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2011 | OriginalPaper | Chapter

20. Efficient Strategies for Analysis of Low Abundance Proteins in Plant Proteomics

Authors : Olga A. Koroleva, Laurence V. Bindschedler

Published in: Sample Preparation in Biological Mass Spectrometry

Publisher: Springer Netherlands

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Abstract

Proteomic studies alongside with other post-genomic approaches are rapidly progressing in plant biology. This trend is reflected by the increased number of publications related to plant proteomics in the last several years. While proteomic studies span from the global characterisation of proteomes (the protein complements) in whole plants and organs to the more detailed characterisation of particular proteins, there is an increasing demand for methods allowing sophisticated (and preferably quantitative) analysis of a variety of cells, compartments and organelles, post-translational protein modifications, protein complexes and their dynamics. The common problem is the high dynamic range of the proteome, and many researchers are looking for the ways to improve the recovery of naturally low-abundant plant proteins. The aim of this chapter is to provide a review of a variety of approaches, rather than specific protocols, established for the isolation of specific fractions of proteins suitable for analysis by mass spectrometry. We focus on the sample preparation methodology from general to specific applications in plant proteomics and on advances made in the characterisation of global plant proteomes and plant-specific compartments. We also describe the advantages of metabolic labeling techniques suited for quantitative plant proteomic studies and discuss tandem affinity purification of plant protein complexes and perspectives of single-cell proteomics in plants.

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Title: Efficient Strategies for Analysis of Low Abundance Proteins in Plant Proteomics
Authors: Olga A. Koroleva
Laurence V. Bindschedler
Publisher: Springer Netherlands
Book: Sample Preparation in Biological Mass Spectrometry
Print ISBN: 978-94-007-0758-0

Electronic ISBN: 978-94-007-0828-0

Copyright Year: 2011
DOI: https://doi.org/10.1007/978-94-007-0828-0_20

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