Top

Medical & Biological Engineering & Computing

Published in:

01-02-2013 | Original Article

In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells

Authors: Niina Onnela, Leena Lehtonen, Mikko Koski, Jari Hyttinen

Published in: Medical & Biological Engineering & Computing | Issue 1-2/2013

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

The purpose of this study is to develop and test a method to reveal if the retinal pigment epithelium (RPE) cells differentiated from human embryonic stem cells (hESC) support the functions of photoreceptors. hESC-derived RPE (hESC–RPE) cells offer a potent cell source for cell replacement therapy that may be used to prevent certain eye diseases. Methods to assure the functionality of the RPE cells are well warranted. Electroretinograms (ERG) measure the electrophysiological response of the retina to light stimuli. A setup was developed that enables the measurement of ERG in vitro from mice retinas cultured together with hESC–RPE cells. The co-culture of RPE and retinas seems to be a viable tool to assess the functionality of RPE in vitro. However, owing to limited sample size results were somewhat mixed, and thus it was not possible to prove that hESC–RPE cells enhance the ERG response of a mouse retina in vitro. The long-term culturing of the retinas needs to be refined to acquire more conclusive evidence of the supporting role of the RPE and to explore the full potential of the co-culture and ERG methods in assessing RPE functionality.

previous article Wavelet-based sparse functional linear model with applications to EEGs seizure detection and epilepsy diagnosis

next article Off-line determination of the optimal number of iterations of the robust anisotropic diffusion filter applied to denoising of brain MR images

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Wirtschaft"

Online-Abonnement

Mit Springer Professional "Wirtschaft" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 340 Zeitschriften

aus folgenden Fachgebieten:

Bauwesen + Immobilien
Business IT + Informatik
Finance + Banking
Management + Führung
Marketing + Vertrieb
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Carr A-J, Vugler AA, Hikita ST, Lawrence JM, Gias C, Chen LL, Buchholz DE, Ahmado A, Semo M, Smart MJK, Hasan S, da Cruz L, Johnson LV, Clegg DO, Coffey PJ (2009) Protective effects of human iPS-derived retinal pigment epithelium cell transplantation in the Retinal Dystrophic Rat. Plos One 4(12):e8152PubMedCrossRef

Coffey PJ, Girman S, Wang SM, Hetherington L, Keegan DJ, Adamson P, Greenwood J, Lund RD (2002) Long-term preservation of cortically dependent visual function in RCS rats by transplantation. Nat Neurosci 5:53–56PubMedCrossRef

da Cruz L, Chen FK, Ahmado A, Greenwood J, Coffey P (2007) RPE transplantation and its role in retinal disease. Prog Retin Eye Res 26:598–635PubMedCrossRef

Dunn KC, Aotaki-Keen AE, Putkey FR, Hjelmeland LM (1996) ARPE19, a human retinal pigment epithelial cell line with differentiated properties. Exp Eye Res 59:587–596

Eleftheriou CG, Zimmermann J, Kjeldsen H, Pur MD, Hanein Y, Serganor E (2012) Towards the development of carbon nanotube-based retinal implant technology: electrophysiological and ultrastructural evidence of coupling at the biohybrid interface. In: Stett A, Zeck G (eds) Proceedings of the 8th int. meeting on substrate-integrated microelectrode arrays. NMI Natural and Medical Sciences Institute, University of Tuebingen, Reutlingen, 2012

Falkner-Radler C, Krebs I, Glittenberg C, Považay B, Drexler W, Graf A, Binder S (2011) Human retinal pigment epithelium (RPE) transplantation: outcome after autologous RPE-choroid sheet and RPE cell-suspension in a randomised clinical study. Br J Ophthalmol 95:370–375PubMedCrossRef

Frambach DA, Fain GL, Farber DB, Bok D (1990) Beta adrenergic receptors on cultured human retinal pigment epithelium. Investig Ophthalmol Vis Sci 31:1767–1772

Haruta M, Sasai Y, Kawasaki H, Amemiya K, Ooto S, Kitada M, Suemori H, Nakatsuji N, Ide C, Honda Y, Takahashi M (2004) In vitro and in vivo characterization of pigment epithelial cells differentiated from primate embryonic stem cells. Investig Ophthalmol Vis Sci 45:1020–1025CrossRef

Idelson M, Alper R, Obolensky A, Ben-Shushan E, Hemo I, Yachimovich-Cohen N, Khaner H, Smith Y, Wiser O, Gropp M, Cohen MA, Even-Ram S, Berman-Zaken Y, Matzrafi L, Rechavi G, Banin E, Reubinoff B (2009) Directed differentiation of human embryonic stem cells into functional retinal pigment epithelium cells. Cell Stem Cell 5:396–408PubMedCrossRef

10.

Johnson TV, Martin KR (2008) Development and characterization of an adult retinal explant organotypic tissue culture system as an in vitro intraocular stem cell transplantation model. Investig Ophthalmol Vis Sci 49:3503–3512CrossRef

11.

Kaempf S, Walter P, Salz AK, Thumann G (2008) Novel organotypic culture model of adult mammalian neurosensory retina in co-culture with retinal pigment epithelium. J Neurosci Methods 173:47–58PubMedCrossRef

12.

Kaufman PL, Aml A (2003) Adler’s physiology of the eye—clinical application, 10th edn. Mosby, USA

13.

Klimanskaya I, Hipp J, Rezai KA, West M, Atala A, Lanza R (2004) Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics. Clone Stem Cells 6:217–245

14.

Kobuch K, Herrmann WA, Framme C, Sachs HG, Gabel V-P, Hillenkamp J (2008) Maintenance of adult porcine retina and retinal pigment epithelium in perfusion culture: characterisation of an organotypic in vitro model. Exp Eye Res 86:661–668PubMedCrossRef

15.

Lund D, Wang S, Klimanskaya I, Holmes T, Ramos-Kelsey R, Lu B, Girman S, Bischoff N, Sauvé Y, Lanza R (2006) Human embryonic stem cell-derived cells rescue visual function in dystrophic RCS rats. Clone Stem Cells 8:189–199CrossRef

16.

Maminishkis A, Chen S, Jalickee S, Banzon T, Shi G, Wang FE, Ehalt T, Hammer AJ, Miller SS (2006) Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue. Investig Ophthalmol Vis Sci 47:3612–3624CrossRef

17.

Mannermaa E, Reinisalo M, Ranta VP, Vellonen KS, Kokki H, Saarikko A, Kaarniranta K, Urtti A (2010) Filter-cultured ARPE-19 cells as outer blood-retinal barrier model. Eur J Pharm Sci 40:289–296PubMedCrossRef

18.

Monaim MA, Suleiman JH, Ashraf M (2005) Morphological recovery in the reattached retina of the toad Bufo marinus: a new experimental model of retinal detachment. Arch Med Res 36:107–112CrossRef

19.

Nevala H, Ylikomi T, Tähti H (2008) Evaluation of the selected barrier properties of retinal pigment epithelial cell line ARPE-19 for an in vitro blood-barrier model. Hum Exp Toxicol 27:741–749PubMedCrossRef

20.

Nusinowitz S, Ridder WH, Heckenlively JR (2002) Electrophysiological testing of the mouse visual system. Systematic evaluation of the mouse eye: anatomy, pathology, and biomethods. Smith R (eds), CRC Press, Boca Raton

21.

Onnela N, Savolainen V, Juuti-Uusitalo K, Vaajasaari H, Skottman H, Hyttinen J (2011) Electric impedance of human embryonic stem cell derived retinal pigment epithelium. Med Biol Eng Comput 50:107–116PubMedCrossRef

22.

Rajasekaran SA, Hu J, Gopal J, Gallemore R, Ryazantsev S, Bok D, Rajasekaran AK (2003) Na, K-ATPase inhibition alters tight junction structure and permeability in human retinal pigment epithelial cells. Am J Physiol Cell Physiol 284:C1497–C1507PubMed

23.

Roth F, Bindewald A, Holz FG (2004) Key pathophysiologic pathways in age-related macular disease. Greafe’s Arch Clin Exp Ophthalmol 242:710–716CrossRef

24.

Savolainen V, Juuti-Uusitalo K, Onnela N, Vaajasaari H, Narkilahti S, Suuronen R, Skottman H, Hyttinen J (2011) Impedance spectroscopy in monitoring the maturation of stem cell-derived retinal pigment epithelium. Ann Biom Eng 39:3055–3069CrossRef

25.

Schwartz SD, Hubschman J-P, Heilwell G, Franco-Cardenas V, Pan CK, Otsrick RM, Mickunas E, Gay R, Klimanskaya I, Lanza R (2012) Embryonic stem cell trials for macular degeneration: a preliminary report. Lancet 379:713–720PubMedCrossRef

26.

Skottman H (2009) Derivation and characterization of three new human embryonic stem cell lines in Finland. In Vitro Cell Dev Biol Anim 46:206–209CrossRef

27.

Strauss O (2005) The retinal pigment epithelium in visual function. Physiol Rev 85:845–881PubMedCrossRef

28.

Toimela T, Mäenpää H, Mannerström M, Tähti H (2004) Development of an in vitro blood-barrier model-cytotoxicity of mercury and aluminum. Toxicol Appl Pharmacol 195:73–82PubMedCrossRef

29.

Vaajasaari H, Ilmarinen T, Juuti-Uusitalo K, Rajala K, Onnela N, Narkilahti S, Suuronen R, Hyttinen J, Uusitalo H, Skottman H (2011) Towards defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells. Mol Vis 17:558–575PubMed

30.

Wu J, Peachey NS, Marmorstein AD (2004) Light-evoked responses of the mouse retinal pigment epithelium. J Neurophysiol 91:1134–1142PubMedCrossRef

Title: In vitro electroretinogram for the study of the functionality of differentiated retinal pigment epithelium cells
Authors: Niina Onnela
Leena Lehtonen
Mikko Koski
Jari Hyttinen
Publication date: 01-02-2013
Publisher: Springer-Verlag
Published in: Medical & Biological Engineering & Computing / Issue 1-2/2013
Print ISSN: 0140-0118
Electronic ISSN: 1741-0444
DOI: https://doi.org/10.1007/s11517-012-0968-7

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Springer Professional "Wirtschaft"

Other articles of this Issue 1-2/2013

A dynamic Bayesian network for estimating the risk of falls from real gait data

Using three-dimensional multigrid-based snake and multiresolution image registration for reconstruction of cranial defect

Deriving respiration from photoplethysmographic pulse width

Effect of arm position on the prediction of kinematics from EMG in amputees

A hybrid reconstruction algorithm for fluorescence tomography using Kirchhoff approximation and finite element method

A quasi-brittle continuum damage finite element model of the human proximal femur based on element deletion

Premium Partner