2.3 Measurements and analyte sampling equipment
The air quality screening studies performed in an enclosed area were carried out with the use of Radiello® diffusive passive samplers (Fondazione Salvatore Maugeri, Padova, Italy). The application of a passive sampling technique facilitates collection of the analytes from the gaseous phase without any additional equipment such as aspirators, pumps, or additional gas wires, which allows performing the research without disturbing the regular use of defined enclosed area or working place. Detailed information about the characteristics and technical parameters of the Radiello® diffusive passive sampler is listed elsewhere (Marć et al.
2014a,
b; Plaisance et al.
2008). In brief, the exposure time of applied passive samplers in enclosed space was set up to 4 h. The short sampling period was associated mainly with the indoor environment conditions (the possibility of high or very high content level of organic compounds in gaseous phase due to the specific character of the indoor sampling area that might “overload” the sorption medium installed inside the diffusive porous polyethylene membrane). Each time, two independent Radiello® passive samplers were installed, at a distance of more than 1 m to ensure optimal conditions for collecting analytes from the gaseous phase (adequate air circulation around the passive sampler, as well as avoiding the phenomenon of competitiveness between installed passive samplers). The passive samplers were installed very close to the workplace and in close vicinity to the materials preparation area. During the passive sampling working period, the temperature inside the studied area was constantly monitored and was in the range from 21.1 up to 23.1 °C. After the sampling period, the cylindrical tubes with sorption medium (Carbotrap 4) were removed from the diffusive membrane and enclosed in the glass containers. Next, the sorption medium with adsorbed analytes was transported to the laboratory and stored at a temperature of 4 °C no longer than 12 h after sampling. The identification of the main volatile organic compounds in the air of the enclosed space was performed with the use of thermal desorption technique (Unity v.2, Markes International Ltd., Pontyclun, UK), connected with gas chromatography (Agilent Technologies 6890) combined with a mass spectrometer (5873 Network Mass Selective Detector, Agilent Technologies). The cylindrical containers filled with sorption medium were desorbed at a temperature of 290 °C for 15 min. During this process, the analytes were transported to the microtrap. Then, the analytes were liberated from the microtrap (ballistic heating up to 300 °C and maintained for 5 min) and transported by the helium flow (1.5 mL min
−1) to the GC capillary column (Agilent 122-5563, J&W DB-5MS, 60 m × 0.25 mm × 1 µm). The GC oven temperature program was as follows: 50 °C for 1 min, then raised at a rate of 10 °C min
−1 up to 280 °C and maintained for 10 min. The temperature of the TD-GC transfer line was set to 150 °C, and the temperature of the GC–MS transfer line was 280 °C. The employed mass spectrometer was working in a SCAN mode. Detailed information about the principals and characteristic of the thermal desorption technique is presented elsewhere (Król et al.
2012; Zabiegała et al.
2007). To eliminate signals associated with other processes occurring in the laboratory hall and select only signals related to the performed modification, as well as to ensure the reliability of obtained results, the “background” was also analyzed. Additionally, before each sampling period, the sorption medium placed in the cylindrical tube was conditioned for 30 min at a temperature of 300 °C.
The emission studies (expressed by TVOCs parameter) were performed using the microscale stationary emission chamber system (Markes International Micro-Chamber/Thermal Extractor M-CTE250) consisting of four stainless steel chambers with the internal volume of 114 cm
3. Detailed information about the characteristic of the stationary emission chamber system is described elsewhere (Marć and Zabiegała
2017; Marć et al.
2017). In brief, the sample of modified lignocellulosic fillers (mass of a sample—1.073 ± 0.059 g) was firstly placed on a Petri glass dish and then installed inside the chamber to prevent chamber contamination with small particles of solid samples. Next, the chambers were tightly closed, and at the head of the cover, the stainless steel tube filled with sorption medium Tenax TA was installed to collect the analytes emitted from the surface of the studied samples to the gaseous phase. Then, the sampling conditions were set: samples seasoning time—20 min; inert gas (nitrogen) flow rate—15 mL min
−1; seasoning temperature—40 °C. After the sampling process, the stainless steel tubes were removed and transferred directly to the thermal desorber (Mareks Int, Unity 2) connected with gas chromatograph (Agilent Technologies 7820A) combined with flame ionization detector. The stainless steel tubes filled with Tenax TA were desorbed at a temperature of 285 °C for 12 min. During this process, the analytes were transported to the microtrap. Then, similar to the TD-GC–MS system, the analytes were liberated from the microtrap (ballistic heating up to 300 °C and maintained for 5 min) and transported by the helium flow (2.0 mL min
−1) to the GC capillary column (J&W, DB-1, 30 m × 0.32 mm × 5 µm). The GC oven temperature program was as follows: 45 °C for 1 min, then raised at a rate of 15 °C min
−1 up to 120 °C maintained for 2 min, and then raised at a rate of 10 °C up to 250 °C and held for 5 min. The temperature of the TD-GC transfer line was set to 160 °C, and the FID temperature was 250 °C. Before every sampling period, the chambers with the glass Petri dish inlet were conditioned at a temperature of 100 °C for 30 min, and the blank sample (background value) was measured to ensure the reliability of the obtained results. Additionally, before each sampling period, the Tenax TA stainless steel tubes were conditioned for 30 min at a temperature of 300 °C. The most important information about the calibration process of the mentioned TD-GC-FID system and the assessment of the basic validation parameters is described in detail in previous papers (Marć and Zabiegała
2017; Marć et al.
2017). The values of the TVOC parameter were assessed based on the toluene equivalent. The recovery values of toluene from the applied Tenax TA sorption resin ranged from 95 up to 105%. As for the LOD, the value of this parameter was estimated based on the characteristic of the prepared calibration curve. The calculated value of LOD for toluene was 0.040 ng, and the LOQ value (3 × LOD) was 0.12 ng.
The particle size distribution of modified lignocellulosic fillers was characterized using a laser particle sizer Fritsch ANALYSETTE 22 apparatus operated in the range of 0.08–2000 µm.
Scanning electron microscopy (SEM) was applied in order to evaluate the changes on the fillers’ surface as a result of performed modifications. The scanning electron microscope (SEM)—model MIRA3—from Tescan (Brno, Czech Republic), was used to assess the structure of the external and internal surfaces of the rotationally molded products. The structures of the surfaces of the rotationally molded samples were assessed at an accelerating voltage of 1 kV.
The chemical structure of lignocellulosic fillers’ samples was determined using Fourier transform infrared spectroscopy (FTIR) analysis performed by a Nicolet Spectrometer IR200 from Thermo Scientific (USA). The device had ATR attachment with a diamond crystal. Measurements were taken with 1 cm−1 resolution in the range from 4000 to 400 cm−1 and 64 scans.
The color of ground organic powders was evaluated according to the Commission Internationale de l’Eclairage (CIE) through L*a*b* coordinates (International Commission on Illumination
1978). In this system, L* is the color lightness (L* = 0 for black and L* = 100 for white), a* is the green (−)/red (+) axis, and b* is the blue (−)/yellow (+) axis. Thirty tests of each sample were done and used for the determination of arithmetic mean values. The color was determined by optical spectroscopy using HunterLab Miniscan MS/S-4000S spectrophotometer, placed additionally in a specially designed light trap chamber. The total color difference parameter (ΔE*) was calculated according to the following formulation (1) (Bociąga and Trzaskalska
2016):
$$\Delta E^{*} = \left[ {\left( {\Delta L^{*} } \right)^{2} + \left( {\Delta a^{*} } \right)^{2} + \left( {\Delta b^{*} } \right)^{2} } \right]^{0.5}$$
(1)
The thermal analysis was performed using the TG 209 F3 apparatus from Netzsch (Germany). Samples of fillers weighing approximately 10 mg were placed in a ceramic dish. The study was conducted in an inert gas atmosphere—nitrogen in the range from 30 to 900 °C with a temperature increase rate of 10 °C min−1. Two specimens were analyzed for each sample.