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Landscape and Ecological Engineering
Published in: Landscape and Ecological Engineering 1/2023

29-07-2022 | Special Feature: Original Paper

Comparative study on nuclear and mitochondrial DNA of Ayu Plecoglossus altivelis for environmental DNA-based spawning evaluation

Authors: Minoru Saito, Satsuki Tsuji, Ryohei Nakao, Seiji Miyazono, Yoshihisa Akamatsu

Published in: Landscape and Ecological Engineering | Issue 1/2023

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Abstract

Understandings of fish spawning are important for the effective riverine ecosystem and fisheries resources management. Application of environmental DNA (eDNA) analysis would allow fish spawning evaluation at a high temporal and spatial resolution due to its less-labor intensive property, compared to the conventional collection methods. However, the relative performance of the two eDNA-based methods used to evaluate fish spawning, pre and post comparisons of eDNA concentrations and nuclear DNA to mitochondrial DNA ratio, has not been compared in the field. We evaluated whether spawning events of Ayu Plecoglossus altivelis could be identified from day (pre-spawning) to night (post-spawning) changes in eDNA concentrations using nuclear DNA (RAG1, nuDNA) and nuDNA/mitochondrial DNA ratio in comparison to the conventional mitochondrial DNA (cyt b, mtDNA). In addition, the relative sensitivity of nuDNA concentrations in spawning evaluation was compared with mtDNA concentrations. Water samples were collected biweekly in the lower Takatsu River, western Japan, between early October and early December 2020, covering the spawning season of the fish species. Generalized linear mixed-effects models (GLMM) showed that Ayu spawning could be detected by nuDNA concentrations likewise by mtDNA concentrations, while nuDNA/mtDNA ratio could not detect spawning earlier in the spawning season. GLS models showed that the day-to-night increase in the eDNA concentrations was more profound for nuDNA than for mtDNA during the main spawning period of the fish. Our findings insist that the application of nuDNA concentrations better serves as a tool for evaluating Ayu spawning intensity in eDNA analysis.
previous article Application of environmental DNA analysis for detecting potential sources of invasive aquatic plant Egeria densa in a riverine network
next article Diurnal detection of environmental DNA of the semi-aquatic water shrew Chimarrogale platycephala using 25-h water sampling in streams

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Appendix
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Metadata
Title
Comparative study on nuclear and mitochondrial DNA of Ayu Plecoglossus altivelis for environmental DNA-based spawning evaluation
Authors
Minoru Saito
Satsuki Tsuji
Ryohei Nakao
Seiji Miyazono
Yoshihisa Akamatsu
Publication date
29-07-2022
Publisher
Springer Japan
Published in
Landscape and Ecological Engineering / Issue 1/2023
Print ISSN: 1860-1871
Electronic ISSN: 1860-188X
DOI
https://doi.org/10.1007/s11355-022-00519-5

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