Top

Cellulose

Published in:

01-02-2014 | Original Paper

Characterization of sugarcane bagasse by autofluorescence microscopy

Authors: Tiago A. Chimenez, Marcelo H. Gehlen, Karen Marabezi, Antonio A. S. Curvelo

Published in: Cellulose | Issue 1/2014

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

The spatial distribution of chemical compounds in sugarcane bagasse is an important issue in its use as a raw material for second generation ethanol production from cellulose hydrolysis. Lignocellulosic materials including whole bagasse, fiber, pith, and respective samples obtained after chemical bleaching were investigated using confocal fluorescence microscopy and spectroscopy with one and two-photon excitation. Autofluorescence from unbleached samples revealed that emission from fiber walls containing the lignin fraction was longitudinally oriented. After bleaching treatment, the oriented emission was partially disrupted. Autofluorescence from bleached samples with a residual lignin content of about 1 % was ascribed to improved dispersion of remaining fluorophores throughout the samples inducing a concomitant reduction of fluorescence self-quenching in the samples. The combination of autofluorescence images with spectral emission and lifetime measurements provides a tool for microscopic characterization of natural bagasse samples. Moreover, the technique allows monitoring bleaching processes related to lignin removal.

previous article Sugarcane bagasse cellulose fibres and their hydrous niobium phosphate composites: synthesis and characterization by XPS, XRD and SEM

next article Topical caffeine delivery using biocellulose membranes: a potential innovative system for cellulite treatment

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Aguilar R, Ramirez JA, Garrote G, Vazquez M (2002) Kinetic study of the acid hydrolysis of sugar cane bagasse. J Food Eng 55(4):309–318CrossRef

Albinsson B, Li SM, Lundquist K, Stomberg R (1999) The origin of lignin fluorescence. J Mol Struct 508:19–27CrossRef

Benediktyova Z, Nedbal L (2009) Imaging of multi-color fluorescence emission from leaf tissues. Photosynth Res 102:169–175CrossRef

Billinton N, Knight AW (2001) Seeing the wood through the trees: a review of techniques for distinguishing green fluorescent protein from endogenous autofluorescence. Anal Biochem 291:175–197CrossRef

Bond J, Donaldson L, Hill S, Hitchcock K (2008) Safranine fluorescent staining of wood cell walls. Biotech Histochem 83:161–171CrossRef

Browning BL (1967) Methods on wood chemistry. Interscience Publishers, New York

Callis PR (1993) On the theory of 2-photon induced fluorescence anisotropy with application to indoles. J Chem Phys 99(1):27–37CrossRef

Castellan A, Davidson RS (1994) Steady-state and dynamic fluorescence emission from abies wood. J Photochem Photobiol, A 78:275–279CrossRef

Castellan A, Ruggiero R, Frollini E, Ramos LA, Chirat C (2007) Studies on fluorescence of cellulosics. Holzforschung 61:504–508CrossRef

Cheng PC, Pan SJ, Shih A, Kim KS, Liou WS, Park MS (1998) Highly efficient upconverters for multiphoton fluorescence microscopy. J Microsc Oxf 189:199–212CrossRef

Cheng PC, Lin BL, Kao FJ, Sun CK (2000a) Multi photon fluorescence microscopy: behavior of biological specimens under high intensity illumination. In: Conference on optical sensing, imaging, and manipulation for biological and biomedical applications 4082:134–138, Taipei, Taiwan

Cheng PC, Lin BL, Kao FJ, Sun CK, Wang YS, Liu TM, Wan YM, Chen JC, Huang MK, Johnson I (2000b) Multi-photon fluorescence spectroscopy of fluorescent bio-probes and bio-molecules. In: Conference on optical sensing, imaging, and manipulation for biological and biomedical applications 4082:87–91, Taipei, Taiwan

Cheng PC, Lin BL, Kao FJ, Gu M, Xu MG, Gan XS, Huang MK, Wang YS (2001) Multi-photon fluorescence microscopy—the response of plant cells to high intensity illumination. Micron 32:661–669CrossRef

Coletta VC, Rezende CA, da Conceicao FR, Polikarpov I, Gontijo Guimaraes FE (2013) Mapping the lignin distribution in pretreated sugarcane bagasse by confocal and fluorescence lifetime imaging microscopy. Biotechnol Biofuels 6(43):1–10

Daniel G, Nilsson T, Pettersson B (1991) Poorly and non-lignified regions in the middle lamella cell corners of birch (betula-verrucosa) and other wood species. Iawa Bull 12(1):70–83

Denk W, Strickler JH, Webb WW (1990) 2-photon laser scanning fluorescence microscopy. Science 248:73–76CrossRef

Donaldson L, Radotic K, Kalauzi A, Djikanovic D, Jeremic M (2010) Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. J Struct Biol 169:106–115CrossRef

Driemeier C, Calligaris GA (2011) Theoretical and experimental developments for accurate determination of crystallinity of cellulose I materials. J Appl Crystallogr 44:184–192CrossRef

Driemeier C, Pimenta MTB, Rocha GJM, Oliveira MM, Mello DB, Maziero P, Goncalves AR (2011) Evolution of cellulose crystals during prehydrolysis and soda delignification of sugarcane lignocellulose. Cellulose 18:1509–1519CrossRef

Emsley AM (2008) Cellulosic ethanol re-ignites the fire of cellulose degradation. Cellulose 15:187–192CrossRef

Farrell AE, Plevin RJ, Turner BT, Jones AD, O’Hare M, Kammen DM (2006) Ethanol can contribute to energy and environmental goals. Science 311:506–508CrossRef

Gehlen MH (2009) Approximate solution of the autocatalytic hydrolysis of cellulose. Cellulose 16:1069–1073CrossRef

Gehlen MH (2010) Kinetics of autocatalytic acid hydrolysis of cellulose with crystalline and amorphous fractions. Cellulose 17:245–252CrossRef

Goldemberg J (2007) Ethanol for a sustainable energy future. Science 315:808–810CrossRef

Gray KA (2007) Cellulosic ethanol—state of the technology. Int Sugar J 109(1299):145

Hafren J, Oosterveld-Hut HMJ (2009) Fluorescence lifetime imaging microscopy study of wood fibers. J Wood Sci 55:236–239CrossRef

Harris PJ, Hartley RD (1976) Detection of bound ferulic acid in cell-walls of gramineae by ultraviolet fluorescence microscopy. Nature 259:508–510CrossRef

Harris PJ, Hartley RD (1980) Phenolic constituents of the cell-walls of monocotyledons. Biochem Syst Ecol 8:153–160CrossRef

Harter K, Meixner AJ, Schleifenbaum F (2012) Spectro-microscopy of living plant cells. Mol Plant 5:14–26CrossRef

He LF, Terashima N (1991) Formation and structure of lignin in monocotyledons.4. deposition process and structural diversity of the lignin in the cell-wall of sugarcane and rice plant studied by ultraviolet microscopic spectroscopy. Holzforschung 45:191–198CrossRef

Heskes AM, Lincoln CN, Goodger JQD, Woodrow IE, Smith TA (2012) Multiphoton fluorescence lifetime imaging shows spatial segregation of secondary metabolites in Eucalyptus secretory cavities. J Microsc 247:33–42CrossRef

Horobin R (2002) Conn’s biological stains: a handbook of dyes, stains and fluorochromes for use in biology and medicine, 1st edn. Taylor and Francis, Oxford

Jaedicke K, Roesler J, Gans T, Hughes J (2011) Bellis perennis: a useful tool for protein localization studies. Planta 234:759–768CrossRef

Joseleau JP, Imai T, Kuroda K, Ruel K (2004) Detection in situ and characterization of lignin in the G-layer of tension wood fibres of Populus deltoides. Planta 219:338–345CrossRef

Konig K (2000) Multiphoton microscopy in life sciences. J Microsc Oxf 200:83–104CrossRef

Konya KC, Scaiano JC (1994) Development and applications of pyrene-containing fluorescent-probes for monitoring the photodegradation of lignin-rich products. Chem Mater 6:2369–2375CrossRef

Li Z, Zhai H, Zhang Y, Yu L (2012) Cell morphology and chemical characteristics of corn stover fractions. Ind Crops Prod 37:130–136CrossRef

Nassar MM, Ashour EA, Wahid SS (1996) Thermal characteristics of bagasse. J Appl Polym Sci 61(6):885–890CrossRef

Novaes RC, Teixeira FM, Perrone CC, Sant’Anna C, de Souza W, Abud Y, da Silva Bon EP, Ferreira-Leitao V (2012) Structural evaluation of sugar cane bagasse steam pretreated in the presence of CO₂ and SO₂. Biotechnol Biofuels 5(36):1–8

Olmstead JA, Gray DG (1993) Fluorescence emission from mechanical pulp sheets. J Photochem Photobiol A Chem 73:59–65CrossRef

Olmstead JA, Gray DG (1997) Fluorescence spectroscopy of cellulose, lignin and mechanical pulps: a review. J Pulp Pap Sci 23:J571–J581

Parameswaran N, Liese W (1982) Ultrastructural-localization of wall components in wood cells. Holz Als Roh-Und Werkstoff 40(4):145–155CrossRef

Perera PN, Schmidt M, Schuck PJ, Adams PD (2011) Blind image analysis for the compositional and structural characterization of plant cell walls. Anal Chim Acta 702:172–177CrossRef

Saka S, Goring DAI (1988) The distribution of lignin in white birch wood as determined by bromination with TEM-EDXA. Holzforschung 42(3):149–153CrossRef

Sant’Anna C, Costa LT, Abud Y, Biancatto L, Miguens FC, de Souza W (2013) Sugarcane cell wall structure and lignin distribution investigated by confocal and electron microscopy. Microsc Res Tech 76(8):829–834CrossRef

Schwille P, Haupts U, Maiti S, Webb WW (1999) Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys J 77:2251–2265CrossRef

Ulrich V, Fischer P, Riemann I, Konig K (2004) Compact multiphoton/single photon laser scanning microscope for spectral imaging and fluorescence lifetime imaging. Scanning 26:217–225CrossRef

Vazquez-Cooz I, Meyer RW (2002) A differential staining method to identify lignified and unlignified tissues. Biotech Histochem 77:277–282

Westermark U, Lidbrandt O, Eriksson I (1988) Lignin distribution in spruce (picea-abies) determined by mercurization with SEM-EDXA technique. Wood Sci Technol 22(3):243–250CrossRef

Wheals AE, Basso LC, Alves DMG, Amorim HV (1999) Fuel ethanol after 25 years. Trends Biotechnol 17:482–487CrossRef

Wuyts N, Palauqui J-C, Conejero G, Verdeil J-L, Granier C, Massonnet C (2010) High-contrast three-dimensional imaging of the Arabidopsis leaf enables the analysis of cell dimensions in the epidermis and mesophyll. Plant Methods 6. doi:10.1186/1746-4811-6-17

Zhu P, Moran-Mirabal JM, Luterbacher JS, Walker LP, Craighead HG (2011) Observing Thermobifida fusca cellulase binding to pretreated wood particles using time-lapse confocal laser scanning microscopy. Cellulose 18:749–758CrossRef

Title: Characterization of sugarcane bagasse by autofluorescence microscopy
Authors: Tiago A. Chimenez
Marcelo H. Gehlen
Karen Marabezi
Antonio A. S. Curvelo
Publication date: 01-02-2014
Publisher: Springer Netherlands
Published in: Cellulose / Issue 1/2014
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-013-0135-9

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Springer Professional "Wirtschaft+Technik"

Other articles of this Issue 1/2014

Radial crystalline texture in a lyocell fibre revealed by synchrotron nanofocus wide-angle X-ray scattering

SEC-MALLS analysis of TEMPO-oxidized celluloses using methylation of carboxyl groups

Rheological behavior of cellulose/silk fibroin blend solutions with ionic liquid as solvent

Cellulose scaffolds with an aligned and open porosity fabricated via ice-templating

Utilization of a biphasic oil/aqueous cellulose nanofiber membrane bioreactor with immobilized lipase for continuous hydrolysis of olive oil

Preparation and characterization of cellulose nanofibers from partly mercerized cotton by mixed acid hydrolysis