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Cellulose

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01-04-2011

Isolation and characterization of the cellulose synthase genes PpCesA6 and PpCesA7 in Physcomitrella patens

Authors: Hua Zhang Wise, Inder M. Saxena, R. Malcolm Brown Jr.

Published in: Cellulose | Issue 2/2011

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Abstract

Analysis of cellulose biosynthesis using molecular approaches has been successful in identifying genes in many cellulose-producing organisms, yet the mechanism of cellulose biosynthesis still remains to be understood. We are interested in developing the moss Physcomitrella patens as a useful system for the study of cellulose biosynthesis. This moss affords a number of advantages including a haploid dominated gametophyte and a very high efficiency of homologous recombination in its nuclear DNA for constructing gene knockouts. In addition, P. patens has only a primary cell wall unlike Arabidopsis thaliana, which has both a primary and a secondary cell wall. We identified two full-length cellulose synthase (CesA) genes of P. patens, PpCesA6 and PpCesA7 from an EST database and have analyzed the genomic sequences. PpCesA6 and PpCesA7 show high similarity to each other, both at the cDNA and genomic DNA levels. Single and double knockouts of PpCesA6 and PpCesA7 were generated and screened for phenotypic changes. While the PpCesA6 and PpCesA7 single knockouts did not show any obvious phenotypic differences from the wild-type, the double knockout had significantly reduced stem length. These results suggest that PpCesA6 and PpCesA7 probably have a very similar role in cellulose biosynthesis and their functions may be redundant. Additionally, their roles may overlap with the other P. patens CesAs as observed for CesAs involved in primary cell wall biosynthesis in A. thaliana.

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Appenzeller L, Doblin M, Barreiro R, Wang HY, Niu XM, Kollipara K, Carrigan L, Tomes D, Chapman M, Dhugga KS (2004) Cellulose synthesis in maize: isolation and expression analysis of the cellulose synthase (CesA) gene family. Cellulose 11:287–299CrossRef

Arioli T, Peng L, Betzner AS, Burn J, Wittke W, Herth W, Camilleri C, Hofte H, Plazinski J, Birch R, Cork A, Glover J, Redmond J, Williamson RE (1998) Molecular analysis of cellulose biosynthesis in Arabidopsis. Science 279:717–720CrossRef

Ashton NW, Champagne CEM, Weiler T, Verkoczy LK (2000) The bryophyte Physcomitrella patens replicates extrachromosomal transgenic elements. New Phytol 146:391–402CrossRef

Blanton RL, Fuller D, Iranfar N, Grimson MJ, Loomis WF (2000) The cellulose synthase gene of Dictyostelium. Proc Natl Acad Sci USA 97:391–2396CrossRef

Brown RM Jr, Montezinos D (1976) Cellulose microfibrils: visualization of biosynthetic and orienting complexes in association with the plasma membrane. Proc Natl Acad Sci USA 73:143–147CrossRef

Brown RM Jr (1990) Algae as tools in studying the biosynthesis of cellulose: nature’s most abundant macromolecule. In: Wiessner W, Robinson DG, Starr RC (eds) Experimental phycology: cell walls and surfaces, reproduction, photosynthesis. Spring-Verlag, New York, pp 19–39

Brown RM Jr, Willison JHM, Richardson CL (1976) Cellulose biosynthesis in Acetobater xylium: visualization of the site of synthesis and direct measurement of the in vivo process. Proc Natl Acad Sci USA 73:4565–4569CrossRef

Carpita NC, Gibeaut DM (1993) Structural models of the primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 3:1–30CrossRef

Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J (1996) Engineered GFP as a vital reporter in plants. Curr Biol 6:325–330CrossRef

Cove DJ, Knight CD, Lamparter T (1997) Mosses as model systems. Trends Plant Sci 2:99–105CrossRef

Desprez T, Vernhettes S, Fagard M, Refregier G, Desnos T, Aletti E, Pelletier N, Py S, Hofte H (2002) Resistance against herbicide isoxaben and cellulose deficiency caused by distinct mutations in same cellulose synthase isoform CESA6. Plant Physiol 128:482–490CrossRef

Desprez T, Juraniec M, Crowell EF, Jouy H, Pochylova Z, Parcy F, Hofte H, Gonneau M, Vernhettes S (2007) Organization of cellulose synthase complexes involved in primary cell wall synthesis in Arabidopsis thaliana. Proc Natl Acad Sci USA 104:15572–15577CrossRef

Djerbi S, Lindskog M, Arvestad L, Sterky F, Teeri TT (2005) The genome sequence of black cottonwood (Populus trichocarpa) reveals 18 conserved cellulose synthase (CesA) genes. Planta 221:739–746CrossRef

Franz G, Blaschek W (1990) Cellulose. In: Dey PM, Harborne JB (eds) Methods in plants biochemistry, vol II. Carbohydrates. Academic Press, London, pp 291–322

Gardiner JC, Taylor NG, Turner SR (2003) Control of cellulose synthase complex localization in developing xylem. Plant Cell 15:1740–1748CrossRef

Geisler-Lee J, Geisler M, Coutinho PM, Segerman B, Nishikubo N, Takahashi J, Aspeborg H, Djerbi S, Master E, Andersson-Gunneras S, Sundberg B, Karpinski S, Teeri TT, Kleczkowski LA, Henrissat B, Mellerowicz EJ (2006) Poplar carbohydrate-active enzymes. Gene identification and expression analyses. Plant Physiol 140:946–962CrossRef

Grimsley NH, Ashton NW, Cove DJ (1977) The production of somatic hybrids by protoplast fusion in the moss, Physcomitrella patens. Mole Gen Genet 154:97–100CrossRef

Hara K, Morita M, Takahashi R, Sugita M, Kato S, Aoki S (2001) Characterization of two genes, Sig1 and Sig2, encoding distinct plastid ơ factor in the moss Physcomitrella patens: phylogenetic relationships to plastid σ factors in higher plants. FEBS Lett 499:87–91CrossRef

Hebant C (1977) The conducting tissues of bryophytes. J Cramer, Vaduz, Liechtenstein, p 157

Hohe A, Rensing SA, Mildner M, Lang D, Reski R (2002) Day length and temperature strongly influence sexual reproduction and expression of a novel MADS-box gene in the moss Physcomitrella patens. Plant Biol 4:595–602CrossRef

Holland N, Holland D, Helentjaris T, Dhugga KS, Xoconostle-Cazares B, Delmer DP (2000) A comparative analysis of the plant cellulose synthase (CesA) gene family. Plant Physiol 123:1313–1323CrossRef

Kamisugi Y, Schlink K, Rensing SA, Schween G, Stacklberg MV, Cuming AC, Reisk R, Cove D (2006) The mechanism of gene targeting in Physcomitrella patens: homologous recombination, concatenation and multiple integration. Nucleic Acids Res 34:6205–6214CrossRef

Kimura S, Laosinchai W, Itoh T, Cui XJ, Linder CR, Brown RM (1999) Immunogold labeling of rosette terminal cellulose-synthesizing complexes in the vascular plant Vigna angularis. Plant Cell 11:2075–2085CrossRef

Knight CD, Cove DJ, Cuming AC, Quatrano RS (2002) Moss gene technology. In: Gilmartin PM, Bowler C (eds) Molecular plant biology, vol 2. Oxford University Press, Oxford, pp 285–301

Kurek I, Kawagoe Y, Jacob-Wilk D, Doblin M, Delmer D (2002) Dimerization of cotton fiber cellulose synthase catalytic subunits occurs via oxidation of the zinc-binding domains. Proc Natl Acad Sci USA 99:11109–11114CrossRef

Muller SC, Brown RM Jr (1980) Evidence for an intramembranous component associated with a cellulose microfibril synthesizing complex in higher plants. J Cell Biol 84:315–316CrossRef

Nishiyama T, Hiwatashi Y, Sakakibara K, Kato M, Hasebe M (2000) Tagged mutagenesis and gene-trap in the moss, Physcomitrella patens by shuttle mutagenesis. DNA Res 7:1–9CrossRef

Pear JR, Kawagoe Y, Schreckengost WE, Delmer DP, Stalker DM (1996) Higher plants contain homologs of the bacterial celA genes encoding the catalytic sub-unit of cellulose synthase. Proc Natl Acad Sci USA 93:12637–12642CrossRef

Persson S, Paredez A, Carroll A, Palsdottir H, Doblin M, Poindexter P, Khitrov N, Auer M, Somerville CR (2007) Genetic evidence for three unique components in primary cell-wall cellulose synthase complexes in Arabidopsis. Proc Natl Acad Sci USA 104:15566–15571CrossRef

Rensing SA, Ick J, Fawcett JA, Lang D, Zimmer A, Van de Peer Y, Reski R (2007) An ancient genome duplication contributed to the abundance of metabolic genes in the moss Physcomitrella patens. BMC Evol Biol 7:130CrossRef

Richmond T (2000) Higher plant cellulose synthases. Genome Biol 4:30011–30016

Richmond TA, Somerville CR (2000) The cellulose synthase superfamily. Plant Physiol 124:495–498CrossRef

Roberts A, Bushoven JT (2007) The cellulose synthase (CesA) gene superfamily of the moss Physcomitrella patens. Plant Mol Biol 63:207–219CrossRef

Roberts AW, Roberts E (2004) Cellulose synthase (CesA) genes in algae and seedless plants. Cellulose 11:419–435CrossRef

Saxena IM, Lin FC, Brown RM Jr (1990) Cloning and sequencing of the cellulose synthase catalytic sub-unit gene of Acetobacter xylium. Plant Mol Biol 15:673–683CrossRef

Saxena IM, Brown RM Jr, Dandekar T (2001) Structure-function characterization of cellulose synthase: relationship to other glycosyltransferases. Phytochemistry 57:1135–1148CrossRef

Schaefer DG (2002) A new moss genetics: targeted mutagenesis in Physcomitrella patens. Annu Rev Plant Biol 53:477–501CrossRef

Schaefer DG, Zryd JP (1997) Efficient gene targeting in the moss Physcomitrella patens. Plant J 11:1195–1206CrossRef

Schaefer DG, Zryd JP (2001) The moss Physcomitrella patens, now and then. Plant Physiol 127:1430–1438CrossRef

Schaefer D, Zryd JP, Knight C, Cove DJ (1991) Stable transformation of the moss Physcomitrella patens. Mol Gen Genet 226:418–424

Smith GM (1955) Cryptogamic botany, vol II. Bryophytes and pteridophytes, 2nd edn. McGraw-Hill, New York

Somerville C (2006) Cellulose synthesis in higher plants. Annu Rev Cell Dev Biol 22:53–78CrossRef

Tanaka K, Murata K, Yamazaki M, Onosato K, Miyao A, Hirochika H (2003) Three distinct rice cellulose synthase catalytic subunit genes required for cellulose synthesis in the secondary wall. Plant Physiol 133:73–83CrossRef

Taylor NG (2008) Cellulose biosynthesis and deposition in higher plants. New Phyto 178:239–252CrossRef

Taylor NG, Laurie S, Turner SR (2000) Multiple cellulose synthase catalytic subunits are required for cellulose synthesis in Arabidopsis. Plant Cell 12:2529–2540CrossRef

Taylor NG, Howells RM, Huttly AK, Vickers K, Turner SR (2003) Interactions among three distinct CesA proteins essential for cellulose synthesis. Proc Natl Acad Sci USA 100:1450–1455CrossRef

Tseko I (1999) The sites of cellulose synthesis in algae: diversity and evolution of cellulose-synthesizing enzyme complexes. J Phycol 35:635–655CrossRef

Tusnády GE, Simon I (1998) Principles governing amino acid composition of integral membrane proteins: applications to topology prediction. J Mol Biol 283:489–506CrossRef

Tusnády GE, Simon I (2001) The HMMTOP transmembrane topology prediction server. Bioinformatics 17:849–850CrossRef

Wong HC, Fear AL, Calhoon RD, Eichinger GH, Mayer R, Amikam D, Benziman M, Gelfand DH, Meade JH, Emerick AW et al (1990) Genetic organization of the cellulose synthase operon in Acetobacter xylinum. Proc Natl Acad Sci USA 87:8130–8134CrossRef

Zhang JZ (2003) Evolution by gene duplication: an update. Trends Ecol Evol 18:292–298CrossRef

Title: Isolation and characterization of the cellulose synthase genes PpCesA6 and PpCesA7 in Physcomitrella patens
Authors: Hua Zhang Wise
Inder M. Saxena
R. Malcolm Brown Jr.
Publication date: 01-04-2011
Publisher: Springer Netherlands
Published in: Cellulose / Issue 2/2011
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-010-9479-6

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