Top

Published in:

01-06-2023 | Research paper

DNA:TiO₂ nanoparticle nanoassemblies: effect of temperature and nanoparticle concentration on aggregation

Authors: Evgeniya Usenko, Alexander Glamazda, Anastasiia Svidzerska, Vladimir Valeev, Anna Laguta, Sergey Petrushenko, Victor Karachevtsev

Published in: Journal of Nanoparticle Research | Issue 6/2023

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

TiO₂ nanoparticles (NPs) have unique photocatalytic properties, which are used in food industries, medicine, biosensorics, and solar energy conversion. Since the toxic properties of TiO₂ NPs have been insufficiently studied, additional information on the molecular mechanisms of their biological action on the structure and stability of biological macromolecules is needed, especially concerning DNA. In this work exploiting the differential UV–visible spectroscopy, the effect of the heating (from 20 to 90 °C) and concentration of TiO₂ NPs ((1–3) × 10⁻⁴ M) on a conformation of native DNA adsorbed on TiO₂ NPs in a buffer solution (pH 5) is studied. Analysis of dynamic light scattering (DLS) data for the DNA:TiO₂ NP suspension revealed that when the temperature increases the separated DNA:TiO₂ NP nanoassemblies form nanoaggregates. Correlation between the thermal dependency of the DLS data and thermal DNA denaturation measurements indicated that the appearance of the single-stranded unwound regions in double-stranded DNA in the suspension with temperature rise promotes the effective formation of the DNA:TiO₂ NP nanoaggregates.

Graphical abstract

previous article Composite thin film nano/microstructure of solution-based metal oxide and UV-curable polymer for liquid crystal display

next article Toluene combustion on MnOx, CeO2, and Mn-Ce-O solids prepared via citrate complexation, and citrate and urea combustion methods

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Available only for authorised users

Leroux MM, Doumandji Z, Chézeau L, Gaté L, Nahle S, Hocquel R, Zhernovkov V, Migot S, Ghanbaja J, Bonnet C, Schneider R, Rihn BH, Ferrari L, Joubert O (2020) Toxicity of TiO₂ nanoparticles: validation of alternative models. Int J Mol Sci 21(14):4855. https://doi.org/10.3390/ijms21144855CrossRef

Shabbir S, Fakhar-e-AlamKulyar M, Bhutta ZA, Boruah P, Asif M (2021) Toxicological consequences of titanium dioxide nanoparticles (TiO₂ NPs) and their jeopardy to human population. Bio Nano Sci 11:621–632. https://doi.org/10.1007/s12668-021-00836-3CrossRef

Włodarczyk R, Kwarciak-Kozłowska A (2021) Nanoparticles from the cosmetics and medical industries in legal and environmental aspects. Sustainability 13(11):5805. https://doi.org/10.3390/su13115805CrossRef

Jafari S, Mahyad B, Hashemzadeh H, Janfaza S, Gholikhani T, Tayebi L (2020) Biomedical applications of TiO₂ nanostructures: recent advances. Int J Nanomedicine 15:3447–3470. https://doi.org/10.2147/IJN.S249441CrossRef

Musial J, Krakowiak R, Mlynarczyk DT, Goslinski T, Stanisz BJ (2020) Titanium dioxide nanoparticles in food and personal care products—what do we know about their safety? Nanomaterials 10(6):1110. https://doi.org/10.3390/nano10061110CrossRef

Ziental D, Czarczynska-Goslinska B, MlynarczykDariusz T, Glowacka-Sobotta A, Stanisz B, Goslinski T, Sobotta L (2020) Titanium dioxide nanoparticles: prospects and applications in medicine. Nanomaterials 10(2):387. https://doi.org/10.3390/nano10020387CrossRef

León-Gutiérrez G, Elste JE, Cabello-Gutiérrez C, Millán-Pacheco C, Martínez-GómezMH M-A, Tiwari V, Mejía A (2022) A potent virucidal activity of functionalized TiO₂nanoparticles adsorbed with flavonoids against SARS-CoV-2. Appl Microbiol Biotechnol 106(18):5987–6002. https://doi.org/10.1007/s00253-022-12112-9CrossRef

Shah SN, Shah Z, Hussain M, Khan M (2017) Hazardous effects of titanium dioxide nanoparticles in ecosystem. Bioinorg Chem Appl 2017:4101735. https://doi.org/10.1155/2017/4101735CrossRef

Fiordaliso F, Bigini P, Salmona M, Diomede L (2022) Toxicological impact of titanium dioxide nanoparticles and food-grade titanium dioxide (E171) on human and environmental health. Environ Sci Nano 9:1199–1211. https://doi.org/10.1039/D1EN00833ACrossRef

10.

Gojznikar J, Zdravković B, Vidak M, Leskošek B, Ferk P (2022) TiO₂ nanoparticles and their effects on eukaryotic cells: a double-edged sword. Int J Mol Sci 23(20):12353. https://doi.org/10.3390/ijms232012353CrossRef

11.

Lu N, Chen Z, Song J, Weng Y, Yang G, Liu Q, Yang K, Lu X, Liu Y (2022) Size effect of TiO₂ nanoparticles as food additive and potential toxicity. Food Biophys 17:75–83. https://doi.org/10.1007/s11483-021-09695-7CrossRef

12.

Patel S, Patel P, Bakshi SR (2017) Titanium dioxide nanoparticles: an in vitro study of DNA binding, chromosome aberration assay, and comet assay. Cytotechnology 69(2):245–263. https://doi.org/10.1007/s10616-016-0054-3CrossRef

13.

Zhang X, Wang F, Liu B, Kelly EY, Servos MR, Liu J (2014) Adsorption of DNA oligonucleotides by titanium dioxide nanoparticles. Langmuir 30(3):839–845. https://doi.org/10.1021/la404633pCrossRef

14.

Li K, Du S, Van Ginkel S, Chen Y (2014) Atomic force microscopy study of the interaction of DNA and nanoparticles. In: Capco DG and Chen Y (eds) Nanomaterial, Vol. 811. Springer Dordrecht, pp 93–109. https://doi.org/10.1007/978-94-017-8739-0_6

15.

Patel S, Patel P, Undre SB, Pandya SR, Singh M, Bakshi S (2016) DNA binding and dispersion activities of titanium dioxide nanoparticles with UV/vis spectrophotometry, fluorescence spectroscopy and physicochemical analysis at physiological temperature. J Mol Liq 213:304–311. https://doi.org/10.1016/j.molliq.2015.11.002CrossRef

16.

El-Said KS, Ali EM, Kanehira K, Taniguchi A (2014) Molecular mechanism of DNA damage induced by titanium dioxide nanoparticles in toll-like receptor 3 or 4 expressing human hepatocarcinoma cell lines. J Nanobiotechnol 12:48. https://doi.org/10.1186/s12951-014-0048-2CrossRef

17.

Usenko E, Glamazda A, Valeev V, Sviderska A, Lahuta A, Petrushenko S, Karachevtsev V (2022) Effect of TiO₂ nanoparticles on the thermal stability of native DNA under UV irradiation. Appl Phys A 128:900. https://doi.org/10.1007/s00339-022-06043-5CrossRef

18.

Bae S, Oh I, Yoo J, Kim JS (2021) Effect of DNA flexibility on complex formation of a cationic nanoparticle with double-stranded DNA. ACS Omega 6(29):18728–18736. https://doi.org/10.1021/acsomega.1c01709CrossRef

19.

Cleaves HJ, Jonsson CM, Jonsson CL, Sverjensky DA, Hazen RM (2010) Adsorption of nucleic acid components on rutile (TiO₂) surfaces. Astrobiology 10(3):311–323. https://doi.org/10.1089/ast.2009.0397CrossRef

20.

Ma L, Liu B, Huang P-JJ, Zhang X, Liu J (2016) DNA Adsorption by ZnO nanoparticles near its solubility limit: implications for DNA fluorescence quenching and DNAzyme activity assays. Langmuir 32(22):5672–5680. https://doi.org/10.1021/acs.langmuir.6b00906CrossRef

21.

He Q, Wu Q, Feng X, Liao Z, Peng W, Liu Y, Peng D, Liu Z, Mo M (2020) Interfacing DNA with nanoparticles: surface science and its applications in biosensing. Int J Biol Macromol 151:757–780. https://doi.org/10.1016/j.ijbiomac.2020.02.217CrossRef

22.

Das S, Chatterjee S, Pramanik S, Devi PS, Kumar GS (2018) A new insight into the interaction of ZnO with calf thymus DNA through surface defects. J Photochem Photobiol B 178:339–347. https://doi.org/10.1016/j.jphotobiol.2017.10.039CrossRef

23.

Peng B, Liu Z, Jiang Y (2022) Aggregation of DNA-grafted nanoparticles in water: the critical role of sequence-dependent conformation of DNA coating. J Phys Chem B 126(4):847–857. https://doi.org/10.1021/acs.jpcb.1c09450CrossRef

24.

An H, Liu Q, Ji Q, Jin B (2010) DNA binding and aggregation by carbon nanoparticles. Biochem Biophys Res Commun 393(4):571–576. https://doi.org/10.1016/j.bbrc.2010.02.006CrossRef

25.

Sorokin VA, Valeev VA, Usenko EL, Andrushchenko VV (2012) DNA conformational equilibrium in the presence of Zn²⁺ ions in neutral and alkaline solutions. Int J Biol Macromol 50(3):854–860. https://doi.org/10.1016/j.ijbiomac.2011.11.011

26.

Svanberg C, Bergman R (2020) Photon correlation spectroscopy. Definitions. https://doi.org/10.32388/hfvlwa

27.

Tscharnuter W (2006) Photon correlation spectroscopy in particle sizing. In: Meyers RA and Flippen RB (eds) Encyclopedia of Analytical Chemistry. John Wiley & Sons Ltd. https://doi.org/10.1002/9780470027318.a1512

28.

Nobbmann U, Morfesis A (2008) Characterization of nanoparticles by light scattering. MRS Online Proc Libr 1074:10741045. https://doi.org/10.1557/PROC-1074-I10-45CrossRef

29.

Cascio C, Geiss O, Franchini F, Ojea-Jimenez I, Rossi F, Gilliland D, Calzolai L (2015) Detection, quantification and derivation of number size distribution of silver nanoparticles in antimicrobial consumer products. J Anal At Spectrom 30(6):1255–1265. https://doi.org/10.1039/C4JA00410HCrossRef

30.

de la Calle I, Menta M, Klein M, Maxit B, Séby F (2018) Towards routine analysis of TiO₂ (nano-)particle size in consumer products: evaluation of potential techniques. Spectrochim Acta Part B 147:28–42. https://doi.org/10.1016/j.sab.2018.05.012CrossRef

31.

Krause B, Meyer T, Sieg H, Kästner C, Reichardt P, Tentschert J, Jungnickel H, Estrela-Lopis I, Burel A, Chevance S, Gauffre F, Jalili P, Meijer J, Böhmert L, Braeuning A, Thünemann AF, Emmerling F, Fessard V, Laux P, Lampen A, Luch A (2018) Characterization of aluminum, aluminum oxide and titanium dioxide nanomaterials using a combination of methods for particle surface and size analysis. RSC Adv 8(26):14377–14388. https://doi.org/10.1039/C8RA00205C

32.

Souza TGF, Ciminelli VST, Mohallem NDS (2016) A comparison of TEM and DLS methods to characterize size distribution of ceramic nanoparticles. J Phys Conf Ser 733:012039. https://doi.org/10.1088/1742-6596/733/1/012039CrossRef

33.

Zetasizer nano user manual, Malvern Instruments Ltd. (2013) Worcestershire WR14 1XZ, UK

34.

Romanello MB, Fidalgo de Cortalezzi MM (2013) An experimental study on the aggregation of TiO₂ nanoparticles under environmentally relevant condition. Water Res 47(12):3887–3898. https://doi.org/10.1016/j.watres.2012.11.061CrossRef

35.

Higasi K, Baba H, Rembaum A (1965) Quantum organic chemistry. John Wiley & Sons Ltd, New York

36.

Usenko EL, Valeev VA, Glamazda AY, Karachevtsev VA (2020) The effect of divalent metal ions on the temperature stability of poly(I:C) duplex. J Spectrosc 2020:8850214. https://doi.org/10.1155/2020/8850214CrossRef

37.

Sorokin VA, Valeev VA, Usenko EL, Andrushchenko VV (2011) Divalent metal ion effect on helix–coil transition of high molecular weight DNA in neutral and alkaline solutions. Int J Biol Macromol 48(2):369–374. https://doi.org/10.1016/j.ijbiomac.2010.12.016CrossRef

38.

Sharma VK (2009) Aggregation and toxicity of titanium dioxide nanoparticles in aquatic environment. J Environ Sci Health A Tox Hazard Subst Environ Eng 44(14):1485–1495. https://doi.org/10.1080/10934520903263231CrossRef

39.

Pellegrino F, Pellutiè L, Sordello F, Minero C, Ortel E, Hodoroaba V-D, Maurino V (2017) Influence of agglomeration and aggregation on the photocatalytic activity of TiO₂ nanoparticles. Appl Catal B 216:80–87. https://doi.org/10.1016/j.apcatb.2017.05.046CrossRef

40.

Blagoi Y, Gladchenko G, Nafie LA, Freedman TB, Sorokin V, Valeev V, He Y (2005) Phase equilibrium in poly(rA)·poly(rU) complexes with Cd²⁺ and Mg²⁺ ions, studied by ultraviolet, infrared, and vibrational circular dichroism spectroscopy. Biopolymers 78(5):275–286. https://doi.org/10.1002/bip.20288CrossRef

41.

Kutsevol N, Glamazda A, Chumachenko V, Harahuts Yu, Stepanian SG, Plokhotnichenko AM, Karachevtsev VA (2018) Behavior of hybrid thermosensitive nanosystem dextran-graft-PNIPAM/gold nanoparticles: characterization within LCTS. J Nanopart Res 20:236. https://doi.org/10.1007/s11051-018-4331-2CrossRef

Title: DNA:TiO2 nanoparticle nanoassemblies: effect of temperature and nanoparticle concentration on aggregation
Authors: Evgeniya Usenko
Alexander Glamazda
Anastasiia Svidzerska
Vladimir Valeev
Anna Laguta
Sergey Petrushenko
Victor Karachevtsev
Publication date: 01-06-2023
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 6/2023
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-023-05770-x

Springer Professional

Abstract

Graphical abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Other articles of this Issue 6/2023

Photoconductive control of high-order localized surface plasmon modes in Au-Si-Au nanodisk stacking

Mesoscopic simulations of protein corona formation on zwitterionic peptide-grafted gold nanoparticles

A model for the removal of Cr(VI) in water by nanoscale zero-valent iron: quantifying the reaction process and calculating the electron efficiency

Dopamine-modified nanospheres of oxalic-bimetal for T1/T2 dual-modal MRI and photothermal tumour treatment

In situ electron microscopy techniques for nanoparticle dispersion analysis of commercial sunscreen

Synthesis and characterization of MSN/Fe3O4/Gd2O3 nanocomposite as theranostic systems

Premium Partners