Top

Journal of Sol-Gel Science and Technology

Published in:

25-05-2023 | Original Paper: Sol-gel and hybrid materials for biological and health (medical) applications

Hydroxyapatite−loaded starch/polyvinyl alcohol scaffold for bone regeneration application: preparation and characterization

Authors: Thi Duy Hanh Le, Huynh Nguyen Anh Tuan, Van Tien Nguyen, Anh Thi Le

Published in: Journal of Sol-Gel Science and Technology | Issue 2/2023

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Hydroxyapatite (HA) embedded in polymer-based bone scaffold exhibits excellent medical properties for bone healing. Indeed, limited studies have reported on the addition of HA into starch/polyvinyl alcohol (PVA) scaffold system, especially HA role as bio-additive releasing Ca²⁺ ion promoting biomineralization. Herein, HA derived from chicken bone with the particles size ranging from 100 to 600 nm loaded starch/PVA composite scaffold with different amount was fabricated using a salt-leaching method. XRD and SEM-EDS analysis indicated the presence of HA in starch/PVA composite. FTIR results showed that the chemical bondings in starch/PVA matrices were not affected by the introduction of HA. Morphology and architecture of scaffolds characterized by SEM demonstrated pore size and their structure satisfying the needs of bone scaffolds. Young modulus of composite scaffold increased with the increase of HA loading content. The Ca²⁺ release analyzed by ion chromatography system showed the increasing trend with amount of HA addition into scaffold, which improved mineralization of composite scaffold in in-vitro observed by SEM-EDS. HA-loaded starch/PVA scaffold demonstrated a similar biodegradation rate and amount to the starch/ PVA scaffold. According to the result, loading starch/PVA with HA can be proposed a potential candidate for bone regeneration.

Graphical Abstract

previous article Sol–gel derived Zn doped TiO2 thin films and their waveguides

next article Sol–gel derived iron-manganese oxide nanoparticles: a promising dual-functional material for solar photocatalysis and antimicrobial applications

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Amini AR, Laurencin CT, Nukavarapu SP (2012) Bone tissue engineering: recent advances and challenges. Crit Rev Biomed Eng 40(5):363–408CrossRef

Roseti L, Parisi V, Petretta M, Cavallo C, Desando G, Bartolotti I, Grigolo B (2017) Scaffolds for bone tissue engineering: State of the art and new perspectives. Mater Sci Eng C 78:1246–1262CrossRef

Le TDH, Liaudanskaya V, Bonani W, Migliares C, Motta A (2020) Diatom Particles: A Promising Osteoinductive Agent of Silk Fibroin-Based Scaffold for Bone Regeneration. IFMBE Proceedings 69. Springer, Singapore, p 147–151

Pati F, Song TH, Rijal G, Jang J, Kim WS, Cho DW (2015) Ornamenting 3D printed scaffolds with cell-laid extracellular matrix for bone tissue regeneration. Biomaterials 37:230–241CrossRef

Blaker JJ, Maquet V, Jérôme R, Boccaccini AR, Nazhat SN (2005) Mechanical properties of highly porous PDLLA/Bioglass composite foams as scaffolds for bone tissue engineering. Acta Biomater 1(6):643–652CrossRef

Olszta MJ, Cheng X, Jee SS, Kumar R, Kim YY, Kaufman MJ, Douglas EP, Gower LB (2007) Bone structure and formation: A new perspective. Mater Sci Eng R Rep. 58(3–5):77–116CrossRef

Thorwarth M, Schultze-Mosgau S, Kessler P, Wiltfang J, Schlegel KA (2005) Bone regeneration in osseous defects using a resorbable nanoparticular hydroxyapatite. J Oral Maxillofac Surg 63(11):1626–1633CrossRef

Fang J, Li P, Lu X, Fang L, Lü X, Ren F (2019) A strong, tough, and osteoconductive hydroxyapatite mineralized polyacrylamide/dextran hydrogel for bone tissue regeneration. Acta Biomater 88:503–513CrossRef

Lew D, Farrell B, Bardach J, Keller J (1997) Repair of craniofacial defects with hydroxyapatite cement. J Oral Maxillofac Surg 55(12):1441–1449CrossRef

10.

Ratnayake JTB, Mucalo M, Dias GJ (2017) Substituted hydroxyapatites for bone regeneration: A review of current trends. J Biomed Mater Res - Part B Appl Biomater 105(5):1285–1299CrossRef

11.

Darimont GL, Cloots R, Heinen E, Seidel L, Legrand R (2002) In vivo behaviour of hydroxyapatite coatings on titanium implants: A quantitative study in the rabbit. Biomaterials 23(12):2569–2575CrossRef

12.

Kattimani VS, Kondaka S, Lingamaneni KP (2016) Hydroxyapatite–Past, Present, and Future in Bone Regeneration. Bone Tissue Regen Insights 7:9–18

13.

Caria PHF, Kawachi EY, Bertran CA, Camilli JA (2007) Biological assessment of porous-implant hydroxyapatite combined with periosteal grafting in maxillary defects. J Oral Maxillofac Surg 65(5):847–854CrossRef

14.

Huang X, Bai S, Lu Q, Liu X, Liu S, Zhu H (2015) Osteoinductive-nanoscaled silk/HA composite scaffolds for bone tissue engineering application. J Biomed Mater Res - Part B Appl Biomater 103(7):1402–1414CrossRef

15.

Hallman M, Driscoll JA, Lubbe R, Jeon S, Chang K, Haleem M, Jakus A, Pahapill R, Yun C, Shah R, Hsu WK, Stock SR, Hsu EL (2021) Influence of geometry and architecture on the in vivo success of 3D-printed scaffolds for spinal. Fusion Tissue Eng - Part A 27(1–2):26–36CrossRef

16.

Jin P, Liu L, Cheng L, Chen X, Xi S, Jiang T (2023) Calcium-to-phosphorus releasing ratio affects osteoinductivity and osteoconductivity of calcium phosphate bioceramics in bone tissue engineering. Biomed Eng Online 22(1):1–16CrossRef

17.

Meagher MJ, Weiss-Bilka HE, Best ME, Boerckel JD, Wagner DR, Roeder RK (2016) Acellular hydroxyapatite-collagen scaffolds support angiogenesis and osteogenic gene expression in an ectopic murine model: Effects of hydroxyapatite volume fraction. J Biomed Mater Res—Part A 104(9):2178–2188CrossRef

18.

Ielo I, Calabrese G, De Luca G, Conoci S (2022) Recent advances in hydroxyapatite-based biocomposites for bone tissue regeneration in orthopedics. Int J Mol Sci 23:17CrossRef

19.

Hasegawa S, Tamura J, Neo M, Shikinami Y, Saito M, Kita M, Nakamura T (2005) In vivo evaluation of a porous hydroxyapatite/poly-DL-lactide composite for use as a bone substitute. J Biomed Mater Res—Part A 75(3):567–579CrossRef

20.

Martin V, Ribeiro IA, Alves MM, Gonçalves L, Claudio RA, Grenho L, Fernandes MH, Gomes P, Santos CF, Bettencourt AF (2019) Engineering a multifunctional 3D-printed PLA-collagen-minocycline-nanoHydroxyapatite scaffold with combined antimicrobial and osteogenic effects for bone regeneration. Mater Sci Eng C 101:15–26CrossRef

21.

Wang Y, Guo Y, Wei Q, Li X, Ji K, Zhang K (2021) Current researches on design and manufacture of biopolymer-based osteochondral biomimetic scaffolds. Bio-Des Manuf 4(3):541–567CrossRef

22.

Gao X, Xu Z, Li S, Cheng L, Xu D, Li L, Chen L, Xu Y, Liu Z, Liu Y, Sun J (2023) Chitosan-vancomycin hydrogel incorporated bone repair scaffold based on staggered orthogonal structure: a viable dually controlled drug delivery system. RSC Adv 13(6):3759–3765CrossRef

23.

Saboktakin MR, Akhyari S, Nasirov FA (2014) Synthesis and characterization of modified starch/polybutadiene as novel transdermal drug delivery system. Int J Biol Macromol 69:442–446CrossRef

24.

Marto J, Ribeiro H M, Almeida A J(2020) Starch-based nanocapsules as drug carriers for topical drug delivery Smart Nanocontainers: Micro Nano Technol 1:287–294CrossRef

25.

Marto JM, Gouveia LF, Gonçalves LMD, Ribeiro HM, Almeida AJ (2018) Design of minocycline-containing starch nanocapsules for topical delivery. J Microencapsul 35(4):344–356CrossRef

26.

Santander-Ortega MJ, Stauner T, Loretz B, Ortega-Vinuesa JL, Bastos-González D, Wenz G, Schaefer UF, Lehr CM (2010) Nanoparticles made from novel starch derivatives for transdermal drug delivery. J Control Release 141(1):85–92CrossRef

27.

Mohammad DM, Stiharu I (2022) Preparing and characterizing novel biodegradable starch/PVA-based films with nano-sized zinc-oxide particles for wound-dressing applications. Appl Sci 12:4001CrossRef

28.

Rodrigues AI, Gomes ME, Leonor IB, Reis RL (2012) Bioactive starch-based scaffolds and human adipose stem cells are a good combination for bone tissue engineering. Acta Biomater 8(10):3765–3776CrossRef

29.

Shahriarpanah S, Nourmohammadi J, Amoabediny G (2016) Fabrication and characterization of carboxylated starch-chitosan bioactive scaffold for bone regeneration. Int J Biol Macromol 93:1069–1078CrossRef

30.

Manavitehrani I, Fathi A, Wang Y, Maitz PK, Mirmohseni F, Cheng TL, Peacock L, Little DG, Schindeler A, Dehghani F (2017) Fabrication of a biodegradable implant with tunable characteristics for bone implant applications. Biomacromolecules 18(6):1736–1746CrossRef

31.

Taherimehr M, Bagheri R, Taherimehr M (2021) In-vitro evaluation of thermoplastic starch/beta-tricalcium phosphate nano-biocomposite in bone tissue engineering. Ceram Int 47(11):15458–15463CrossRef

32.

Lu DR, Xiao CM, Xu SJ (2009) Starch-based completely biodegradable polymer materials. Express Polym Lett 3(6):366–375CrossRef

33.

Hsieh W, Liau J (2013) Cell culture and characterization of cross-linked poly (vinyl alcohol) -g-starch 3D scaffold for tissue engineering. Carbohydr Polym 98(1):574–580CrossRef

34.

Mirab F, Eslamian M, Bagheri R (2018) Fabrication and characterization of a starch-based nanocomposite scaffold with highly porous and gradient structure for bone tissue engineering. Biomed Phys Eng Express 4:055021CrossRef

35.

Ramesh S, Loo ZZ, Tan CY, Chew WJ, Ching YC, Tarlochan F, Chandran H, Krishnasamy S, Bang LT, Sarhan AAD (2018) Characterization of biogenic hydroxyapatite derived from animal bones for biomedical applications. Ceram Int 44(9):10525–10530CrossRef

36.

Trinh KS, Dang TB (2019) Structural, physicochemical, and functional properties of electrolyzed cassava starch. Int J Food Sci 2019:1–8CrossRef

37.

Kokubo T, Takadama H (2006) How useful is SBF in predicting in vivo bone bioactivity? Biomaterials 27(15):2907–2915CrossRef

38.

Bee SL, Hamid ZAA (2019) Characterization of chicken bone waste-derived hydroxyapatite and its functionality on chitosan membrane for guided bone regeneration. Compos Part B Eng 163:562–573CrossRef

39.

Pramanik S, Agarwala P, Vasudevan K, Sarkar K (2020) Human-lymphocyte cell friendly starch–hydroxyapatite biodegradable composites: Hydrophilic mechanism, mechanical, and structural impact. J Appl Polym Sci 137(30):1–11CrossRef

40.

Gomaa MM, Hugenschmidt C, Dickmann M, Abdel-Hady EE, Mohamed HFM, Abdel-Hamed MO (2018) Crosslinked PVA/SSA proton exchange membranes: Correlation between physiochemical properties and free volume determined by positron annihilation spectroscopy. Phys Chem Chem Phys 20(44):28287–28299CrossRef

41.

Abdullah AHD, Chalimah S, Primadona I, Hanantyo MHG (2018) Physical and chemical properties of corn, cassava, and potato starchs. IOP Conf Ser Earth Environ Sci 160(1):2003

42.

Salgado AJ, Coutinho OP, Reis RL (2004) Bone tissue engineering: State of the art and future trends. Macromol Biosci 4(8):743–765CrossRef

43.

Karageorgiou V, Kaplan D (2005) Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 26:5474–5491CrossRef

44.

Chao H, Riggleman RA (2013) Effect of particle size and grafting density on the mechanical properties of polymer nanocomposites. Polymer 54(19):5222–5229CrossRef

45.

Liu H, Webster TJ (2010) Mechanical properties of dispersed ceramic nanoparticles in polymer composites for orthopedic applications. Int J Nanomed 5(1):299–313

46.

Zhang H, Li S, Yan Y (2001) Dissolution behavior of hydroxyapatite powder in hydrothermal solution. Ceram Int 27(4):451–454CrossRef

47.

Seol YJ, Park JY, Jung JW, Jang J, Girdhari R, Kim SW, Cho DW (2014) Improvement of bone regeneration capability of ceramic scaffolds by accelerated release of their calcium ions. Tissue Eng - Part A 20(21–22):2840–2849CrossRef

48.

Cattalini JP, Hoppe A, Pishbin F, Roether J, Boccaccini AR, Lucangioli S, Mouriño V (2015) Novel nanocomposite biomaterials with controlled copper/calcium release capability for bone tissue engineering multifunctional scaffolds. J R Soc Interface 12(110):1–13CrossRef

49.

Matthews JL, Martin JH (1971) Intracellular transport of calcium and its relationship to homeostasis and mineralization. An electron microscope study. Am J Med 50(5):589–597CrossRef

50.

Kong L, Gao Y, Lu G, Gong Y, Zhao N, Zhang X (2006) A study on the bioactivity of chitosan/nano-hydroxyapatite composite scaffolds for bone tissue engineering. Eur Polym J 42(12):3171–3179CrossRef

51.

Niu X, Fan R, Tian F, Guo X, Li P, Feng Q, Fan Y (2017) Calcium concentration dependent collagen mineralization. Mater Sci Eng C 73:137–143CrossRef

52.

Yu S, Hariram KP, Kumar R, Cheang P, Aik KK (2005) In vitro apatite formation and its growth kinetics on hydroxyapatite/polyetheretherketone biocomposites. Biomaterials 26(15):2343–2352CrossRef

53.

Cowles EA, DeRome ME, Pastizzo G, Brailey LL, Gronowicz GA (1998) Mineralization and the expression of matrix proteins during in vivo bone development. Calcif Tissue Int 62(1):74–82CrossRef

54.

Prins HJ, Braat AK, Gawlitta D, Dhert WJA, Egan DA, Tijssen-Slump E, Yuan H, Coffer PJ, Rozemuller H, Martens AC (2014) In vitro induction of alkaline phosphatase levels predicts in vivo bone forming capacity of human bone marrow stromal cells. Stem Cell Res 12(2):428–40CrossRef

55.

He C, Jin X, Ma PX (2014) Calcium phosphate deposition rate, structure and osteoconductivity on electrospun poly(l-lactic acid) matrix using electrodeposition or simulated body fluid incubation. Acta Biomater 10(1):419–427CrossRef

56.

Le TDH, Liaudanskaya V, Bonani W, Migliaresi C, Motta A (2018) Enhancing bioactive properties of silk fibroin with diatom particles for bone tissue engineering applications. J Tissue Eng Regen Med 12(1):89–97CrossRef

57.

Chiralt A, Gonz C, Cano AI (2016) Biodegradation behavior of starch-PVA films as affected by the incorporation of different antimicrobials. Polym Degrad Stab 132:11–20CrossRef

Title: Hydroxyapatite−loaded starch/polyvinyl alcohol scaffold for bone regeneration application: preparation and characterization
Authors: Thi Duy Hanh Le
Huynh Nguyen Anh Tuan
Van Tien Nguyen
Anh Thi Le
Publication date: 25-05-2023
Publisher: Springer US
Published in: Journal of Sol-Gel Science and Technology / Issue 2/2023
Print ISSN: 0928-0707
Electronic ISSN: 1573-4846
DOI: https://doi.org/10.1007/s10971-023-06137-3

Springer Professional

Abstract

Graphical Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Technik"

Springer Professional "Wirtschaft+Technik"

Other articles of this Issue 2/2023

Influence of drying technique on Pt/In2O3 aerogels for methanol steam reforming

Improved electrocaloric effect of Ba0.7Sr0.3TiO3 ceramics doped with B and Mn

Sol–gel derived iron-manganese oxide nanoparticles: a promising dual-functional material for solar photocatalysis and antimicrobial applications

Sol–gel derived Zn doped TiO2 thin films and their waveguides

Hierarchically porous and high-strength carbon aerogel-based composite for solar-driven interfacial evaporation

Nanocrystalline (HoxY1−x)2Ti2O7 luminophores for short- and mid-infrared lasers

Premium Partners