CrossRef

56.

Kamisaka, Y., Kimura, K., Uemura, H., & Yamaoka, M. (2013). Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast. Applied Microbiology and Biotechnology, 97(16), 7345–7355.CrossRef

57.

Ledesma-Amaro, R., Santos, M. A., Jiménez, A., & Revuelta, J. L. (2014). Strain design of Ashbya gossypii for single-cell oil production. Applied and Environmental Microbiology, 80(4), 1237–1244.CrossRef

58.

Kurosawa, K., Wewetzer, S. J., & Sinskey, A. J. (2013). Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Biotechnology for Biofuels, 6(1), 134.CrossRef

59.

Kurosawa, K., Laser, J., & Sinskey, A. J. (2015). Tolerance and adaptive evolution of triacylglycerol-producing Rhodococcus opacus to lignocellulose-derived inhibitors. Biotechnology for Biofuels, 8, 76–85.CrossRef

60.

Zhang, Y. W. L. W. (2012). Advances in the research of microalgae bioenergy. Marine Sciences, 36, 132–138.

61.

Majidian, P., Tabatabaei, M., Zeinolabedini, M., Naghshbandi, M. P., & Chisti, Y. (2018). Metabolic engineering of microorganisms for biofuel production. Renewable and Sustainable Energy Reviews, 82, 3863–3885.CrossRef

62.

Li, Y., Han, D., Hu, G., Dauvillee, D., Sommerfeld, M., Ball, S., & Hu, Q. (2010). Chlamydomonas starchless mutant defective in ADP-glucose pyrophosphorylase hyper-accumulates triacylglycerol. Metabolic Engineering, 12(4), 387–391.CrossRef

63.

Sahay, S., & Braganza, V. J. (2016). Microalgae based biodiesel production-current and future scenario. Journal of Experimental Science, 7, 31–35.CrossRef

64.

Beopoulos, A., Mrozova, Z., Thevenieau, F., Le Dall, M.-T., Hapala, I., Papanikolaou, S., Chardot, T., & Nicaud, J.-M. (2008). Control of lipid accumulation in the yeast Yarrowia lipolytica. Applied and Environmental Microbiology, 74(24), 7779–7789.CrossRef

65.

Kalscheuer, R., Stölting, T., & Steinbüchel, A. (2006). Microdiesel: Escherichia coli engineered for fuel production. Microbiology, 152(9), 2529–2536.CrossRef

Title: Advanced Fermentation Strategies to Enhance Lipid Production from Lignocellulosic Biomass
Authors: Qiang Fei
Yunyun Liu
Haritha Meruvu
Ziyue Jiao
Rongzhan Fu
Publisher: Springer International Publishing
Book: Emerging Technologies for Biorefineries, Biofuels, and Value-Added Commodities
Print ISBN: 978-3-030-65583-9

Electronic ISBN: 978-3-030-65584-6

Copyright Year: 2021
DOI: https://doi.org/10.1007/978-3-030-65584-6_10