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Medical & Biological Engineering & Computing

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01-10-2016 | Special Issue - Original Article

Microscopic histological characteristics of soft tissue sarcomas: analysis of tissue features and electrical resistance

Authors: A. L. Tosi, L. G. Campana, F. Dughiero, M. Forzan, M. Rastrelli, E. Sieni, C. R. Rossi

Published in: Medical & Biological Engineering & Computing | Issue 7/2017

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Abstract

Tissue electrical conductivity is correlated with tissue characteristics. In this work, some soft tissue sarcomas (STS) excised from patients have been evaluated in terms of histological characteristics (cell size and density) and electrical resistance. The electrical resistance has been measured using the ex vivo study on soft tissue tumors electrical characteristics (ESTTE) protocol proposed by the authors in order to study electrical resistance of surgical samples excised by patients in a fixed measurement setup. The measurement setup includes a voltage pulse generator (700 V, 100 µs long at 5 kHz, period 200 µs) and an electrode with 7 needles, 20 mm-long, with the same distance arranged in a fixed hexagonal geometry. In the ESTTE protocol, the same voltage pulse sequence is applied to each different tumor mass and the corresponding resistance has been evaluated from voltage and current recorded by the equipment. For each tumor mass, a histological sample of the volume treated by means of voltage pulses has been taken for histological analysis. Each mass has been studied in order to identify the sarcoma type. For each histological sample, an image at 20× or 40× of magnification was acquired. In this work, the electrical resistance measured for each tumor has been correlated with tissue characteristics like the type, size and density of cells. This work presents a preliminary study to explore possible correlations between tissue characteristics and electrical resistance of STS. These results can be helpful to adjust the pulse voltage intensity in order to improve the electrochemotherapy efficacy on some histotype of STS.

previous article Mathematical model of tumor volume dynamics in mice treated with electrochemotherapy

next article Multi-parametric study of temperature and thermal damage of tumor exposed to high-frequency nanosecond-pulsed electric fields based on finite element simulation

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ECT

Eletrochemiotherapy

STS

Soft tissue sarcomas

ESTTE

Ex vivo study on soft tissue tumors electrical characteristics

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Title: Microscopic histological characteristics of soft tissue sarcomas: analysis of tissue features and electrical resistance
Authors: A. L. Tosi
L. G. Campana
F. Dughiero
M. Forzan
M. Rastrelli
E. Sieni
C. R. Rossi
Publication date: 01-10-2016
Publisher: Springer Berlin Heidelberg
Published in: Medical & Biological Engineering & Computing / Issue 7/2017
Print ISSN: 0140-0118
Electronic ISSN: 1741-0444
DOI: https://doi.org/10.1007/s11517-016-1573-y

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