Top

Published in:

2011 | OriginalPaper | Chapter

21. Plant Plasma Membrane Proteomics: Challenges and Possibilities

Authors : Anders Laurell Blom Møller, Katja Witzel, Annelies Vertommen, Vibeke Barkholt, Birte Svensson, Sebastien Carpentier, Hans-Peter Mock, Christine Finnie

Published in: Sample Preparation in Biological Mass Spectrometry

Publisher: Springer Netherlands

Activate our intelligent search to find suitable subject content or patents.

search-config

AI-assisted search

Off

Abstract

Plasma membrane proteins are challenging to study due to their hydrophobicity and low abundance. Extraction of plasma membranes from plants is complicated by the rigid plant cell wall. The most commonly used method to enrich for plant plasma membranes is the aqueous-polymer two-phase system. Many gel-based separation techniques have been used in plasma membrane proteomics studies including one-dimensional SDS-PAGE, classical two-dimensional electrophoresis based on isoelectric focussing and SDS-PAGE, double SDS-PAGE, Blue Native-PAGE and 16-BAC-PAGE. Some of the difficulties associated with gel-based separation of hydrophobic proteins can be bypassed by the use of gel-free approaches which involve in-solution separation of proteins or liquid chromatography-based separation of peptides. Mass spectrometry is subsequently used for identification of proteins. An overview is presented of the most commonly used methods for analysis of plant plasma membrane proteomes, their strengths and weaknesses.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

über 102.000 Bücher
über 537 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Finance + Banking
Management + Führung
Marketing + Vertrieb
Maschinenbau + Werkstoffe
Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

über 67.000 Bücher
über 390 Zeitschriften

aus folgenden Fachgebieten:

Automobil + Motoren
Bauwesen + Immobilien
Business IT + Informatik
Elektrotechnik + Elektronik
Energie + Nachhaltigkeit
Maschinenbau + Werkstoffe

Jetzt Wissensvorsprung sichern!

inform now

previous chapter Efficient Strategies for Analysis of Low Abundance Proteins in Plant Proteomics

next chapter Affinity and Chemical Enrichment for Mass Spectrometry-Based Proteomics Analyses

Akiyama, Y., and Ito, K. (1985). The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins. EMBO J 4, 3351–3356.

Albertsson, P.Å., and Tjerneld, F. (1994). Phase diagrams. Methods Enzymol 228, 3–13.

Alexandersson, E., Saalbach, G., Larsson, C., and Kjellbom, P. (2004). Arabidopsis plasma membrane proteomics identifies components of transport, signal transduction and membrane trafficking. Plant Cell Physiol 45, 1543–1556.

Bardy, N., Carrasco, A., Galaud, J.P., Pont-Lezica, R., and Canut, H. (1998). Free-flow electrophoresis for fractionation of Arabidopsis thaliana membranes. Electrophoresis 19, 1145–1153.

Barkla, B.J., Vera-Estrella, R., and Pantoja, O. (2007). Enhanced separation of membranes during free flow zonal electrophoresis in plants. Anal Chem 79, 5181–5187.

Beyreuther, K., Bieseler, B., Ehring, R., Griesser, H.W., Mieschendahl, M., Müller-Hill, B., and Triesch, I. (1980). Investigation of structure and function of lactose permease of Escherichia coli. Biochem Soc Trans 8, 675–676.

Blonder, J., Terunuma, A., Conrads, T.P., Chan, K.C., Yee, C., Lucas, D.A., Schaefer, C.F., Yu, L.R., Issaq, H.J., Veenstra, T.D., and Vogel, J.C. (2004). A proteomic characterization of the plasma membrane of human epidermis by high-throughput mass spectrometry. J Invest Dermatol 123, 691–699.

Borner, G.H., Sherrier, D.J., Weimar, T., Michaelson, L.V., Hawkins, N.D., Macaskill, A., Napier, J.A., Beale, M.H., Lilley, K.S., and Dupree, P. (2005). Analysis of detergent-resistant membranes in Arabidopsis. Evidence for plasma membrane lipid rafts. Plant Physiol 137, 104–116.

Braun, R.J., Kinkl, N., Beer, M., and Ueffing, M. (2007). Two-dimensional electrophoresis of membrane proteins. Anal Bioanal Chem 389, 1033–1045.

Brooks, D.E., and Norris-Jones, R. (1994). Preparation and analysis of 2-phase systems. Methods Enzymol 228, 14–27.

Burré, J., Beckhaus, T., Schägger, H., Corvey, C., Hofmann, S., Karas, M., Zimmermann, H., and Volknandt, W. (2006). Analysis of the synaptic vesicle proteome using three gel-based protein separation techniques. Proteomics 6, 6250–6262.

Canut, H., Bauer, J., and Weber, G. (1999). Separation of plant membranes by electromigration techniques. J Chromatogr B Biomed Sci Appl 722, 121–139.

Carletti, P., Masi, A., Spolaore, B., Polverino De Laureto, P., De Zorzi, M., Turetta, L., Ferretti, M., and Nardi, S. (2008). Protein expression changes in maize roots in response to humic substances. J Chem Ecol 34, 804–818.

Carpentier, S.C., Panis, B., Vertommen, A., Swennen, R., Sergeant, K., Renaut, J., Laukens, K., Witters, E., Samyn, B., and Devreese, B. (2008). Proteome analysis of non-model plants: A challenging but powerful approach. Mass Spectrom Rev 27, 354–377.

Chen, F., Li, Q., and He, Z. (2007). Proteomic analysis of rice plasma membrane-associated proteins in response to Chitooligosaccharide elicitors. J Integr Plant Biol 49, 863−870.

Chen, F., Yuan, Y., Li, Q., and He, Z. (2007). Proteomic analysis of rice plasma membrane reveals proteins involved in early defense response to bacterial blight. Proteomics 7, 1529–1539.

Cheng, Y., Qi, Y., Zhu, Q., Chen, X., Wang, N., Zhao, X., Chen, H., Cui, X., Xu, L., and Zhang, W. (2009). New changes in the plasma-membrane-associated proteome of rice roots under salt stress. Proteomics 9, 3100–3114.

Coughenour, H.D., Spaulding, R.S., and Thompson, C.M. (2004). The synaptic vesicle proteome: A comparative study in membrane protein identification. Proteomics 4, 3141–3155.

Deswarte, C., Peyrebrune, S., Canut, H., Roustan, J.P., and Fallot, J. (1994). Purification of plasma-membrane vesicles from Vitis-vinifera cv Gamay suspension cells by free-flow electrophoresis. Vitis 33, 99–100.

Dunkley, T.P.J., Hester, S., Shadforth, I.P., Runions, J., Weimar, T., Hanton, S.L., Griffin, J.L., Bessant, C., Brandizzi, F., Hawes, C., Watson, R.B., Dupree, P., and Lilley, K.S. (2006). Mapping the Arabidopsis organelle proteome. Proc Natl Acad Sci USA 103, 6518–6523.

Egger, A., Morre, J., Sellden, G., and Sandelius, A.S. (1992). Isolation of plasma-membrane fractions from green wheat leaves by free-flow electrophoresis or 2-phase partition alone or in combination. Physiol Plant 84, 121–133.

Elschenbroich, S., Ignatchenko, V., Sharma, P., Schmitt-Ulms, G., Gramolini, A.O., and Kislinger, T. (2009). Peptide separations by on-line MudPIT compared to isoelectric focusing in an Off-Gel format: Application to a membrane-enriched fraction from C2C12 mouse skeletal muscle cells. J Proteome Res 8, 4860–4869.

Fujiwara, M., Hamada, S., Hiratsuka, M., Fukao, Y., Kawasaki, T., and Shimamoto, K. (2009). Proteome analysis of detergent-resistant membranes (DRMs) associated with OsRac1 mediated innate immunity in rice. Plant Cell Physiol 50, 1191–200.

Han, M.J., Herlyn, M., Fisher, A.B., and Speicher, D.W. (2008). Microscale solution IEF combined with 2-D DIGE substantially enhances analysis depth of complex proteomes such as mammalian cell and tissue extracts. Electrophoresis 29, 695–705.

Han, M.J., and Speicher, D.W. (2008). Microscale isoelectric focusing in solution: A method for comprehensive and quantitative proteome analysis using 1-D and 2-D DIGE combined with MicroSol IEF prefractionation. Methods Mol Biol 424, 241–256.

Hartinger, J., Stenius, K., Högemann, D., and Jahn, R. (1996). 16-BAC/SDS-PAGE: A two-dimensional gel electrophoresis system suitable for the separation of integral membrane proteins. Anal Biochem 240, 126–133.

Hashimoto, M., Toorchi, M., Matsushita, K., Iwasaki, Y., and Komatsu, S. (2009). Proteome analysis of rice root plasma membrane and detection of cold stress responsive proteins. Protein Pept Lett 16, 685–697.

Heinemeyer, J., Scheibe, B., Schmitz, U.K., and Braun, H.P. (2009). Blue native DIGE as a tool for comparative analyses of protein complexes. J Proteomics 72, 539–544.

Helling, S., Schmitt, E., Joppich, C., Schulenborg, T., Mullner, S., Felske-Muller, S., Wiebringhaus, T., Becker, G., Linsenmann, G., Sitek, B., Lutter, P., Meyer, H.E., and Marcus, K. (2006). 2-D differential membrane proteome analysis of scarce protein samples. Proteomics 6, 4506–4513.

Hynek, R., Svensson, B., Jensen, O.N., Barkholt, V., and Finnie, C. (2009). The plasma membrane proteome of germinating barley embryos. Proteomics 9, 3787–3794.

Hynek, R., Svensson, B., Jensen, O.N., Barkholt, V., and Finnie, C. (2006). Enrichment and identification of integral membrane proteins from barley aleurone layers by reversed-phase chromatography, SDS-PAGE, and LC-MS/MS. J Proteome Res 5, 3105–3113.

Johansson, F., Olbe, M., Sommarin, M., and Larsson, C. (1995). Brij 58, a polyoxyethylene acyl ether, creates membrane vesicles of uniform sidedness. A new tool to obtain inside-out (cytoplasmic side-out) plasma membrane vesicles. Plant J 7, 165–173.

Jung, C., Higgins, C.M., and Xu, Z. (2000). Measuring the quantity and activity of mitochondrial electron transport chain complexes in tissues of central nervous system using blue native polyacrylamide gel electrophoresis. Anal Biochem 286, 214–223.

Juszczuk, I.M., and Rychter, A.M. (2009). BN-PAGE analysis of the respiratory chain complexes in mitochondria of cucumber MSC16 mutant. Plant Physiol Biochem 47, 397–406.

Katz, A., Waridel, P., Shevchenko, A., and Pick, U. (2007). Salt-induced changes in the plasma membrane proteome of the halotolerant alga Dunaliella salina as revealed by blue native gel electrophoresis and nano-LC-MS/MS analysis. Mol Cell Proteomics 6, 1459–1472.

Kjell, J., Rasmusson, A.G., Larsson, H., and Widell, S. (2004). Protein complexes of the plant plasma membrane resolved by Blue Native PAG. Physiol Plant 121, 546–555.

Kline, K.G., and Wu, C.C. (2009). MudPIT analysis: Application to human heart tissue. Methods Mol Biol 528, 281–293.

Komatsu, S., Wada, T., Abaléa, Y., Nouri, M.Z., Nanjo, Y., Nakayama, N., Shimamura, S., Yamamoto, R., Nakamura, T., and Furukawa, K. (2009). Analysis of plasma membrane proteome in soybean and application to flooding stress response. J Proteome Res 8, 4487–4499.

Kramer, M.L. (2006). A new multiphasic buffer system for benzyldimethyl-n-hexadecylammonium chloride polyacrylamide gel electrophoresis of proteins providing efficient stacking. Electrophoresis 27, 347–356.

Lambert, J.P., Ethier, M., Smith, J.C., and Figeys, D. (2005). Proteomics: From gel based to gel free. Anal Chem 77(12):3771–3787.

Larsson, C., Widell, S., and Kjellbom, P. (1987). Preparation of high-purity plasma membrane. Methods Enzymol 148, 558–568

Lee, Y.C., Block, G., Chen, H., Folch-Puy, E., Foronjy, R., Jalili, R., Jendresen, C.B., Kimura, M., Kraft, E., Lindemose, S., Lu, J., McLain, T., Nutt, L., Ramon-Garcia, S., Smith, J., Spivak, A., Wang, M.L., Zanic, M., and Lin, S.H. (2008). One-step isolation of plasma membrane proteins using magnetic beads with immobilized concanavalin A. Protein Exp Purif 62, 223–229.

Lee, J., Garrett, W.M., and Cooper, B. (2007). Shotgun proteomic analysis of Arabidopsis thaliana leaves. J Sep Sci 30, 2225–2230.

Lefebvre, B., Furt, F., Hartmann, M.A., Michaelson, L.V., Carde, J.P., Sargueil-Boiron, F., Rossignol, M., Napier, J.A., Cullimore, J., Bessoule, J.J., and Mongrand, S. (2007). Characterization of lipid rafts from Medicago truncatula root plasma membranes: A proteomic study reveals the presence of a raft-associated redox system. Plant Physiol 144, 402–418.

Lu, B., McClatchy, D.B., Kim, J.Y., and Yates, J.R. III (2008). Strategies for shotgun identification of integral membrane proteins by tandem mass spectrometry. Proteomics 8, 3947–3955.

Luche, S., Santoni, V., and Rabilloud, T. (2003). Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 249–253.

Lüthje, S., Hopff, D., Schmitt, A., Meisrimler, C.N., and Menckhoff, L. (2009). Hunting for low abundant redox proteins in plant plasma membranes. J Proteomics 72, 475–483.

Macfarlane, D.E. (1983). Use of benzyldimethyl-n-hexadecylammonium chloride ("16-BAC"), a cationic detergent, in an acidic polyacrylamide gel electrophoresis system to detect base labile protein methylation in intact cells. Anal Biochem 132, 231–235.

Malakshah, S.N., Habibi Rezaei, M., Heidari, M., and Salekdeh, G.H. (2007). Proteomics reveals new salt responsive proteins associated with rice plasma membrane. Biosci Biotechnol Biochem 71, 2144–2154.

Malmstrom, J., Lee, H., Nesvizhskii, A.I., Shteynberg, D., Mohanty, S., Brunner, E., Ye, M.L., Weber, G., Eckerskorn, C., and Aebersold, R. (2006). Optimized peptide separation and identification for mass spectrometry based proteomics via free-flow electrophoresis. J Proteome Res 5, 2241–2249.

Marmagne, A., Ferro, M., Meinnel, T., Bruley, C., Kuhn, L., Garin, J., Barbier-Brygoo, H., and Ephritikhine, G. (2007). A high content in lipid-modified peripheral proteins and integral eceptor kinases features in the arabidopsis plasma membrane proteome. Mol Cell Proteomics 6, 1980–1996.

Meyer, B., Wittig, I., Trifilieff, E., Karas, M., and Schägger, H. (2007). Identification of two proteins associated with mammalian ATP synthase. Mol Cell Proteomics 6(10), 1690–1699.

Mitra, S.K., Gantt, J.A., Ruby, J.F., Clouse, S.D., Goshe, M.B. (2007). Membrane proteomic analysis of Arabidopsis thaliana using alternative solubilization techniques. J Proteome Res 6, 1933–1950.

Mitra, S.K., Walters, B.T., Clouse, S.D., and Goshe, M.B. (2009). An efficient organic solvent based extraction method for the proteomic analysis of Arabidopsis plasma membranes. J Proteome Res 8, 2752–2767.

Moebius, J., Zahedi, R.P., Lewandrowski, U., Berger, C., Walter, U., and Sickmann, A. (2005). The human platelet membrane proteome reveals several new potential membrane proteins. Mol Cell Proteomics 4, 1754–1761.

Mohnen, D. (2008). Pectin structure and biosynthesis. Curr Opin Plant Biol 11, 266–277.

Morel, J., Claverol, S., Mongrand, S., Furt, F., Fromentin, J., Bessoule, J.J., Blein, J.P., and Simon-Plas, F. (2006). Proteomics of plant detergent-resistant membranes. Mol Cell Proteomics 5, 1396–1411.

Myung, J.K., and Lubec, G. (2006). Use of solution-IEF-fractionation leads to separation of 2673 mouse brain proteins including 255 hydrophobic structures. J Proteome Res 5, 1267–1275.

Nagaraj, N., Lu, A.P., Mann, M., and Wisniewski, J.R. (2008). Detergent-based but gel-free method allows identification of several hundred membrane proteins in single LC-MS runs. J Proteome Res 7, 5028–5032.

Neff, D., and Dencher, N.A. (1999). Purification of multisubunit membrane protein complexes: Isolation of chloroplast FoF1-ATP synthase, CFo and CF1 by blue native electrophoresis. Biochem Biophys Res Commun 259, 569–575.

Nelson, C.J., Hegeman, A.D., Harms, A.C., and Sussman, M.R. (2006). A quantitative analysis of Arabidopsis plasma membrane using trypsin-catalyzed O-18 labeling. Mol Cell Proteomics 5, 1382–1395.

Nilsson, R., Bernfur, K., Gustavsson, N., Bygdell, J., Wingsle, G., and Larsson, C. (2010). Proteomics of plasma membranes from poplar trees reveals tissue distribution of transporters, receptors and proteins in cell wall formation. Mol Cell Proteomics 9, 368–387.

Nirmalan, N., Flett, F., Skinner, T., Hyde, J.E., and Sims, P.F.G. (2007). Microscale solution lsoelectric focusing as an effective strategy enabling containment of hemeoglobin-derived products for high-resolution gel-based analysis of the Plasmodium falciparum proteome. J Proteome Res 6, 3780–3787.

Nühse, T.S., Bottrill, A.R., Jones, A.M.E., and Peck, S.C. (2007). Quantitative phosphoproteomic analysis of plasma membrane proteins reveals regulatory mechanisms of plant innate immune responses. Plant J 51, 931–940.

Nühse, T.S., Stensballe, A, Jensen, O.N., and Peck, S.C. (2004). Phosphoproteomics of the Arabidopsis plasma membrane and a new phosphorylation site database. Plant Cell 16, 2394–2405.

Pedersen, S.K., Harry, J.L., Sebastian, L, Baker, J., Traini, M.D., McCarthy, J.T., Manoharan, A., Wilkins, M.R., Gooley, A.A., Righetti, P.G., Packer, N.H., Williams, K.L., and Herbert, B.R. (2003). Unseen proteome: Mining below the tip of the iceberg to find low abundance and membrane proteins. J Proteome Res 2, 303–311.

Persson, A., and Jergil, B. (1994). Rat liver plasma membranes. Methods Enzymol 228, 489–496.

Persson, A., Johansson, B., Olsson, H., and Jergil, B. (1991). Purification of rat liver plasma membranes by wheat-germ-agglutinin affinity partitioning. Biochem J 273, 173–177.

Poetsch, A., Neff, D., Seelert, H., Schägger, H., and Dencher, N.A. (2000). Dye removal, catalytic activity and 2D crystallization of chloroplast H⁺-ATP synthase purified by blue native electrophoresis. Biochim Biophys Acta 1466, 339–349.

Prime, T.A., Sherrier, D.J., Mahon, P, Packman, L.C., and Dupree, P. (2000). A proteomic analysis of organelles from Arabidopsis thaliana. Electrophoresis 21, 3488–3499.

Rais, I., Karas, M., and Schägger, H. (2004). Two-dimensional electrophoresis for the isolation of integral membrane proteins and mass spectrometric identification. Proteomics 4, 2567–2571.

Reisinger, V., and Eichacker, L.A. (2006). Analysis of membrane protein complexes by blue native PAGE. Proteomics 6(Suppl 2), 6–15.

Righetti, P.G., Citterio, A., and Girault, H. (2007). Gel-free IEF in a membrane-sealed multicompartment cell for proteome prefractionation. Electrophoresis 28, 1860–1866.

Rolland, N., Ferro, M., Ephritikhine, G., Marmagne, A., Ramus, C., Brugière, S., Salvi, D., Seigneurin-Berny, D., Bourguignon, J., Barbier-Brygoo, H., Joyard, J., and Garin, J. (2006). A versatile method for deciphering plant membrane proteomes. J Exp Bot 57, 1579–1589.

Ross, P.L., Huang, Y.L.N., Marchese, J.N., Williamson, B., Parker, K., Hattan, S., Khainovski, N., Pillai, S., Dey, S., Daniels, S., Purkayastha, S., Juhasz, P., Martin, S., Bartlet-Jones, M., He, F., Jacobson, A., and Pappin, D.J. (2004). Multiplexed protein quantitation in Saccharomyces cerevisiae using amine-reactive isobaric tagging reagent. Mol Cell Proteomics 3, 1154–1169.

Santoni, V. (2007). Plant plasma membrane protein extraction and solubilization for proteomic analysis. Methods Mol Biol 335, 93–109.

Santoni, V., Doumas, P., Rouquié, D., Mansion, M., Rabilloud, T., and Rossignol, M. (1999). Large scale characterization of plant plasma membrane proteins. Biochimie 81, 655–661.

Santoni, V., Kieffer, S., Desclaux, D., Masson, F., and Rabilloud, T. (2000). Membrane proteomics: Use of additive main effects with multiplicative interaction model to classify plasma membrane proteins according to their solubility and electrophoretic properties. Electrophoresis 21, 3329–3344.

Santoni, V., Molloy, M., and Rabilloud, T. (2000). Membrane proteins and proteomics: Un amour impossible? Electrophoresis 21, 1054–1070.

Santoni, V., Rouquié, D., Doumas, P., Mansion, M., Boutry, M., Degand, H., Dupree, P., Packman, L., Sherrier, J., Prime, T., Bauw, G., Posada, E., Rouzé, P., Dehais, P., Sahnoun, I., Barlier, I., and Rossignol, M. (1998). Use of a proteome strategy for tagging proteins present at the plasma membrane. Plant J 16, 633–641.

Santoni, V., Vinh, J., Pflieger, D., Sommerer, N., and Maurel, C. (2003). A proteomic study reveals novel insights into the diversity of aquaporin forms expressed in the plasma membrane of plant roots. Biochem J 373, 289–296.

Sarkar, P., Bosneaga, E., and Auer, M. (2009). Plant cell walls throughout evolution: towards a molecular understanding of their design principles. J Exp Bot 60, 3615–3635.

Schägger, H., and Pfeiffer, K. (2000). Supercomplexes in the respiratory chains of yeast and mammalian mitochondria. EMBO J 19, 1777–1783.

Schägger, H., Cramer, W.A., and von Jagow, G. (1994). Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal Biochem 217, 220–230.

Schägger, H., and von Jagow, G. (1987). Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166, 368–379.

Schägger, H., and von Jagow, G. (1991). Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199, 223–231.

Schindler, J., and Nothwang, H.G. (2006). Aqueous polymer two-phase systems: Effective tools for plasma membrane proteomics. Proteomics 6, 5409–5417.

Schulenberg, B., and Patton, W.F. (2004). Combining microscale solution-phase isoelectric focusing with multiplexed Proteomics((R)) dye staining to analyze protein post-translational modifications. Electrophoresis 25, 2539–2544.

Simões-Barbosa, A., Santana, J.M., and Teixeira, A.R. (2000). Solubilization of delipidated macrophage membrane proteins for analysis by two-dimensional electrophoresis. Electrophoresis 21, 641–644.

Speers, A.E., and Wu, C.C. (2007). Proteomics of integral membrane proteins--theory and application. Chem Rev 107, 3687–3714.

Stanislas, T., Bouyssie, D., Rossignol, M., Vesa, S., Fromentin, J., Morel, J., Pichereaux, C., Monsarrat, B., and Simon-Plas, F. (2009). Quantitative proteomics reveals a dynamic association of proteins to detergent-resistant membranes upon elicitor signaling in tobacco. Mol Cell Proteomics 8, 2186–2198.

Tanaka, N., Fujita, M., Handa, H., Murayama, S., Uemura, M., Kawamura, Y., Mitsui, T., Mikami, S., Tozawa, Y., Yoshinaga, T., and Komatsu, S. (2004). Proteomics of the rice cell: Systematic identification of the protein populations in subcellular compartments. Mol Genet Genomics 271, 566–576.

Tang, W., Deng, Z., Oses-Prieto, J.A., Suzuki, N., Zhu, S., Zhang, X., Burlingame, A.L., and Wang, Z.Y. (2008). Proteomics studies of brassinosteroid signal transduction using prefractionation and two-dimensional DIGE. Mol Cell Proteomics 7, 728–738.

Tastet, C., Charmont, S., Chevallet, M., Luche, S., and Rabilloud, T. (2003). Structure-efficiency relationships of zwitterionic detergents as protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 111–121.

Toll, E., Castillo, F.J., Crespi, P., Crevecoeur, M., and Greppin, H. (1995). Purification of plasma membranes from leaves of conifer and deciduous tree species by phase partitioning and free-flow electrophoresis. Physiol Plant 95, 399–408.

Valot, B., Negroni, L., Zivy, M., Gianinazzi, S., and Dumas-Gaudot, E. (2006). A mass spectrometric approach to identify arbuscular mycorrhiza-related proteins in root plasma membrane fractions. Proteomics 6, 145–155.

Wang, N., Young, J.B., and Li, L. (2009). Liquid chromatography MALDI MS/MS for membrane proteome analysis. Methods Mol Biol 528, 295–231.

Weber, G., Islinger, M., Weber, P., Eckerskorn, C., and Völkl, A. (2004). Efficient separation and analysis of peroxisomal membrane proteins using free-flow isoelectric focusing. Electrophoresis 25, 1735–1747.

Wenge, B., Bönisch, H., Grabitzki, J., Lochnit, G., Schmitz, B., and Ahrend, M.H. (2008). Separation of membrane proteins by two-dimensional electrophoresis using cationic rehydrated strips. Electrophoresis 29, 1511–1517.

Wessel, D., and Flügge, U.I. (1984). A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Anal Biochem 138, 141–143.

Williams, T.I., Combs, J.C., Thakur, A.P., Strobel, H.J., and Lynn, B.C. (2006). A novel Bicine runningbuffer system for doubled sodium dodecyl sulfate – polyacrylamide gel electrophoresis of membrane proteins. Electrophoresis 27, 2984–2995.

Wittig, I., Braun, H.P., and Schägger, H. (2006). Blue native PAGE. Nat Protoc 1, 418–428.

Wumaier, Z., Nübel, E., Wittig, I., and Schägger, H. (2009). Chapter 8: Two-dimensional native electrophoresis for fluorescent and functional assays of mitochondrial complexes. Methods Enzymol 456, 153–168.

Zahedi, R.P., Meisinger, C., and Sickmann, A. (2005). Two-dimensional benzyldimethyl-n-hexadecylammonium chloride/SDS-PAGE for membrane proteomics. Proteomics 5, 3581–3588.

Zhang, L.J., Wang, X.E., Peng, X., Wei, Y.J., Cao, R., Liu, Z., Xiong, J.X., Yin, X.F., Ping, C., and Liang, S. (2006). Proteomic analysis of low-abundant integral plasma membrane proteins based on gels. Cell Mol Life Sci 63, 1790–804.

Zappel, N.F., and Panstruga, R. (2008). Heterogeneity and lateral compartmentalization of plant plasma membranes. Curr Opin Plant Biol 11, 632–640.

Title: Plant Plasma Membrane Proteomics: Challenges and Possibilities
Authors: Anders Laurell Blom Møller
Katja Witzel
Annelies Vertommen
Vibeke Barkholt
Birte Svensson
Sebastien Carpentier
Hans-Peter Mock
Christine Finnie
Publisher: Springer Netherlands
Book: Sample Preparation in Biological Mass Spectrometry
Print ISBN: 978-94-007-0758-0

Electronic ISBN: 978-94-007-0828-0

Copyright Year: 2011
DOI: https://doi.org/10.1007/978-94-007-0828-0_21

Springer Professional

Abstract

Please log in to get access to your license.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Springer Professional "Technik"

Premium Partners