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Journal of Materials Engineering and Performance

Published in:

01-07-2011

Expression of TGF-β in Fractures Fixed by Nitinol Swan-like Memory Compressive Connectors

Authors: M. Li, C. C. Zhang, S. G. Xu, Q. G. Fu

Published in: Journal of Materials Engineering and Performance | Issue 4-5/2011

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Abstract

In this article, the effect of internal fixation of a Nitinol swan-like memory compressive connector (SMC) on the temporal expression of transforming growth factor-β (TGF-β) at fracture sites is evaluated. Specimens were collected from 35 New Zealand rabbits modeled for bilateral humeral fracture fixed with either SMC or stainless dynamic compression plate (DCP). Five rabbits each were killed at day 1, 3, 7, 14, 21, 28, and 56. The local positive staining potency, positive area ratio, and positive index of TGF-β were measured using an immunohistochemistry approach (EnVision) in combination with a computerized image analysis system. TGF-β staining was seen in mesenchymal cells, osteoblasts, chondrocytes, and in the extracellular matrix of fractures fixed in both the SMC and the DCP samples without a significant difference in staining at both the early stages (days 1 and 3) and day 56. A higher TGF-β content was observed in the fractures fixed with SMC when compared to that of DCP from day 7 to 28. As a conclusion, TGF-β is highly expressed in fractures fixed with SMC during chondrogenesis stage and entochondrostosis stage. Finally, the mechanism of how SMC promoting synthesis and secretion of TGF-β in the process of fracture healing has been discussed.

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C.C. Zhang, S.G. Xu, J.L. Wang, B.Q. Yu, Q.G. Wang, Q.L. Zhang, Y.T. Liang, and R. Wang, Design and Clinical Application of Swan-like Memory Compressive Connector for Upper Limb Diaphysis, Acad. J. Sec. Mil. Med. Univ., 2001, 22, p 939–942 (in Chinese)

Q.G. Fu, X.W. Liu, S.G. Xu, M. Li, and C.C. Zhang, Stress-Shielding Effect of Nitinol Swan-Like Memory Compressive Connector on Fracture Healing of Upper Limb, J. Mater. Eng. Perform., 2009, 18, p 797–804CrossRef

S.G. Xu, C.C. Zhang, J.C. Su, X.H. Liu, Y.N. Wu, J.G. Wu, Z.J. Xue, and Z.Q. Ding, Three-Dimensional Element Analysis of Swan-like Memory Compressive Connector for Treating Fractures and Nonunion of Humerus, Acad. J. Sec. Mil. Med. Univ., 2001, 22, p 943–945 (in Chinese)

M.E. Bolander, Regulation of Fracture Repair by Growth Factors, Proc. Soc. Exp. Bio. Med., 1992, 200, p 165–170CrossRef

D.M. Rosen, S.A. Stempien, A.Y. Thompson, and S.M. Seyedin, Transforming Growth Factor-beta Modulates the Expression of Osteoblast and Chondroblast Phenotypes In Vitro, J. Cell. Physiol., 1988, 134, p 337–346CrossRef

L.S. Beck, R.L. Wong, L. DeGuzman, W.P. Lee, B. Ongpipattanakul, and T.H. Nguyen, Combination of Bone Marrow and TGF-beta1 Augment the Healing of Critical-sized Bone Defects, J. Pharm. Sci., 1998, 87, p 1379–1386CrossRef

B.A. Vosse, W. Seelentag, and A. Bachmann, Background Staining of Visualization Systems in Immunohistochemistry: Comparison of the Avidin-Biotin Complex System and the EnVision + System, Appl. Immunohistochem. Mol. Morphol., 2007, 15, p 103–107CrossRef

U. Kammerer, M. Kapp, and A.M. Gassel, A New Rapid Immunohistochemical Staining Technique Using the EnVision Antibody Complex, J. Histochem. Cytochem., 2001, 49, p 623–630CrossRef

O.O. Oni, The Early Stages of the Repair of Adult Human Diaphyseal Fractures, Injury, 1997, 28, p 521–525CrossRef

10.

A.D. Bakker, J. Klein-Nulend, E. Tanck, I.C. Heyligers, G.H. Albers, P. Lips, and E.H. Burger, Different Responsiveness to Mechanical Stress of Bone Cells from Osteoporotic Versus Osteoarthritic Donors, Int. Osteoporos., 2006, 17, p 827–833CrossRef

11.

A.J. van Wamel, C. Ruwhof, L.J. van der Valk-Kokshoorn, P.I. Schrier, and A. van der Laarse, Stretch-induced Paracrine Hypertrophic Stimuli Increase TGF-beta1 Expression in Cardiomyocytes, Mol. Cell. Biochem., 2002, 236, p 147–153CrossRef

12.

J. Keski-Oja, K. Koli, and H. von Melchner, TGF-beta Activation by Traction?, Trends Cell Biol., 2004, 14, p 657–659CrossRef

13.

W.J. Yan, C.C. Zhang, X.S. Zhang, J.L. Wang, H.X. Shen, S.G. Xu, and J.C. Su, Effects of Swan-like Memory Compressive Connector on Healing of Femoral Fractures in Dogs, Acad. J. Sec. Mil. Med. Univ., 2001, 22, p 952–954 (in Chinese)

Title: Expression of TGF-β in Fractures Fixed by Nitinol Swan-like Memory Compressive Connectors
Authors: M. Li
C. C. Zhang
S. G. Xu
Q. G. Fu
Publication date: 01-07-2011
Publisher: Springer US
Published in: Journal of Materials Engineering and Performance / Issue 4-5/2011
Print ISSN: 1059-9495
Electronic ISSN: 1544-1024
DOI: https://doi.org/10.1007/s11665-011-9914-3

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