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2013 | OriginalPaper | Chapter

Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy

Authors : Sophie Brasselet, Patrick Ferrand, Alla Kress, Xiao Wang, Hubert Ranchon, Alicja Gasecka

Published in: Fluorescent Methods to Study Biological Membranes

Publisher: Springer Berlin Heidelberg

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Abstract

The use of light polarization properties in the analysis of fluorescence images has driven a large amount of research toward the measurement of orientational behavior of molecules in cells, in particular in their membranes. This field has been recently revisited to enlarge the possibilities of polarization-resolved fluorescence microscopy. We show that this technique allows retrieving a wealth of information on the constraints that hinder rotational mobility of lipid probes and proteins in membranes, bringing thus new insights on inter-proteins and lipid-protein interactions, on membrane morphology at the sub-diffraction length scale and on local membrane physical properties such as viscosity.

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Cheresh D, Leng J, Klemke R (1999) Regulation of cell contraction and membrane ruffling by distinct signals in migratory cells. J Cell Biol 146:1107–1116CrossRef

Anantharam A, Onoa B et al (2010) Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM. J Cell Biol 188:415–428CrossRef

Benninger RKP, Vanherberghen B, Onfelt B (2009) Live cell linear dichroism im-aging reveals extensive membrane ruffling within the docking structure of natural killer cell immune synapses. Biophys J 96:L13–L15CrossRef

Fooksman DR, Grönvall GK et al (2006) Clustering class I MHC modulates sensitivity of T cell recognition. J Immunol 176:6673–6680

Pentcheva T, Edidin M (2001) Clustering of peptide-loaded MHC class I molecules for endoplasmic reticulum export imaged by fluorescence resonance energy transfer. J Immunol 166:6625–6632

Borejdo J, Burlacu S (1993) Measuring orientation of actin filaments within a cell: orientation of actin in intestinal microvilli. Biophys J 65:300–309CrossRef

Brack AS, Brandmeier BD et al (2004) Bifunctional rhodamine probes of Myosin regulatory light chain orientation in relaxed skeletal muscle fibers. Biophys J 86:2329–2341CrossRef

Vrabioiu AM, Mitchison TJ (2006) Structural insights into yeast septin organization from polarized fluorescence microscopy. Nature 443:466–469CrossRef

Weber G (1953) Rotational Brownian motion and polarization of the fluorescence of solutions. Adv Protein Chem 8:415–459CrossRef

10.

Cantor CR, Schimmel PR (1980) Biophysical chemistry, part 2: techniques for the study of biological structure and function. W. H. Freeman, San Francisco

11.

Chan FTS, Kaminski CF, Kaminski Schierle GS (2011) HomoFRET fluorescence anisotropy imaging as a tool to study molecular self-assembly in live cells. Chem Phys Chem 12:500–509CrossRef

12.

Schlessinger J, Koppel DE et al (1976) Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts. Proc Natl Acad Sci USA 73:2409–2413CrossRef

13.

Schwille P, Haupts U et al (1999) Molecular dynamics in living cells observed by fluorescence correlation spectroscopy with one- and two-photon excitation. Biophys J 77:2251–2265CrossRef

14.

Schütz GJ, Kada G et al (2000) Properties of lipid microdomains in a muscle cell membrane visualized by single molecule microscopy. EMBO J 19:892–901CrossRef

15.

Oida T, Sako Y, Kusumi A (1993) Fluorescence lifetime imaging microscopy (flimscopy). Methodology development and application to studies of endosome fusion in single cells. Biophys J 64:676–685CrossRef

16.

Manders EEM, Verbeek FJ, Aten JA (1993) Measurement of co-localisation of objects in dual-colour confocal images. J Microsc 169:375–382CrossRef

17.

Gould TJ, Gunewardene MS, Gudheti MV, Verkhusha VV, Yin SR, Gosse JA, Hess ST (2008) Nanoscale imaging of molecular positions and anisotropies. Nat Methods 5:1027–1030CrossRef

18.

Lazar J, Bondar A, Timr S, Firestein SJ (2011) Two-photon polarization microscopy reveals protein structure and function. Nat Methods 8:684–690CrossRef

19.

Kress A, Ferrand P, Rigneault H, Trombik T, He HT, Marguet D, Brasselet S (2011) Probing MHC class I protein and lipid order in cell membranes by fluorescence polarisation-resolved imaging. Biophys J 101:468–476CrossRef

20.

Marguet D, Spiliotis ET et al (1999) Lateral diffusion of GFP-tagged H2Ld molecules and of GFP-TAP1 reports on the assembly and retention of these molecules in the endoplasmic reticulum. Immunity 11:231–240CrossRef

21.

Rocheleau JV, Edidin M, Piston DW (2003) Intrasequence GFP in class I MHC molecules, a rigid probe for fluorescence anisotropy measurements of the membrane environment. Biophys J 84:4078–4086CrossRef

22.

Griffin BA, Adams SR, Tsien RY (1998) Specific covalent labeling of recombinant protein molecules inside live cells. Science 281:269–272CrossRef

23.

Spille JH, Zürn A, Hoffmann C, Lohse MJ, Harms GS (2011) Rotational diffusion of the α2a adrenergic receptor revealed by FlAsH labeling in living cells. Biophys J 100:1139–1148CrossRef

24.

Axelrod D (1979) Carbocyanine dye orientation in red cell membrane studied by microscopic fluorescence polarization. Biophys J 26:557–573CrossRef

25.

Florine-Casteel K (1990) Phospholipid order in gel- and fluid-phase cell-size liposomes measured by digitized video fluorescence polarization microscopy. Biophys J 57:1199–1215CrossRef

26.

Benninger RKP, Onfelt B et al (2005) Fluorescence imaging of two-photon linear dichroism: cholesterol depletion disrupts molecular orientation in cell membranes. Biophys J 88:609–622CrossRef

27.

Haluska CK, Schröder AP et al (2008) Combining fluorescence life-time and polarization microscopy to discriminate phase separated domains in giant unilamellar vesicles. Biophys J 95:5737–5747CrossRef

28.

Brasselet S (2011) Polarization resolved nonlinear microscopy: application to structural molecular and biological imaging. Adv Opt Photonics 3:205–271CrossRef

29.

Ikonen E (2001) Roles of lipid rafts in membrane transport. Curr Opin Cell Biol 13:470–477CrossRef

30.

McConnell HM, Vrljic M (2003) Liquid-liquid immiscibility in membranes. Annu Rev Biophys Biomol 32:469–492. 24

31.

Munro S (2003) Lipid rafts: elusive or illusive? Cell 115:377–388. 26

32.

Simons K, Ikonen E (1997) Functional rafts in cell membranes. Nature 387:569–572CrossRef

33.

Veatch SL, Keller SL (2002) Organization in lipid membranes containing cholesterol. Phys Rev Lett 89:268101CrossRef

34.

Lakowicz JR (1999) Principles of fluorescence spectroscopy. Springer, New York

35.

Perrin F (1926) La fluorescence des solutions. Polarisation. Vie moyenne des molécules dans l’état excité. J de Phys 7:390–401

36.

Dix JA, Verkman AS (1990) Mapping of fluorescence anisotropy in living cells by ratio imaging. Biophys J 57:231–240CrossRef

37.

Bader AN, Hofman EG, Voortman J, van Bergen en Henegouwen PMP, Gerritsen HC (2009) Homo-FRET imaging enables quantification of protein cluster sizes with subcellular resolution. Biophys J 97:2613–2622CrossRef

38.

Foster TH, Pearson BD, Mitra S, Bigelow CE (2005) Fluorescence anisotropy imaging reveals localization of meso-tetrahydroxyphenyl chlorin in the nuclear envelope. Photochem Photobiol 81:1544–1547CrossRef

39.

Clegg RM (1996) Fluorescence resonance energy transfer. In: Fluorescence imaging spectroscopy and microscopy, vol 137, 13th edn, Chemical analysis. Wiley, New York

40.

Patterson GH, Piston DW, Barisas BG (2000) Förster distances between green fluorescent protein pairs. Anal Biochem 284:438–440CrossRef

41.

Blackman SM, Piston DW, Beth AH (1998) Oligomeric state of human erythrocyte band 3 measured by fluorescence resonance energy homotransfer. Biophys J 75:1117–1130CrossRef

42.

Gautier I, Tramier M et al (2001) Homo-FRET microscopy in living cells to measure monomer-dimer transition of GFP-tagged proteins. Biophys J 80:3000–3008CrossRef

43.

Varma R, Mayor S (1998) GPI-anchored proteins are organized in submicron domains at the cell surface. Nature 394:798–801CrossRef

44.

Gasecka A, Han T-J et al (2009) Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry. Biophys J 97:2854–2862CrossRef

45.

Dale RE, Hopkins SC et al (1999) Model-independent analysis of the orientation of fluorescent probes with restricted mobility in muscle fibers. Biophys J 76:1606–1618CrossRef

46.

Kinosita K, Kawato S, Ikegami A (1977) A theory of fluorescence polarization decay in membranes. Biophys J 20:289–305CrossRef

47.

Blackman SM, Cobb CE et al (1996) The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy. Biophys J 71:194–208CrossRef

48.

Mattheyses AL, Kampmann M et al (2010) Fluorescence anisotropy reveals order and disorder of protein domains in the nuclear pore complex. Biophys J 99:1706–1717CrossRef

49.

Barda-Saad M, Braiman A et al (2005) Dynamic molecular interactions linking the T cell antigen receptor to the actin cytoskeleton. Nat Immunol 6:80–89CrossRef

50.

Bunnell SC, Kapoor V et al (2001) Dynamic actin polymerization drives T cell receptor-induced spreading: a role for the signal transduction adaptor LAT. Immunity 14:315–329CrossRef

51.

Edidin M, Kuo SC, Sheetz MP (1991) Lateral movements of membrane glyco-proteins restricted by dynamic cytoplasmic barriers. Science 254:1379–1382CrossRef

52.

Edidin M, Zuniga MC, Sheetz MP (1994) Truncation mutants define and locate cytoplasmic barriers to lateral mobility of membrane glycoproteins. Proc Natl Acad Sci USA 91:3378–3382CrossRef

53.

Treanor B, Depoil D et al (2010) The membrane skeleton controls diffusion dynamics and signaling through the B cell receptor. Immunity 32:187–199CrossRef

54.

Schauer K, Duong T et al (2010) Probabilistic density maps to study global endomembrane organization. Nat Methods 7:560–566CrossRef

55.

Kress A, Wang X, Ranchon H, Ferrand P, Brasselet S (2012) Revealing molecular orientation and order in cell membranes of arbitrary shape (submitted)

56.

Brasselet S, LeFloc’h V, Treussart F, Roch J, Zyss J (2004) In situ diagnostics of the crystalline nature of single organic nanocrystals by nonlinear microscopy. Phys Rev Lett 92:207401CrossRef

57.

LeFloc’h V (2003) Monitoring of orientation in molecular ensembles by polarization sensitive nonlinear microscopy. J Phys Chem B 107:12403–12410CrossRef

58.

Schön P, Munhoz F et al (2008) Polarization distortion effects in polarimetric two-photon microscopy. Opt Express 16:20891–20901CrossRef

59.

Richards B, Wolf E (1959) Electromagnetic diffraction in optical systems. II. Structure of the image field in an aplanatic system. Proc Roy Soc Lond A 253:358–379CrossRef

60.

Schön P, Behrndt M, Ait-Belkacem D, Rigneault H, Brasselet S (2010) Polarization and phase pulse shaping applied to structural contrast in nonlinear microscopy imaging. Phys Rev A 81:013809CrossRef

61.

Moyano F, Biasutti MA, Silber JJ, Correa NM (2006) New insights on the behavior of Prodan in homogeneous media and in large unilamellar vesicles. J Phys Chem B 110:11838–11846CrossRef

62.

Muller JM, Harryvan DH, Verhagen JCD, van Faassen EE, van Ginkel G (1996) The orientation of the transition dipole moments of TMADPH embedded in a poly(vinyl alcohol) film. Chem Phys 211:413–420CrossRef

63.

Baumgart T, Hess ST, Webb WW (2003) Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension. Nature 425:821–824CrossRef

64.

Dietrich C, Bagatolli LA, Volovyk ZN, Thompson NL, Levi M (2001) Lipid rafts reconstituted in model membranes. Biophys J 80:1417–1428CrossRef

65.

Samsonov AV, Mihalyov I, Cohen FS (2001) Characterization of cholesterol-sphingomyelin domains and their dynamics in bilayer membranes. Biophys J 81:1486–1500CrossRef

66.

Scherfeld D, Kahya N, Schwille P (2003) Lipid dynamics and domain formation in model membranes composed of ternary mixtures of unsaturated and saturated phosphatidylcholines and cholesterol. Biophys J 85:3758–3768CrossRef

67.

Bagatolli LA, Gratton E (1999) Two-photon fluorescence microscopy observation of shape changes at the phase transition in phospholipid giant unilamellar vesicles. Biophys J 77:2090–2101CrossRef

68.

Baumgart T, Hunt G, Farkas ER, Webb WW, Feigenson GW (2007) Fluorescence probe partitioning between Lo/Ld phases in lipid membranes. Biochim Biophys Acta 1768:2182–2194CrossRef

69.

Kim HM, Choo H-J, Jung S-Y, Ko Y-G, Park W-H (2007) A two-photon fluorescent probe for lipid raft imaging: C-laurdan. Chembiochem 8:553–559CrossRef

70.

Gidwani A, Holowka D, Baird B (2001) Fluorescence anisotropy measurements of lipid order in plasma membranes and lipid rafts from RBL-2 H3 mast cells. Biochemistry 40:12422–12429CrossRef

71.

Farkas ER, Webb WW (2010) Multiphoton polarization imaging of steady-state molecular order in ternary lipid vesicles for the purpose of lipid phase assignment. J Phys Chem B 114:15512–15522CrossRef

72.

van Rheenen J, Jalink K (2002) Agonist-induced PIP(2) hydrolysis inhibits cortical actin dynamics: regulation at a global but not at a micrometer scale. Mol Biol Cell 13:3257–3267CrossRef

73.

Chong PLG, Wong PTT (1993) Interactions of Laurdan with phosphatidylcholine liposomes: a high pressure FTIR study. Biochim Biophys Acta 1149:260–266CrossRef

74.

Adler J, Shevchuk AI et al (2010) Plasma membrane topography and interpretation of single-particle tracks. Nat Methods 7:170–171CrossRef

75.

Sund SE, Swanson JA, Axelrod D (1999) Cell membrane orientation visualized by polarized total internal reflection fluorescence. Biophys J 77:2266–2283CrossRef

Title: Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy
Authors: Sophie Brasselet
Patrick Ferrand
Alla Kress
Xiao Wang
Hubert Ranchon
Alicja Gasecka
Publisher: Springer Berlin Heidelberg
Book: Fluorescent Methods to Study Biological Membranes
Print ISBN: 978-3-642-33127-5

Electronic ISBN: 978-3-642-33128-2

Copyright Year: 2013
DOI: https://doi.org/10.1007/4243_2012_51

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