Top

Cellulose

Published in:

10-11-2021 | Original Research

Cissus quadrangularis stem derived fiber: a natural osteoinductive substrate for regenerative bone tissue engineering

Authors: Praseetha R. Nair, S. Sreeja, G. S. Sailaja

Published in: Cellulose | Issue 1/2022

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Natural biomaterials with intrinsic biomineralization potential are of utmost interest in bone tissue engineering (BTE) applications. Here, we report the biomimetic mineralization potential of a natural fiber derived from Cissus quadrangularis (CQ) stem, CQF, with inherent biomineralization and osteoinductive potential and is proposed for regenerative BTE. CQF has an organized microstructure characterized by an array of submicron size fibrils (SEM analysis) saturated with –OH groups of cellulose on the surface (FTIR spectrum), which provides a better platform for cell adhesion/proliferation and play a key role in the early nucleation of calcium phosphate, the inorganic phase of bone. A single unit of CQF has a tensile strength of 89.75 ± 5.25 MPa, which is in line with the mechanical property requirement of bone. CQF depicts signs of surface erosion (SEM analysis) with a weight loss of 11% after 30 days incubation in physiological conditions, supporting its intended stability required during the bone regeneration phase. CQF demonstrated excellent cytocompatibility (MTT assay and cell adhesion) and elicited primary nucleation and growth of apatite crystals (Ca/P 1.30; day 7), progressed further to secondary growth (Ca/P 1.51; day 14) upon incubation in simulated body fluid (SBF; 1.5X). Biomineralization of CQF was further confirmed by Alizarin Red S staining (day 14) in MG-63 cells. The osteoinductive potential of CQF was validated by quantifying early osteoblast differentiation marker alkaline phosphatase activity in Rat Bone marrow Mesenchymal Cells (BMC). The results, therefore, confer that CQF could be proposed for regenerative BTE.

Graphical abstract

previous article Burst-free and sustained release of diclofenac sodium from mesoporous silica/PEI microspheres coated with carboxymethyl cellulose/chitosan layer-by-layer films

next article Poly ɛ-caprolactone/nanostarch composite nanofibrous wound dressing with antibacterial property and pH stimulus drug release

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Available only for authorised users

Barber AH, Lu D, Pugno NM (2015) Extreme strength observed in limpet teeth. J R Soc Interface 12:5. https://doi.org/10.1098/rsif.2014.1326CrossRef

Bodo M, Lilli C, Bellucci C et al (2002) Basic fibroblast growth factor autocrine loop controls human osteosarcoma phenotyping and differentiation basic fibroblast growth factor autocrine loop controls human osteosarcoma phenotyping and differentiation. J Mol Med 8:393–404. https://doi.org/10.1007/BF03402020CrossRef

Dimitriou R, Jones E, Mc Gonagle D, Giannoudis PV (2011) Bone regeneration: current concepts and future directions. BMC Med 9:66–76CrossRef

Engler AJ, Sen S, Sweeney HL, Discher DE (2006) Matrix elasticity directs stem cell lineage specification. Cell. https://doi.org/10.1016/j.cell.2006.06.044CrossRefPubMed

García-gareta E, Coathup MJ, Blunn GW (2015) Osteoinduction of bone grafting materials for bone repair and regeneration. Bone 81:112–121. https://doi.org/10.1016/j.bone.2015.07.007CrossRefPubMed

Ghasemi T, Shahroudi A, Ebrahimzadeh MH, Mousavian A (2018) Current concepts in scaffolding for bone tissue engineering. Arch Bone Jt Surg. https://doi.org/10.22038/ABJS.2018.26340.1713

Gregory CA, Gunn WG, Peister A, Prockop DJ (2004) An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Anal Biochem 329:77–84. https://doi.org/10.1016/j.ab.2004.02.002CrossRefPubMed

Guerado E, Caso E (2017) Challenges of bone tissue engineering in orthopaedic patients. World J Orthop 8:87. https://doi.org/10.5312/wjo.v8.i2.87CrossRefPubMedPubMedCentral

Indran S, Raj RE (2015) Characterization of new natural cellulosic fiber from Cissus quadrangularis stem. Carbohydr Polym 117:392–399. https://doi.org/10.1016/j.carbpol.2014.09.072CrossRefPubMed

Jayaramudu J, Guduri BR, Rajulu AV (2010) Characterization of new natural cellulosic fabric Grewia tilifolia. Carbohydr Polym 79:847–851. https://doi.org/10.1016/j.carbpol.2009.10.046CrossRef

Jiang T, Nukavarapu SP, Deng M et al (2010) Chitosan-poly(lactide-co-glycolide) microsphere-based scaffolds for bone tissue engineering: In vitro degradation and in vivo bone regeneration studies. Acta Biomater 6:3457–3470. https://doi.org/10.1016/j.actbio.2010.03.023CrossRefPubMed

Johnathan NG, Spiller K, Bernhard J, Novakovic GV (2017) Biomimetic approaches for bone tissue engineering. Tissue Eng Part B 23:480–493. https://doi.org/10.1089/ten.teb.2016.0289CrossRef

Kalita H, Hazarika A, Kandimalla R et al (2018) Development of banana (Musa balbisiana) pseudo stem fiber as a surgical bio-tool to avert post-operative wound infections -. RSC Adv. https://doi.org/10.1039/c8ra04470hCrossRef

Kandimalla R, Kalita S, Choudhury B et al (2016) Fiber from ramie plant ( Boehmeria nivea ): A novel suture biomaterial. Mater Sci Eng C 62:816–822. https://doi.org/10.1016/j.msec.2016.02.040CrossRef

Kerativitayanan P, Tatullo M, Khariton M et al (2017) Nanoengineered osteoinductive and elastomeric scaffolds for bone tissue engineering. ACS Biomater Sci Eng. https://doi.org/10.1021/acsbiomaterials.7b00029CrossRefPubMed

Kim HD, Amirthalingam S, Kim SL et al (2017) Biomimetic materials and fabrication approaches for bone tissue engineering. Adv Healthc Mater 1700612:1–18. https://doi.org/10.1002/adhm.201700612CrossRef

Kommula VP, Rajulu V, Reddy KO et al (2013) International journal of polymer analysis and characterization physico-chemical, tensile, and thermal characterization of napier grass ( Native African ) fiber strands. Int J Polym Anal Ch. https://doi.org/10.1080/1023666X.2013.784935CrossRef

Kothari SC, Shivarudraiah P, Venkataramaiah SB et al (2011) Safety assessment of Cissus quadrangularis extract (CQR-300): subchronic toxicity and mutagenicity studies. Food Chem Toxicol 49:3343–3357. https://doi.org/10.1016/j.fct.2011.09.029CrossRefPubMed

Kukubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity. Biomater 27:2907–2915CrossRef

Lindman B, Karlström G, Stigsson L (2010) On the mechanism of dissolution of cellulose. J Mol Liq 156:76–81. https://doi.org/10.1016/j.molliq.2010.04.016CrossRef

Linn KF, He S, Y Song C Wang, (2016) Low temperature additive manufacturing biomimic three-dimensional hydroxyapatite / collagen scaffolds for bone regeneration. ACS Appl Mater Interfaces. https://doi.org/10.1021/acsami.6b00815CrossRef

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275CrossRef

Mate GS, Naikwade NS, Magdum CS, et al (2008) Evaluation of anti-nociceptive activity of Cissus quadrangularis on albino mice. Int. J. Green Pharm 118–121

Mayandi K, Rajini N, Pitchipoo P et al (2015a) Mechanical performance of Cissus quadrangularis / polyester composite. Mater Today Commun 4:222–232. https://doi.org/10.1016/j.mtcomm.2015.08.001CrossRef

Mayandi K, Rajini N, Pitchipoo P et al (2015b) A comparative study on characterisations of Cissus quadrangularis and Phoenix reclinata natural fibres. J Reinf Plast Compos. https://doi.org/10.1177/0731684415570045CrossRef

Mayandi K, Rajini N, Pitchipoo P, Arumugaprabhu V (2015c) Effect of alkali treatment on tensile and physicochemical characterization of Cissus quadrangularis fiber. Appl Mech Mater 814:172–178. https://doi.org/10.4028/scientific.net/AMM.813-814.172CrossRef

Morgan DML (1998) Tetrazolium (MTT) assay for cellular viability and activity. Methods Mol Biol 79:179–183PubMed

Murphy CM, O ’brien FJ, Little DG, Schindeler A (2013) Cell-scaffold interactions in the bone tissue engineering triad. Eur Cells Mater 26:120–132. https://doi.org/10.22203/eCM.v026a09

Oryan A, Alidadi S, Moshiri A, Maffulli N (2014) Bone regenerative medicine: classic options, novel strategies, and future directions. J Orthop Surg Res 9:1–27. https://doi.org/10.1186/1749-799X-9-18CrossRef

Pascual AMD, Vicente ALD (2017) Multifunctional Poly (Glycolic Acid-Co- Propylene Fumarate) electrospun fibers reinforced with grapheme oxide and hydroxyapatite nano rods. J Mater Chem B 5:4084–4096CrossRef

Qu H, Fu ZH and YS (2019) RSC advances biomaterials for bone tissue engineering scaffolds. RSC Adv. https://doi.org/10.1039/c9ra05214c

Sailaja GS, Sreenivasan K, Yokogawa Y et al (2009) Bioinspired mineralization and cell adhesion on surface functionalized poly(vinyl alcohol) films. Acta Biomater 5:1647–1655. https://doi.org/10.1016/j.actbio.2008.12.005CrossRefPubMed

Sain M, Panthapulakkal S (2006) Bioprocess preparation of wheat straw fibers and their characterization. Ind Crop Prod 23:1–8. https://doi.org/10.1016/j.indcrop.2005.01.006CrossRef

Sakkas A, Wilde F, Heufelder M et al (2017) Autogenous bone grafts in oral implantology—is it still a “gold standard”? A consecutive review of 279 patients with 456 clinical procedures. Int J Implant Dent. https://doi.org/10.1186/s40729-017-0084-4CrossRefPubMedPubMedCentral

Shin K, Acri T, Geary S, Salem AK (2017) Biomimetic mineralization of biomaterials using simulated body fluids for bone tissue engineering and regenerative medicine. Tissue Eng Part A 23:1169–1180. https://doi.org/10.1089/ten.tea.2016.0556CrossRefPubMedPubMedCentral

Shirwaikar A, Khan S, Malini S (2003) Antiosteoporotic effect of ethanol extract of Cissus quadrangularis Linn. on ovariectomized rat. J Ethnopharmacol 89:245–250. https://doi.org/10.1016/j.jep.2003.08.004CrossRefPubMed

Singh YP, Moses C (2018) Hierarchically Structured Seamless Silk Scaffolds for Osteochondral Interface Tissue Engineering. J Mater Chem B 6:5671–5689. https://doi.org/10.1039/c8tb01344fCrossRefPubMed

Singh G, Rawat P, Maurya R (2007) Constituents of Cissus quadrangularis. Nat Prod Res 21:522–528. https://doi.org/10.1080/14786410601130471CrossRefPubMed

Song J, Saiz E, Bertozzi CR (2003) A new approach to mineralization of biocompatible hydrogel scaffolds: an efficient process toward 3-dimensional bonelike composites. J Am Chem Soc 1236–1243

Stohs SJ, Ray SD (2012) A review and evaluation of the efficacy and safety of Cissus quadrangularis extracts. Phytother. Res

Sun F, Chen J, Jin S et al (2018) Development of Biomimetic Trilayer Fibrous Membranes For Guided Bone Regeneration. J Mater Chem B 7:665–675. https://doi.org/10.1039/C8tb02435aCrossRef

Tanahashi M, Yao T, Kokubo T et al (1995) Apatite coated on organic polymers by biomimetic process: improvement in its adhesion to substrate by glow-discharge treatment. J Biomed Mater Res 29:349–357CrossRef

Thein-Han WW RDKM (2009) Biomaterials Biomimetic chitosan – nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomater 5:1182–1197. https://doi.org/10.1016/j.actbio.2008.11.025CrossRef

Uff CR, Scott AD, Pockley AG, Robin KS (1995) Influence of soluble suture factors on in vitro macrophage function. Biomater 16:355–360CrossRef

Yahia S, Khalil IA, El-sherbiny IM (2019) Sandwich-like nanofibrous scaffolds for bone tissue regeneration. ACS Appl Mater Interfaces 11:28610–28620. https://doi.org/10.1021/acsami.9b06359CrossRefPubMed

Yang J, Long T, He N et al (2014) Fabrication of a chitosan/bioglass three dimensional porous scaffold for bone tissue engineering applications. J Mater Chem B Mater Biol Med 2:6611–6618. https://doi.org/10.1039/C4TB00940ACrossRefPubMed

Title: Cissus quadrangularis stem derived fiber: a natural osteoinductive substrate for regenerative bone tissue engineering
Authors: Praseetha R. Nair
S. Sreeja
G. S. Sailaja
Publication date: 10-11-2021
Publisher: Springer Netherlands
Published in: Cellulose / Issue 1/2022
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-021-04288-6

Springer Professional

Abstract

Graphical abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Springer Professional "Wirtschaft+Technik"

Other articles of this Issue 1/2022

Green carbon quantum dots from sustainable lignocellulosic biomass and its application in the detection of Fe3+

Interpreting SAXS data recorded on cellulose rich pulps

A review of interfacial bonding mechanism of bamboo fiber reinforced polymer composites

Poly ɛ-caprolactone/nanostarch composite nanofibrous wound dressing with antibacterial property and pH stimulus drug release

Methylene blue adsorption onto hydrogels made from different Eucalyptus dissolving pulps

Burst-free and sustained release of diclofenac sodium from mesoporous silica/PEI microspheres coated with carboxymethyl cellulose/chitosan layer-by-layer films