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Topics in Catalysis
Published in: Topics in Catalysis 17-20/2014

01-11-2014 | Original Paper

Porous Poly(vinyl alcohol) Composite Membranes for Immobilization of Glucose Oxidase

Authors: Ernesto Silva Mojica, Mehdi Lohrasbi, Steven S. C. Chuang

Published in: Topics in Catalysis | Issue 17-20/2014

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Abstract

Particle loaded porous poly(vinyl alcohol) composite membranes were selected for immobilization of glucose oxidase (GOx) for their hydrophilicity and unique interactions with amino functional groups. GOx was immobilized on the membranes by adsorption at pH values between 3.5 and 7.1. The highest adsorption loading was observed at pH 7.1 and the highest catalytic activity was observed at pH 5.1. Infrared studies showed that the highest ratio of amide I to amide II at pH 5.1 is obtained for GOx immobilized on membranes loaded with amine-functionalized micro-particles, suggesting that the conformational changes of GOx on these membranes yield to higher catalytic activity than in other supports.
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Metadata
Title
Porous Poly(vinyl alcohol) Composite Membranes for Immobilization of Glucose Oxidase
Authors
Ernesto Silva Mojica
Mehdi Lohrasbi
Steven S. C. Chuang
Publication date
01-11-2014
Publisher
Springer US
Published in
Topics in Catalysis / Issue 17-20/2014
Print ISSN: 1022-5528
Electronic ISSN: 1572-9028
DOI
https://doi.org/10.1007/s11244-014-0323-3

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