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2013 | OriginalPaper | Buchkapitel

Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy

verfasst von : Sophie Brasselet, Patrick Ferrand, Alla Kress, Xiao Wang, Hubert Ranchon, Alicja Gasecka

Erschienen in: Fluorescent Methods to Study Biological Membranes

Verlag: Springer Berlin Heidelberg

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Abstract

The use of light polarization properties in the analysis of fluorescence images has driven a large amount of research toward the measurement of orientational behavior of molecules in cells, in particular in their membranes. This field has been recently revisited to enlarge the possibilities of polarization-resolved fluorescence microscopy. We show that this technique allows retrieving a wealth of information on the constraints that hinder rotational mobility of lipid probes and proteins in membranes, bringing thus new insights on inter-proteins and lipid-protein interactions, on membrane morphology at the sub-diffraction length scale and on local membrane physical properties such as viscosity.

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Vorheriges Kapitel STED-FCS Nanoscopy of Membrane Dynamics

Nächstes Kapitel Near-Field Optical Nanoscopy of Biological Membranes

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Titel: Imaging Molecular Order in Cell Membranes by Polarization-Resolved Fluorescence Microscopy
verfasst von: Sophie Brasselet
Patrick Ferrand
Alla Kress
Xiao Wang
Hubert Ranchon
Alicja Gasecka
Verlag: Springer Berlin Heidelberg
Buch: Fluorescent Methods to Study Biological Membranes
Print ISBN: 978-3-642-33127-5

Electronic ISBN: 978-3-642-33128-2

Copyright-Jahr: 2013
DOI: https://doi.org/10.1007/4243_2012_51

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