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26-02-2021 | Original Research

Novel chitosan/gelatin/oxidized cellulose sponges as absorbable hemostatic agents

Authors: Jalal Ranjbar, Mojtaba Koosha, Hong Chi, Amir Ghasemi, Fatemeh Zare, Mohammad Amin Abdollahifar, Mohammad Darvishi, Tianduo Li

Published in: Cellulose | Issue 6/2021

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Abstract

Chitosan/gelatin (CG) composite sponges containing oxidized cellulose fibers (OF) crosslinked with tannic acid were fabricated by freeze-drying as hemostatic agents. The cellulose fibers were oxidized by 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO). Fourier transform infrared spectroscopy proved the formation of carboxyl groups on cellulose fibers. Addition of OF increased the swelling ratio of CG sponges. The amount of bleeding was measured after 10 s on hepatic trauma in a rat model. To assess the in vivo bio-absorbability of the samples, the sponges were implanted in the liver for 21 days and the volume of the repaired tissue was measured from histopathological analysis. The biocomposite sponge fabricated by addition of 30% OF suspension into CG solution showed the lowest amount of bleeding and the highest bio-absorbability after 21 days. The findings of this study indicate that the addition of OF into CG increased the clotting capability as well as the bio-absorbability of the biocomposite sponges.

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Abraham LC, Zuena E, Perez-Ramirez B, Kaplan DL (2008) Guide to collagen characterization for biomaterial studies. J Biomed Mater Res Part B Appl Biomater 87B:264–285. https://doi.org/10.1002/jbm.b.31078CrossRef

Agrawal P, Soni S, Mittal G, Bhatnagar A (2014) Role of polymeric biomaterials as wound healing agents. Int J Low Extrem Wounds 13:180–190CrossRef

Alam HB, Burris D, DaCorta JA, Rhee P (2005) Hemorrhage control in the battlefield: role of new hemostatic. Agents Military Med 170:63–69. https://doi.org/10.7205/milmed.170.1.63CrossRef

Aydemir Sezer U, Sahin İ, Aru B, Olmez H, Yanıkkaya Demirel G, Sezer S (2019) Cytotoxicity, bactericidal and hemostatic evaluation of oxidized cellulose microparticles: structure and oxidation degree approach. Carbohydr Polym 219:87–94. https://doi.org/10.1016/j.carbpol.2019.05.005CrossRefPubMed

Bagher Z et al (2019) Wound healing with alginate/chitosan hydrogel containing hesperidin in rat model. J Drug Deliv Sci Technol. https://doi.org/10.1016/j.jddst.2019.101379CrossRef

Barhoum A, Pal K, Rahier H, Uludag H, Kim IS, Bechelany M (2019) Nanofibers as new-generation materials: from spinning and nano-spinning fabrication techniques to emerging applications. Appl Mater Today 17:1–35. https://doi.org/10.1016/j.apmt.2019.06.015CrossRef

Chen XY, Low HR, Loi XY, Merel L, Mohd Cairul Iqbal MA (2019) Fabrication and evaluation of bacterial nanocellulose/poly (acrylic acid)/graphene oxide composite hydrogel: characterizations and biocompatibility studies for wound dressing. J Biomed Mater Res Part B 107B:2140–2151. https://doi.org/10.1002/jbm.b.34309CrossRef

Cheng F, Liu C, Wei X, Yan T, Li H, He J, Huang Y (2017) Preparation and characterization of 2, 2, 6, 6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized cellulose nanocrystal/alginate biodegradable composite dressing for hemostasis applications. ACS Sustain Chem Eng 5:3819–3828CrossRef

Curvello R, Raghuwanshi VS, Garnier G (2019) Engineering nanocellulose hydrogels for biomedical applications. Adv Colloid Interface Sci 267:47–61CrossRef

Fathollahipour S, Mehrizi A, Ghaee A, Koosha M (2015) Electrospinning of PVA/chitosan nanocomposite nanofibers containing gelatin nanoparticles as a dual drug delivery system. J Biomed Mater Res Part A. https://doi.org/10.1002/jbm.a.35529CrossRef

Fujisawa S, Okita Y, Fukuzumi H, Saito T, Isogai A (2011) Preparation and characterization of TEMPO-oxidized cellulose nanofibril films with free carboxyl groups. Carbohydr Polym 84:579–583. https://doi.org/10.1016/j.carbpol.2010.12.029CrossRef

Ghafari R, Jonoobi M, Amirabad LM, Oksman K, Taheri AR (2019) Fabrication and characterization of novel bilayer scaffold from nanocellulose based aerogel for skin tissue engineering applications. Int J Biol Macromol 136:796–803. https://doi.org/10.1016/j.ijbiomac.2019.06.104CrossRefPubMed

Gu BK, Park SJ, Kim MS, Lee YJ, Kim J-I, Kim C-H (2016) Gelatin blending and sonication of chitosan nanofiber mats produce synergistic effects on hemostatic functions. Int J Biol Macromol 82:89–96. https://doi.org/10.1016/j.ijbiomac.2015.10.009CrossRefPubMed

Halim ALA, Kamari A, Phillip E (2018) Chitosan, gelatin and methylcellulose films incorporated with tannic acid for food packaging International. J Biol Macromol 120:1119–1126. https://doi.org/10.1016/j.ijbiomac.2018.08.169CrossRef

Harkins AL, Duri S, Kloth LC, Tran CD (2014) Chitosan–cellulose composite for wound dressing material. Part 2. Antimicrobial activity, blood absorption ability, and biocompatibility. J Biomed Mater Res Part B Appl Biomater 102:1199–1206CrossRef

Hu Z, Zhang D-Y, Lu S-T, Li P-W, Li S-D (2018) Chitosan-based composite materials for prospective hemostatic applications. Mar Drugs 16(8):273. https://doi.org/10.3390/md16080273CrossRefPubMedCentral

Huang H et al (2019) Degradable and bioadhesive alginate-based composites: an effective hemostatic agent. ACS Biomater Sci Eng 5:5498–5505. https://doi.org/10.1021/acsbiomaterials.9b01120CrossRefPubMed

Isogai A, Saito T, Fukuzumi H (2011) TEMPO-oxidized cellulose nanofibers. Nanoscale 3:71–85. https://doi.org/10.1039/C0NR00583ECrossRefPubMed

Jiang Q, Xu J, Li T, Qiao C, Li Y (2014) Synthesis and antibacterial activities of quaternary ammonium salt of gelatin. J Macromol Sci Part B 53:133–141. https://doi.org/10.1080/00222348.2013.808518CrossRef

Kalantari K, Afifi AM, Jahangirian H, Webster TJ (2019) Biomedical applications of chitosan electrospun nanofibers as a green polymer—review. Carbohydr Polym 207:588–600. https://doi.org/10.1016/j.carbpol.2018.12.011CrossRefPubMed

Kauvar D, Lefering R, Wade C (2006) Impact of hemorrhage on trauma outcome: an overview of epidemiology, clinical presentations, and therapeutic considerations. J Trauma Injury Infect Criti Care 60:S3–S11CrossRef

Kim GH, Im JN, Kim TH, Lee GD, Youk JH, Doh SJ (2018) Preparation and characterization of calcium carboxymethyl cellulose/chitosan blend nonwovens for hemostatic agents. Text Res J 88:1902–1911CrossRef

Kono H, Fujita S (2012) Biodegradable superabsorbent hydrogels derived from cellulose by esterification crosslinking with 1, 2, 3, 4-butanetetracarboxylic dianhydride. Carbohydr Polym 87:2582–2588CrossRef

Koosha M, Hamedi S (2019) Intelligent chitosan/PVA nanocomposite films containing black carrot anthocyanin and bentonite nanoclays with improved mechanical, thermal and antibacterial properties. Prog Org Coat 127:338–347. https://doi.org/10.1016/j.porgcoat.2018.11.028CrossRef

Koosha M, Mirzadeh H (2015) Electrospinning, mechanical properties, and cell behavior study of chitosan/PVA nanofibers. J Biomed Mater Res Part A 103:3081–3093. https://doi.org/10.1002/jbm.a.35443CrossRef

Lan G et al (2015) Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent. Colloids Surf B 136:1026–1034. https://doi.org/10.1016/j.colsurfb.2015.10.039CrossRef

Li Y, Qiao C, Shi L, Jiang Q, Li T (2014) Viscosity of collagen solutions: influence of concentration, temperature, adsorption, and role of intermolecular interactions. J Macromol Sci Part B 53:893–901. https://doi.org/10.1080/00222348.2013.852059CrossRef

Li L et al (2019) Preparation and the hemostatic property study of porous gelatin microspheres both in vitro and in vivo. Colloids Surf B. https://doi.org/10.1016/j.colsurfb.2019.110641CrossRef

Lin N, Bruzzese C, Dufresne A (2012) TEMPO-oxidized nanocellulose participating as crosslinking aid for alginate-based sponges. ACS Appl Mater Interfaces 4:4948–4959. https://doi.org/10.1021/am301325rCrossRefPubMed

LogithKumar R, KeshavNarayan A, Dhivya S, Chawla A, Saravanan S, Selvamurugan N (2016) A review of chitosan and its derivatives in bone tissue engineering. Carbohydr Polym 151:172–188. https://doi.org/10.1016/j.carbpol.2016.05.049CrossRefPubMed

Mohammadkazemi F, Khademi Barangenani R, Koosha M (2019a) Development of organic–inorganic oxidized bacterial cellulose nanobiocomposites: ternary complexes. Cellulose 26:6009–6022. https://doi.org/10.1007/s10570-019-02514-wCrossRef

Mohammadkazemi F, Khademibarangenani R, Koosha M (2019b) The effect of oxidation time and concentration on physicochemical, structural, and thermal properties of bacterial nano-cellulose. Polym Sci Ser A 61:265–273. https://doi.org/10.1134/S0965545X19030088CrossRef

Morrissey JH et al (2010) Protein–phospholipid interactions in blood clotting. Thromb Res 125:S23–S25. https://doi.org/10.1016/j.thromres.2010.01.027

Naimer SA (2014) A review of methods to control bleeding from life-threatening traumatic wounds. Health 6:479CrossRef

Nguyen VC, Nguyen VB, Hsieh M-F (2013) Curcumin-loaded chitosan/gelatin composite sponge for wound healing application. Int J Polym Sci 2013:106570. https://doi.org/10.1155/2013/106570CrossRef

Osorio M, Fernández-Morales P, Gañán P, Zuluaga R, Kerguelen H, Ortiz I, Castro C (2019) Development of novel three‐dimensional scaffolds based on bacterial nanocellulose for tissue engineering and regenerative medicine: effect of processing methods, pore size, and surface area. J Biomed Mater Res Part A 107:348–359CrossRef

Rubentheren V, Ward TA, Chee CY, Tang CK (2015) Processing and analysis of chitosan nanocomposites reinforced with chitin whiskers and tannic acid as a crosslinker. Carbohydr Polym 115:379–387CrossRef

Shefa AA, Taz M, Hossain M, Kim YS, Lee SY, Lee B-T (2019) Investigation of efficiency of a novel, zinc oxide loaded TEMPO-oxidized cellulose nanofiber based hemostat for topical bleeding. Int J Biol Macromol 126:786–795. https://doi.org/10.1016/j.ijbiomac.2018.12.079CrossRefPubMed

Sukul M, Ventura RD, Bae SH, Choi HJ, Lee SY, Lee BT (2017) Plant-derived oxidized nanofibrillar cellulose-chitosan composite as an absorbable hemostat. Mater Lett 197:150–155. https://doi.org/10.1016/j.matlet.2017.03.102CrossRef

Taheri P, Jahanmardi R, Koosha M, Abdi S (2020) Physical, mechanical and wound healing properties of chitosan/gelatin blend films containing tannic acid and/or bacterial nanocellulose. Int J Biol Macromol 154:421–432CrossRef

Tang A et al (2019) Nanocellulose/PEGDA aerogel scaffolds with tunable modulus prepared by stereolithography for three-dimensional cell culture. J Biomater Sci Polym Ed 30:1–18CrossRef

Wei L, Sun R, Liu C, Xiong J, Qin X (2019) Mass production of nanofibers from needleless electrospinning by a novel annular spinneret. Mater Des 179:107885. https://doi.org/10.1016/j.matdes.2019.107885CrossRef

Wu Y, He J, Cheng W, Gu H, Guo Z, Gao S, Huang Y (2012) Oxidized regenerated cellulose-based hemostat with microscopically gradient structure. Carbohydr Polym 88:1023–1032. https://doi.org/10.1016/j.carbpol.2012.01.058CrossRef

Xu W et al (2019) On low-concentration inks formulated by nanocellulose assisted with gelatin methacrylate (gelma) for 3D printing toward wound healing application. ACS Appl Mater Interfaces 11:8838–8848CrossRef

Yuan H, Chen L, Hong FF Healing (2020) A biodegradable antibacterial nanocomposite based on oxidized bacterial nanocellulose for rapid hemostasis and wound. ACS Appl Mater Interfaces 12:3382–3392. https://doi.org/10.1021/acsami.9b17732CrossRefPubMed

Zhang M, Wu Y, Zhang Q, Xia Y, Li T (2011) Synthesis and characterization of gelatin-polydimethylsiloxane graft copolymers. J Appl Polym Sci 120:2130–2137. https://doi.org/10.1002/app.33391CrossRef

Zhang S, Li J, Chen S, Zhang X, Ma J, He J (2019) Oxidized cellulose-based hemostatic materials Carbohydr Polym. https://doi.org/10.1016/j.carbpol.2019.115585

Title: Novel chitosan/gelatin/oxidized cellulose sponges as absorbable hemostatic agents
Authors: Jalal Ranjbar
Mojtaba Koosha
Hong Chi
Amir Ghasemi
Fatemeh Zare
Mohammad Amin Abdollahifar
Mohammad Darvishi
Tianduo Li
Publication date: 26-02-2021
Publisher: Springer Netherlands
Published in: Cellulose / Issue 6/2021
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-021-03699-9

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