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03-01-2021

Response of Pseudomonas tolaasii, the causal agent of mushroom brown blotch disease to the volatile compounds produced by endofungal bacteria

Authors: Samira Ghasemi, Behrouz Harighi, Mahdi Mojarrab, Abdolbaset Azizi

Published in: BioControl | Issue 3/2021

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Abstract

Volatile organic compounds (VOCs) produced by bacteria have significant potential to control phytopathogens. In this study, the VOCs produced by endofungal bacteria Pseudomonas sp. Bi1, Bacillus sp. De3, Pantoea sp. Ma3 and Pseudomonas sp. De1 isolated from wild growing mushrooms were evaluated in vitro for their antagonistic activity against Pseudomonas tolaasii Pt18, the causal agent of mushroom brown blotch disease. The gas chromatography–mass spectrometry (GC–MS) analysis revealed that strains Pseudomonas sp. Bi1, Pseudomonas sp. De1, Bacillus sp. De3 and Pantoea sp. Ma3 produced eight, sixteen, nine, and twelve VOCs, respectively. All antagonistic endofungal bacteria produced VOCs which significantly reduced brown blotch symptoms on mushroom caps and inhibited the growth of P. tolaasii Pt18 at the varying levels. Scanning electron microscopy revealed severe morphological changes in cells of P. tolaasii Pt18 following exposure to the VOCs of Pseudomonas sp. Bi1 and De1. Furthermore, The VOCs produced by endofungal bacteria significantly reduced swarming, swimming, twitching, chemotaxis motility and biofilm formation by P. tolaasii Pt18 cells, which are essential contributors to pathogenicity. This is to first report about the inhibition effects of VOCs produced by antagonistic bacteria on virulence traits of P. tolaasii. Our findings provide new insights regarding the potential of antibacterial VOCs as a safe fumigant to control mushroom brown blotch disease.

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Title: Response of Pseudomonas tolaasii, the causal agent of mushroom brown blotch disease to the volatile compounds produced by endofungal bacteria
Authors: Samira Ghasemi
Behrouz Harighi
Mahdi Mojarrab
Abdolbaset Azizi
Publication date: 03-01-2021
Publisher: Springer Netherlands
Published in: BioControl / Issue 3/2021
Print ISSN: 1386-6141
Electronic ISSN: 1573-8248
DOI: https://doi.org/10.1007/s10526-020-10071-6

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