Skip to main content
Disciplines
Automotive Business IT + Informatics Construction + Real Estate Electrical Engineering + Electronics Energy + Sustainability Insurance + Risk Finance + Banking Management + Leadership Marketing + Sales Mechanical Engineering + Materials
Events
DE
EN
Books
Journals
Topic Page
Marketing
Start single access now
Access for companies
EXTENDED SEARCH
Log in
Top
Cellulose
Published in: Cellulose 12/2018

15-10-2018 | Original Paper

Hybrid scaffolds enhanced by nanofibers improve in vitro cell behavior for tissue regeneration

Authors: Baoxiu Wang, Chengsheng Huang, Shiyan Chen, Xueyu Xing, Minghao Zhang, Qingkai Wu, Huaping Wang

Published in: Cellulose | Issue 12/2018

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

To construct a biomimetic scaffold with a nanoscale structure similar to that of natural tissue, the objective of this work was to prepare three-dimensional (3D) porous hybrid scaffolds based on gelatin and bacterial cellulose nanofibers for tissue regeneration. The nanofibrous structure, water absorption, and compressive mechanical properties of the scaffolds were studied. The hybrid scaffolds not only give a sufficiently porous structure for efficient nutrient transport and vascularization, but also provide the nanofibrous structure and improve the roughness of the scaffold pore walls. The hybrid scaffolds also exhibit higher modulus as stiffness compared to the pure gelatin scaffold. The viability and morphology of Pig iliac endothelial cells (PIECs) cultured on the 3D scaffolds were examined. PIECs adhered and proliferated better on the stiff hybrid scaffold with nanofibers compared to the soft gelatin scaffold without nanofibers. The results addressed the effect of the nanofibers and the stiffness of scaffolds on cell behavior, and the biomimetic nanofibrous hybrid scaffolds would be highly favorable/desired for tissue regeneration, e.g., skin and urethral regeneration.

Graphical Abstract

previous article Cellulose nanofibrils as a replacement for xanthan gum (XGD) in water based muds (WBMs) to be used in shale formations
next article Hybrid nanopaper of cellulose nanofibrils and PET microfibers with high tear and crumpling resistance

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now
Appendix
Available only for authorised users
Literature
Arima Y, Iwata H (2007) Effect of wettability and surface functional groups on protein adsorption and cell adhesion using well-defined mixed self-assembled monolayers. Biomaterials 28:3074–3082CrossRef
Atala A (2004) Tissue engineering for the replacement of organ function in the genitourinary system. Am J Transpl 4:58–73CrossRef
Ataollahi F, Pramanik S, Moradi A, Dalilottojari A, Pingguan-Murphy B, Abas WABW, Abu Osman NA (2015) Endothelial cell responses in terms of adhesion, proliferation, and morphology to stiffness of polydimethylsiloxane elastomer substrates. J Biomed Mater Res Part A 103:2203–2213CrossRef
Bodin A, Ahrenstedt L, Fink H, Brumer H, Risberg B, Gatenholm P (2007) Modification of nanocellulose with a xyloglucan-RGD conjugate enhances adhesion and proliferation of endothelial cells: implications for tissue engineering. Biomacromolecules 8:3697–3704CrossRef
Bodin A, Bharadwaj S, Wu S, Gatenholm P, Atala A, Zhang Y (2010) Tissue-engineered conduit using urine-derived stem cells seeded bacterial cellulose polymer in urinary reconstruction and diversion. Biomaterials 31:8889–8901CrossRef
Chan B, Leong K (2008) Scaffolding in tissue engineering: general approaches and tissue-specific considerations. Eur Spine J 17:467–479CrossRef
Chen F, Yoo JJ, Atala A (1999) Acellular collagen matrix as a possible “off the shelf” biomaterial for urethral repair. Urology 54:407–410CrossRef
Ding S-J, Shie M-Y, Wei C-K (2011) In vitro physicochemical properties, osteogenic activity, and immunocompatibility of calcium silicate-gelatin bone grafts for load-bearing applications. ACS Appl Mater Interfaces 3:4142–4153CrossRef
Fagerholm P, Lagali NS, Ong JA, Merrett K, Jackson WB, Polarek JW, Suuronen EJ, Liu Y, Brunette I, Griffith M (2014) Stable corneal regeneration four years after implantation of a cell-free recombinant human collagen scaffold. Biomaterials 35:2420–2427CrossRef
Hirai A, Inui O, Horii F, Tsuji M (2009) Phase separation behavior in aqueous suspensions of bacterial cellulose nanocrystals prepared by sulfuric acid treatment. Langmuir 25:497–502CrossRef
Huang Y, Zhu C, Yang J, Nie Y, Chen C, Sun D (2014) Recent advances in bacterial cellulose. Cellulose 21:1–30CrossRef
Jia W, Tang H, Wu J, Hou X, Chen B, Chen W, Zhao Y, Shi C, Zhou F, Yu W (2015) Urethral tissue regeneration using collagen scaffold modified with collagen binding VEGF in a beagle model. Biomaterials 69:45–55CrossRef
Jin G, He R, Sha B, Li W, Qing H, Teng R, Xu F (2018) Electrospun three-dimensional aligned nanofibrous scaffolds for tissue engineering. Mater Sci Eng C 92:995–1005CrossRef
Jithendra P, Rajam AM, Kalaivani T, Mandal AB, Rose C (2013) Preparation and characterization of aloe vera blended collagen–chitosan composite scaffold for tissue engineering applications. ACS Appl Mater Interfaces 5:7291–7298CrossRef
Khan S, Ul-Islam M, Ikram M, Ullah MW, Israr M, Subhan F, Kim Y, Jang JH, Yoon S, Park JK (2016) Three-dimensionally microporous and highly biocompatible bacterial cellulose-gelatin composite scaffolds for tissue engineering applications. RSC Adv 6:110840–110849CrossRef
Kharaziha M, Nikkhah M, Shin S-R, Annabi N, Masoumi N, Gaharwar AK, Camci-Unal G, Khademhosseini A (2013) PGS: gelatin nanofibrous scaffolds with tunable mechanical and structural properties for engineering cardiac tissues. Biomaterials 34:6355–6366CrossRef
Kilian KA, Bugarija B, Lahn BT, Mrksich M (2010) Geometric cues for directing the differentiation of mesenchymal stem cells. Proc Natl Acad Sci 107:4872–4877CrossRef
Kokubo T, Kim H-M, Kawashita M (2003) Novel bioactive materials with different mechanical properties. Biomaterials 24:2161–2175CrossRef
Kucinska-Lipka J, Gubanska I, Janik H (2015) Bacterial cellulose in the field of wound healing and regenerative medicine of skin: recent trends and future prospectives. Polym Bull 72:2399–2419CrossRef
Levengood SKL, Zhang M (2014) Chitosan-based scaffolds for bone tissue engineering. J Mater Chem B 2:3161–3184CrossRef
Li Z, Wang L, Chen S, Feng C, Chen S, Yin N, Yang J, Wang H, Xu Y (2015) Facilely green synthesis of silver nanoparticles into bacterial cellulose. Cellulose 22:373–383CrossRef
Liu X, Smith LA, Hu J, Ma PX (2009) Biomimetic nanofibrous gelatin/apatite composite scaffolds for bone tissue engineering. Biomaterials 30:2252–2258CrossRef
Loh QL, Choong C (2013) Three-dimensional scaffolds for tissue engineering applications: role of porosity and pore size. Tissue Eng Part B Rev 19:485–502CrossRef
Min B-M, Lee G, Kim SH, Nam YS, Lee TS, Park WH (2004) Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials 25:1289–1297CrossRef
Mohandas A, Anisha B, Chennazhi K, Jayakumar R (2015) Chitosan–hyaluronic acid/VEGF loaded fibrin nanoparticles composite sponges for enhancing angiogenesis in wounds. Colloids Surf B Biointerfaces 127:105–113CrossRef
Nguyen LT, Chen S, Elumalai NK, Prabhakaran MP, Zong Y, Vijila C, Allakhverdiev SI, Ramakrishna S (2013) Biological, chemical, and electronic applications of nanofibers. Macromol Mater Eng 298:822–867CrossRef
Ou K, Wu X, Wang B, Meng C, Dong X, He J (2017) Controlled in situ graft polymerization of DMAEMA onto cotton surface via SI-ARGET ATRP for low-adherent wound dressings. Cellulose 24:5211–5224CrossRef
Park S, Park J, Jo I, Cho S-P, Sung D, Ryu S, Park M, Min K-A, Kim J, Hong S, Hong BH, Kim B-S (2015) In situ hybridization of carbon nanotubes with bacterial cellulose for three-dimensional hybrid bioscaffolds. Biomaterials 58:93–102CrossRef
Petersen N, Gatenholm P (2011) Bacterial cellulose-based materials and medical devices: current state and perspectives. Appl Microbiol Biotechnol 91:1277CrossRef
Powell HM, Boyce ST (2006) EDC cross-linking improves skin substitute strength and stability. Biomaterials 27:5821–5827CrossRef
Pramanik S, Pingguan-Murphy B, Abu Osman NA (2012) Progress of key strategies in development of electrospun scaffolds: bone tissue. Sci Technol Adv Mater 13:043002–043014CrossRef
Schindler M, Ahmed I, Kamal J, Nur-E-Kamal A, Grafe TH, Chung HY, Meiners S (2005) A synthetic nanofibrillar matrix promotes in vivo-like organization and morphogenesis for cells in culture. Biomaterials 26:5624–5631CrossRef
Selim M, Bullock AJ, Blackwood KA, Chapple CR, MacNeil S (2011) Developing biodegradable scaffolds for tissue engineering of the urethra. BJU Int 107:296–302CrossRef
Song LJ, Xu YM, Hu XY, Zhang HZ (2008) Urethral substitution using autologous lingual mucosal grafts: an experimental study. BJU Int 101:739–743CrossRef
Stevens MM, George JH (2005) Exploring and engineering the cell surface interface. Science 310:1135–1138CrossRef
Sunyer R, Jin AJ, Nossal R, Sackett DL (2012) Fabrication of hydrogels with steep stiffness gradients for studying cell mechanical response. PLoS ONE 7:46107–46115CrossRef
Svensson A, Nicklasson E, Harrah T, Panilaitis B, Kaplan D, Brittberg M, Gatenholm P (2005) Bacterial cellulose as a potential scaffold for tissue engineering of cartilage. Biomaterials 26:419–431CrossRef
Vatankhah E, Prabhakaran MP, Jin G, Mobarakeh LG, Ramakrishna S (2014) Development of nanofibrous cellulose acetate/gelatin skin substitutes for variety wound treatment applications. J Biomater Appl 28:909–921CrossRef
Wang X, Ding B, Li B (2013) Biomimetic electrospun nanofibrous structures for tissue engineering. Mater Today 16:229–241CrossRef
Wells RG (2008) The role of matrix stiffness in regulating cell behavior. Hepatology 47:1394–1400CrossRef
Woo KM, Chen VJ, Ma PX (2003) Nano-fibrous scaffolding architecture selectively enhances protein adsorption contributing to cell attachment. J Biomed Mater Res A 67:531–537CrossRef
Xie J, Peng C, Zhao Q, Wang X, Yuan H, Yang L, Li K, Lou X, Zhang Y (2016) Osteogenic differentiation and bone regeneration of iPSC-MSCs supported by a biomimetic nanofibrous scaffold. Acta Biomater 29:365–379CrossRef
Xu T, Miszuk JM, Zhao Y, Sun H, Fong H (2015) Electrospun polycaprolactone 3D nanofibrous scaffold with interconnected and hierarchically structured pores for bone tissue engineering. Adv Healthc Mater 4:2238–2246CrossRef
Yang F, Murugan R, Ramakrishna S, Wang X, Ma Y-X, Wang S (2004) Fabrication of nano-structured porous PLLA scaffold intended for nerve tissue engineering. Biomaterials 25:1891–1900CrossRef
Yang F, Murugan R, Wang S, Ramakrishna S (2005) Electrospinning of nano/micro scale poly(l-lactic acid) aligned fibers and their potential in neural tissue engineering. Biomaterials 26:2603–2610CrossRef
Yao J, Chen S, Chen Y, Wang B, Pei Q, Wang H (2017) Macrofibers with high mechanical performance based on aligned bacterial cellulose nanofibers. ACS Appl Mater Interfaces 9:20330–20339CrossRef
Yeh Y-T, Hur SS, Chang J, Wang K-C, Chiu J-J, Li Y-S, Chien S (2012) Matrix stiffness regulates endothelial cell proliferation through septin 9. PLoS ONE 7:46889–46894CrossRef
Yin N, Stilwell MD, Santos TMA, Wang H, Weibel DB (2015) Agarose particle-templated porous bacterial cellulose and its application in cartilage growth in vitro. Acta Biomater 12:129–138CrossRef
Zhang P, Wu H, Wu H, Lù Z, Deng C, Hong Z, Jing X, Chen X (2011) RGD-conjugated copolymer incorporated into composite of poly (lactide-co-glycotide) and poly (l-lactide)-grafted nanohydroxyapatite for bone tissue engineering. Biomacromolecules 12:2667–2680CrossRef
Zheng X, Zhang Q, Liu J, Pei Y, Tang K (2016) A unique high mechanical strength dialdehyde microfibrillated cellulose/gelatin composite hydrogel with a giant network structure. RSC Adv 6:71999–72007CrossRef
Metadata
Title
Hybrid scaffolds enhanced by nanofibers improve in vitro cell behavior for tissue regeneration
Authors
Baoxiu Wang
Chengsheng Huang
Shiyan Chen
Xueyu Xing
Minghao Zhang
Qingkai Wu
Huaping Wang
Publication date
15-10-2018
Publisher
Springer Netherlands
Published in
Cellulose / Issue 12/2018
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI
https://doi.org/10.1007/s10570-018-2087-6

Other articles of this Issue 12/2018

Cellulose 12/2018 Go to the issue

Original Paper

Quantification of accessible hydroxyl groups in cellulosic pulps by dynamic vapor sorption with deuterium exchange

Original Paper

Chemically cross-linked aerogels based on cellulose nanocrystals and polysilsesquioxane

Original Paper

Super-compressible, fatigue resistant and anisotropic carbon aerogels for piezoresistive sensors

Original Paper

A new approach to improve dissolving pulp properties: spraying cellulase on rewetted pulp at a high fiber consistency

Original Paper

Bacterial cellulose derived monolithic titania aerogel consisting of 3D reticulate titania nanofibers

Original Paper

Hybrid nanopaper of cellulose nanofibrils and PET microfibers with high tear and crumpling resistance