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Biomass Conversion and Biorefinery

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11-09-2023 | Original Article

An eco-friendly EnZolv pretreatment optimization in banana fiber biomass using response surface methodology (RSM) and its structural characterization

Authors: Oviya Govindaraj, Gopal Nellaiappan Olaganathan, Raja ASM, Sivakumar Uthandi

Published in: Biomass Conversion and Biorefinery | Issue 1/2024

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Abstract

An EnZolv is a recently developed novel pretreatment technique used for efficient delignification in lignocellulosic biomass and recognized as a greener approach. Mostly, banana fiber was pretreated by chemical process which causes environmental pollution and for the first time, we report here EnZolv-based banana fiber biomass pretreatment and its optimization using response surface methodology (RSM). Thus, the present study aimed to optimize the influential parameters in EnZolv process for banana fiber biomass delignification. Pre-optimization studies resulted in an increased cellulose recovery and lignin reduction when banana fiber was pretreated at 100% moisture content, incubated at 40 °C temperature, with enzyme load of 50 U per gram of biomass for an incubation time of 5 h at a shaking speed of 100 rpm. Based on pre-optimization, the EnZolv pretreatment process for banana fiber was optimized using RSM in Box-Behnken design which revealed enhanced cellulose recovery (74.7%) at 100% initial moisture content of substrate using laccase enzyme load of 50 U g⁻¹ of biomass, and incubation time of 5.7 h at a temperature of 40 °C and shaking at 100 rpm. Biophysical and structural analysis of treated biomass revealed a reduction in lignin peak intensity at lignin region in FTIR spectrum, deconstruction of biomass as visualized in scanning electron microscope image, increased crystallinity (62.7%) of biomass by XRD analyses, and thermal stability of pretreated biomass analyzed by TGA. The EnZolv process generated industrially important high-value compounds like vanillin, benzaldehyde, hydroquinone, hydroxytoluene, and benzoic acid during the delignification process. All the biophysical techniques and generated lignin-derived compounds validated the efficiency of the optimized EnZolv conditions in banana fiber biomass. Thus, the result from the manuscript will be highly useful for eco-friendly pretreatment-based research on lignocellulosic biomass.

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Title: An eco-friendly EnZolv pretreatment optimization in banana fiber biomass using response surface methodology (RSM) and its structural characterization
Authors: Oviya Govindaraj
Gopal Nellaiappan Olaganathan
Raja ASM
Sivakumar Uthandi
Publication date: 11-09-2023
Publisher: Springer Berlin Heidelberg
Published in: Biomass Conversion and Biorefinery / Issue 1/2024
Print ISSN: 2190-6815
Electronic ISSN: 2190-6823
DOI: https://doi.org/10.1007/s13399-023-04829-y

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