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Colloid and Polymer Science

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01-12-2013 | Original Contribution

Monitoring mesoglobules formation in PNIPAm solutions using Nile Red solvatochromism

Authors: Clémentine Locatelli-Champagne, Michel Cloitre

Published in: Colloid and Polymer Science | Issue 12/2013

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Abstract

Nile Red solvatochromism is used to monitor phase separation in concentrated poly(N-isopropyl acrylamide) (PNIPAm) aqueous solutions. Below the lower critical solution temperature (LCST), Nile Red molecules are in a polar environment and thus exhibit negligible fluorescence. Above the LCST, the aggregation of the PNIPAm chains into hydrophobic mesoglobules provides a nonpolar environment, causing a strong increase of fluorescence. The spectra show two emission bands, which can be related to the partitioning of Nile Red molecules between the core and the surface of the mesoglobules. More generally, the technique appears as a new and promising tool to probe microheterogeneities in polymer solutions or mixtures.

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Note that in our experiments, the polymer solutions are quenched to the unstable region of the phase diagram directly from the soluble region. The approximation may not be correct for other thermal histories. For instance, if the solution was subjected to a thermal ramp, dye molecules at the surface would have to migrate to the interior of the mesoglobules as their surface area reduces. It is more likely that they would remain on the surface thereby increasing the superficial density.

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Title: Monitoring mesoglobules formation in PNIPAm solutions using Nile Red solvatochromism
Authors: Clémentine Locatelli-Champagne
Michel Cloitre
Publication date: 01-12-2013
Publisher: Springer Berlin Heidelberg
Published in: Colloid and Polymer Science / Issue 12/2013
Print ISSN: 0303-402X
Electronic ISSN: 1435-1536
DOI: https://doi.org/10.1007/s00396-013-3035-5

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