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Journal of Nanoparticle Research

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01-10-2018 | Research Paper

Morphomechanical and organelle perturbation induced by silver nanoparticle exposure

Authors: Valeria De Matteis, Mariafrancesca Cascione, Chiara Cristina Toma, Stefano Leporatti

Published in: Journal of Nanoparticle Research | Issue 10/2018

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Abstract

The unique physico-chemical properties of silver nanoparticles (AgNPs) make them a powerful tool in many fields, ranging from cosmetics, biomedicals, household products, and wound dressing. Several evidences suggest the strong toxicity of AgNPs both in vitro and in vivo, but few data are available to full understanding of their adverse effects on cellular components and cytoskeleton. In this work, we assessed the toxicity of citrate-capped AgNPs on cortical actin and organelles, namely mitochondria and lysosomes, on epithelial breast cancer cells (MCF-7). The impact of AgNPs on cells was firstly evaluated in term of viability, oxidative stress, mitochondria membrane potential alteration, and apoptosis activation. Afterwards, we carefully estimated the qualitative and quantitative morphological alterations of cortical F-actin and organelles by confocal microscopy and specific software tools, coupled with a biomechanical analysis by atomic force microscopy (AFM). This multidisciplinary approach, which combines the standard biological assays with systematic morphometric and biomechanical analysis on cells, permits to understand at different levels the intracellular response elicited by AgNPs in order to provide new scenarios in toxicity assessment.

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Title: Morphomechanical and organelle perturbation induced by silver nanoparticle exposure
Authors: Valeria De Matteis
Mariafrancesca Cascione
Chiara Cristina Toma
Stefano Leporatti
Publication date: 01-10-2018
Publisher: Springer Netherlands
Published in: Journal of Nanoparticle Research / Issue 10/2018
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI: https://doi.org/10.1007/s11051-018-4383-3

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