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2020 | OriginalPaper | Chapter

13. Microbial Identification and Extracellular Polymeric Substances Characterization of Aerobic Granules Developed in Treating Rubber Processing Wastewater

Authors : Aznah Nor Anuar, Mohd Hakim Ab Halim, Noor Hasyimah Rosman, Inawati Othman, Hasnida Harun, Hazlami Fikri Basri, Zaini Ujang, Mark van Loosdrecht

Published in: Valorisation of Agro-industrial Residues – Volume I: Biological Approaches

Publisher: Springer International Publishing

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Abstract

The goal of this study was to investigate the species of the abundance microbial in seed sludge and aerobic granular sludge. Experiments were carried out in a sequencing batch reactor with a working volume of 1.6 L. During the start-up period, the reactor was inoculated with 800 mL of sludge from a municipal sewage treatment plant plus 800 mL of rubber processing wastewater. Further investigation by Illumina high-throughput sequencing was performed to analyze the microbial diversity and phylogenetic structures during the granulation of seed sludge to aerobic granules. A diversity of microorganisms was identified from the seed sludge and aerobic granular sludge. Seed sludge consisted of 96.4% bacteria, 1.7% eukaryote, 1.2% archaea, and 0.7% viruses. Aerobic granular sludge consisted of 97.8% bacteria, 1.0% eukaryote, 0.8% archaea, and 0.4% viruses. As the granulation process succeeded in SBR, distinct differences of the microbial community in the seed sludge and aerobic granular sludge were observed, which suggested that there was high microbial selection pressure during granulation in the system. The most abundance species in seed sludge was Dechloromonas aromatica, while Pseudomonas fluorescens was the most abundance species in aerobic granular sludge.

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Title: Microbial Identification and Extracellular Polymeric Substances Characterization of Aerobic Granules Developed in Treating Rubber Processing Wastewater
Authors: Aznah Nor Anuar
Mohd Hakim Ab Halim
Noor Hasyimah Rosman
Inawati Othman
Hasnida Harun
Hazlami Fikri Basri
Zaini Ujang
Mark van Loosdrecht
Publisher: Springer International Publishing
Book: Valorisation of Agro-industrial Residues – Volume I: Biological Approaches
Print ISBN: 978-3-030-39136-2

Electronic ISBN: 978-3-030-39137-9

Copyright Year: 2020
DOI: https://doi.org/10.1007/978-3-030-39137-9_13