Introduction
Materials and methods
Materials
Grafting of membranes
Contact angle
Study of swelling: pH-response
Mechanical tests
Load and release of vancomycin
Antimicrobial tests
Instrumental
Vacuum drying
Contact angle
Fourier transform infrared attenuated total reflection (FTIR-ATR)
Thermogravimetric analysis (TGA)
13C-CPMAS NMR
Scanning XPS
Mechanical test
SEM
Ultraviolet–visible (UV–vis)
Results and discussion
Synthesis and characterization of MS-g-4VP membranes
Water contact angle and pH responsiveness
FTIR-ATR study
Thermal analysis
Sample | Decomposition temperature (°C) |
---|---|
MS-g-4VP (31.0%) | 354, 434.1 |
MS-g-4VP (25.7%) | 264.2, 347, 388.1, 435.8 |
MS-g-4VP (13.2%) | 260.8, 336, 386.6, 436.8 |
MS | 252.4, 334, 412.2, 445 |
P4VP | 388 |
C-CPMAS NMR and XPS analysis
Atomic composition (%)* | |||
---|---|---|---|
C | N | O | |
MS outside part | 86.9 | 0.7 | 12.7 |
MS inside part | 80.9 | 1.9 | 17.2 |
MS-g-4VP (31.0%) outside part | 83.0 | 4.7 | 12.2 |
MS-g-4VP (31.0%) inside part | 76.7 | 8.1 | 15.2 |
Mechanical properties
Sample | Young’s modulus (MPa) | Tensile break (MPa) | Tenacity (MPa) | Elongation break (%) | Maximum load (N) |
---|---|---|---|---|---|
MS | 45.25 ± 2.26 | 4.8 ± 0.24 | 0.722 ± 0.036 | 23.2 ± 1.16 | 3 ± 0.15 |
MS-g-4VP (13.2%) | 76.58 + 3.82 | 7.5 ± 0.37 | 0.819 ± 0.040 | 19.3 ± 0.96 | 3 ± 0.18 |
MS-g-4VP (31.0%) | 74.90 ± 3.74 | 5.7 ± 0.28 | 0.557 ± 0.027 | 15.7 ± 0.78 | 3 ± 0.12 |
Scanning electron microscopy (SEM) study
Loading and releasing of vancomycin
Grafting degree (wt%) | cumulative vancomycin releasing at 7 h (μg%) | |
---|---|---|
pH = 5 | 0 | 23.50 |
13.2 | 28.95 | |
31.0 | 24.33 | |
pH = 7 | 0 | 85.13 |
13.2 | 81.62 | |
31.0 | 74.33 | |
pH = 9 | 0 | 89.39 |
13.2 | 55.74 | |
31.0 | 68.75 |
Graft (wt%) | Zero-order | First-Order | Higuchi | Korsmeyer-Peppas | |||
---|---|---|---|---|---|---|---|
R2 | R2 | R2 | Kha | R2 | nb | ||
pH 5 | 0 | 0.9160 | 0.9169 | 0.7882 | 1.430 | 0.9105 | 0.942 |
13.2 | 0.9475 | 0.9480 | 0.7721 | 1.887 | 0.9443 | 1.045 | |
31.0 | 0.9270 | 0.9258 | 0.7100 | 1.885 | 0.9443 | 1.045 | |
pH 7 | 0 | 0.3246 | 0.3424 | 0.8294 | 4.339 | 1 | 0.072 |
13.2 | 0.6265 | 0.6553 | 0.9730 | 3.845 | 0.9927 | 0.342 | |
31.0 | 0.7001 | 0.7152 | 0.9877 | 2.285 | 0.9967 | 0.389 | |
pH 9 | 0 | 0.3795 | 0.3985 | 0.6234 | 2.985 | 0.8684 | 0.733 |
13.2 | 0.7348 | 0.7560 | 0.8825 | 3.797 | 0.8861 | 0.588 | |
31.0 | 0.8299 | 0.8405 | 0.8197 | 3.241 | 0.8674 | 0.733 |