Skip to main content
Disciplines
Automotive Business IT + Informatics Construction + Real Estate Electrical Engineering + Electronics Energy + Sustainability Insurance + Risk Finance + Banking Management + Leadership Marketing + Sales Mechanical Engineering + Materials
Events
DE
EN
Books
Journals
Topic Page
Marketing
Start single access now
Access for companies
EXTENDED SEARCH
Log in
Top
Cellulose
Published in: Cellulose 11/2017

30-08-2017 | Original Paper

Bacterial cellulose/gelatin scaffold loaded with VEGF-silk fibroin nanoparticles for improving angiogenesis in tissue regeneration

Authors: Baoxiu Wang, Xiangguo Lv, Shiyan Chen, Zhe Li, Jingjing Yao, Xufeng Peng, Chao Feng, Yuemin Xu, Huaping Wang

Published in: Cellulose | Issue 11/2017

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Due to its unique properties, bacterial cellulose (BC) has attracted a great deal of interest as an implant material for tissue regeneration. However, one major problem of BC is inadequate vascularization which leads to cell apoptosis due to insufficient nutrients and oxygen supply. Herein, porous BC/gelatin (BC/Gel) scaffolds loaded with vascular endothelial growth factor (VEGF) with silk fibroin nanoparticles (VEGF-NPs) were prepared. An in vitro study indicated that VEGF was sustainably released from the BC/Gel/VEGF-NPs scaffold over 28 days. Cell viability, morphology and proliferation were evaluated using Live/Dead® viability/cytotoxicity assay, field emission scanning electron microscopy and CCK-8 assay by seeding the scaffolds with pig iliac endothelium cells. The presence of VEGF-NPs in the scaffold significantly improved cell proliferation and viability in vitro. Evaluation of in vivo biocompatibility and angiogenesis of the BC/Gel/VEGF-NPs scaffold was conducted using a dog skin defect model. Results indicated that the BC/Gel/VEGF-NPs scaffold significantly promoted vessel blood formation after implantation compared to the BC/Gel and BC/Gel/NPs scaffolds. It is concluded that angiogenesis could be improved through the incorporation of VEGF-NPs into the BC/Gel scaffold, which may enhance clinically desirable functions of BC-based scaffolds in terms of enhanced angiogenesis.
previous article Silver nanoparticles supported on polyethylene glycol/cellulose acetate ultrafiltration membranes: preparation and characterization of composite
next article Microwave-assisted facile synthesis of TEMPO-oxidized cellulose beads with high adsorption capacity for organic dyes

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now
Literature
Alidadi S, Oryan A, Bigham-Sadegh A, Moshiri A (2017) Role of platelet gel embedded within gelatin scaffold on healing of experimentally induced critical-sized radial bone defects in rats. Int Orthop 41:805–812CrossRef
Berti FV, Rambo CR, Dias PF, Porto LM (2013) Nanofiber density determines endothelial cell behavior on hydrogel matrix. Mater Sci Eng C 33:4684–4691CrossRef
Bessa PC et al (2010) Silk fibroin microparticles as carriers for delivery of human recombinant BMPs. Physical characterization and drug release. J Tissue Eng Regen Med 4:349–355CrossRef
Bhardwaj N, Singh YP, Devi D, Kandimalla R, Kotoky J, Mandal BB (2016) Potential of silk fibroin/chondrocyte constructs of muga silkworm Antheraea assamensis for cartilage tissue engineering. J Mater Chem B 4:3670–3684CrossRef
Bodin A, Ahrenstedt L, Fink H, Brumer H, Risberg B, Gatenholm P (2007) Modification of nanocellulose with a xyloglucan-RGD conjugate enhances adhesion and proliferation of endothelial cells: implications for tissue engineering. Biomacromol 8:3697–3704CrossRef
Cao Z, Chen X, Yao J, Huang L, Shao Z (2007) The preparation of regenerated silk fibroin microspheres. Soft Matter 3:910–915CrossRef
Carenza E et al (2015) Encapsulation of VEGF(165) into magnetic PLGA nanocapsules for potential local delivery and bioactivity in human brain endothelial cells. J Mater Chem B 3:2538–2544CrossRef
Chu H, Gao J, Chen C-W, Huard J, Wang Y (2011) Injectable fibroblast growth factor-2 coacervate for persistent angiogenesis. Proc Natl Acad Sci 108:13444–13449CrossRef
Dai W, Guo H, Qian D, Qin Z, Lei Y, Hou X (2016) Improving endothelialization by combined application of polyethylene glycol coated cerium oxide nanoparticles and VEGF in electrospun polyurethane scaffolds. J Mater Chem B 5:1053–1061CrossRef
Favi PM, Ospina SP, Kachole M, Gao M, Atehortua L, Webster TJ (2016) Preparation and characterization of biodegradable nano hydroxyapatite–bacterial cellulose composites with well-defined honeycomb pore arrays for bone tissue engineering applications. Cellulose 23:1263–1282CrossRef
Ferrara N (2009) VEGF-A: a critical regulator of blood vessel growth. Eur Cytokine Netw 20:158–163
Ferrara N, Gerber H-P, LeCouter J (2003) The biology of VEGF and its receptors. Nat Med 9:669–676CrossRef
Geng H, Song H, Qi J, Cui D (2011) Sustained release of VEGF from PLGA nanoparticles embedded thermo-sensitive hydrogel in full-thickness porcine bladder acellular matrix. Nanoscale Res Lett 6:1–8CrossRef
Guan J, Stankus JJ, Wagner WR (2007) Biodegradable elastomeric scaffolds with basic fibroblast growth factor release. J Control Release 120:70–78CrossRef
He Q et al (2011) Improved cellularization and angiogenesis using collagen scaffolds chemically conjugated with vascular endothelial growth factor. Acta Biomater 7:1084–1093CrossRef
Ho Y-C, Mi F-L, Sung H-W, Kuo P-L (2009) Heparin-functionalized chitosan-alginate scaffolds for controlled release of growth factor. Int J Pharm 376:69–75CrossRef
Huang JW et al (2015) Urethral reconstruction with a 3D porous bacterial cellulose scaffold seeded with lingual keratinocytes in a rabbit model. Biomed Mater 10:055005–055013CrossRef
Inzana JA et al (2014) 3D printing of composite calcium phosphate and collagen scaffolds for bone regeneration. Biomaterials 35:4026–4034CrossRef
Kucińska-Lipka J, Gubanska I, Janik H (2015) Bacterial cellulose in the field of wound healing and regenerative medicine of skin: recent trends and future prospectives. Polym Bull 72:2399–2419CrossRef
Kundu J, Chung Y-I, Kim YH, Tae G, Kundu S (2010) Silk fibroin nanoparticles for cellular uptake and control release. Int J Pharm 388:242–250CrossRef
Laschke MW et al (2006) Angiogenesis in tissue engineering: breathing life into constructed tissue substitutes. Tissue Eng 12:2093–2104CrossRef
Li Z et al (2015) Bladder acellular matrix graft reinforced silk fibroin composite scaffolds loaded VEGF with aligned electrospun fFibers in multiple layers. ACS Biomater Sci Eng 1:238–246CrossRef
Li Z, Lv X, Chen S, Wang B, Feng C, Xu Y, Wang H (2016) Improved cell infiltration and vascularization of three-dimensional bacterial cellulose nanofibrous scaffolds by template biosynthesis. RSC Adv 6:42229–42239CrossRef
Lovett M, Lee K, Edwards A, Kaplan DL (2009) Vascularization strategies for tissue engineering. Tissue Eng Part B 15:353–370CrossRef
Lv X et al (2015) Bacterial cellulose-based biomimetic nanofibrous scaffold with muscle cells for hollow organ tissue engineering. ACS Biomater Sci Eng 2:19–29CrossRef
Magalhães PO, Lopes AM, Mazzola PG, Rangel-Yagui C, Penna T, Pessoa A Jr (2007) Methods of endotoxin removal from biological preparations: a review. J Pharm Pharm Sci 10:388–404
Mohandas A, Anisha B, Chennazhi K, Jayakumar R (2015) Chitosan-hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds. Colloids Surf B Biointerfaces 127:105–113CrossRef
Nakamura S, Kubo T, Ijima H (2013) Heparin-conjugated gelatin as a growth factor immobilization scaffold. J Biosci Bioeng 115:562–567CrossRef
Novotna K et al (2013) Cellulose-based materials as scaffolds for tissue engineering. Cellulose 20:2263–2278CrossRef
Park S et al (2015) In situ hybridization of carbon nanotubes with bacterial cellulose for three-dimensional hybrid bioscaffolds. Biomaterials 58:93–102CrossRef
Petersen N, Gatenholm P (2011) Bacterial cellulose-based materials and medical devices: current state and perspectives. Appl Microbiol Biotechnol 91:1277CrossRef
Place ES, Evans ND, Stevens MM (2009) Complexity in biomaterials for tissue engineering. Nat Mater 8:457–470CrossRef
Rnjak-Kovacina J, Wray LS, Burke KA, Torregrosa T, Golinski JM, Huang W, Kaplan DL (2015) Lyophilized silk sponges: a versatile biomaterial platform for soft tissue engineering. ACS Biomater Sci Eng 1:260–270CrossRef
Shen YH, Shoichet MS, Radisic M (2008) Vascular endothelial growth factor immobilized in collagen scaffold promotes penetration and proliferation of endothelial cells. Acta Biomater 4:477–489CrossRef
Shevchenko RV et al (2014) The in vitro characterization of a gelatin scaffold, prepared by cryogelation and assessed in vivo as a dermal replacement in wound repair. Acta Biomater 10:3156–3166CrossRef
Shi W et al (2017) Structurally and functionally optimized silk-fibroin–gelatin scaffold using 3D printing to repair cartilageinjuryin vitro and in vivo. Adv Mater 29:1701089–1701095CrossRef
Tabata Y (2003) Tissue regeneration based on growth factor release. Tissue Eng 9:5–15CrossRef
Tan Q, Tang H, Hu J, Hu Y, Zhou X, Tao Y, Wu Z (2011) Controlled release of chitosan/heparin nanoparticle-delivered VEGF enhances regeneration of decellularized tissue-engineered scaffolds. Int J Nanomed 6:929–942CrossRef
Tian T, Han Y, Ma B, Wu C, Chang J (2015) Novel Co-akermanite (Ca2CoSi2O7) bioceramics with the activity to stimulate osteogenesis and angiogenesis. J Mater Chem B 3:6773–6782CrossRef
Uematsu K et al (2005) Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold. Biomaterials 26:4273–4279CrossRef
Xiao L, Lu G, Lu Q, Kaplan DL (2016) Direct formation of silk nanoparticles for drug delivery. ACS Biomater Sci Eng 2:2050–2057CrossRef
Yin N, Stilwell MD, Santos TM, Wang H, Weibel DB (2015) Agarose particle-templated porous bacterial cellulose and its application in cartilage growth in vitro. Acta Biomater 12:129–138CrossRef
Zhou J et al (2016) Promotion of adhesion and proliferation of endothelial progenitor cells on decellularized valves by covalent incorporation of RGD peptide and VEGF. J Mater Sci Mater Med 27:142–154CrossRef
Zieris A, Prokoph S, Levental KR, Welzel PB, Grimmer M, Freudenberg U, Werner C (2010) FGF-2 and VEGF functionalization of starPEG-heparin hydrogels to modulate biomolecular and physical cues of angiogenesis. Biomaterials 31:7985–7994CrossRef
Metadata
Title
Bacterial cellulose/gelatin scaffold loaded with VEGF-silk fibroin nanoparticles for improving angiogenesis in tissue regeneration
Authors
Baoxiu Wang
Xiangguo Lv
Shiyan Chen
Zhe Li
Jingjing Yao
Xufeng Peng
Chao Feng
Yuemin Xu
Huaping Wang
Publication date
30-08-2017
Publisher
Springer Netherlands
Published in
Cellulose / Issue 11/2017
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-017-1472-x

Other articles of this Issue 11/2017

Cellulose 11/2017 Go to the issue

Original Paper

Comparative characteristics of TEMPO-oxidized cellulose nanofibers and resulting nanopapers from bamboo, softwood, and hardwood pulps

Original Paper

Microwave-assisted facile synthesis of TEMPO-oxidized cellulose beads with high adsorption capacity for organic dyes

Original Paper

Silver nanoparticles supported on polyethylene glycol/cellulose acetate ultrafiltration membranes: preparation and characterization of composite

Original Paper

Enhanced dispersion and interface compatibilization of crystalline nanocellulose in polylactide by surfactant adsorption

Original Paper

Modified nanoporous magnetic cellulose–chitosan microspheres for efficient removal of Pb(II) and methylene blue from aqueous solution

Original Paper

Cellulose laurate ester aerogel as a novel absorbing material for removing pollutants from organic wastewater