Skip to main content
Top
Published in: Experiments in Fluids 10/2021

01-10-2021 | Research Article

Rhodamine-based caged dye for aqueous MTV with green lasers

Authors: Charles Fort, Philippe M. Bardet

Published in: Experiments in Fluids | Issue 10/2021

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Molecular tagging velocimetry (MTV) is a nonintrusive optical diagnostic with typically three variants in liquids distinguished by the dye that they rely on: phosphorescent, photochromic, or caged. The latter scheme, initially called photo-activated nonintrusive tracking of molecular motion (PHANTOMM), required both a UV and a dedicated blue laser. Here, we demonstrate caged-dye-based MTV with pulsed UV and green lasers for the first time, simplifying its deployment in most experimental fluid dynamics laboratories.

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

Literature
go back to reference Biage M, Campos JCC (2003) Visualization study and quantitative velocity measurements in turbulent taylor-couette flow by phantomm flow tagging: a description of the transition to turbulence. J Braz Soc Mech Sci 25(4):378–390CrossRef Biage M, Campos JCC (2003) Visualization study and quantitative velocity measurements in turbulent taylor-couette flow by phantomm flow tagging: a description of the transition to turbulence. J Braz Soc Mech Sci 25(4):378–390CrossRef
go back to reference Bonin K, Smelser A, Moreno NS, Holzwarth G, Wang K, Levy P, Vidi P-A (2018) Structured illumination to spatially map chromatin motions. J Biomed Opt 23(5):056007CrossRef Bonin K, Smelser A, Moreno NS, Holzwarth G, Wang K, Levy P, Vidi P-A (2018) Structured illumination to spatially map chromatin motions. J Biomed Opt 23(5):056007CrossRef
go back to reference Dussaud AD, Troian SM, Harris SR (1998) Fluorescence visualization of a convective instability which modulates the spreading of volatile surface films. Phys Fluids 10(7):1588–1596CrossRef Dussaud AD, Troian SM, Harris SR (1998) Fluorescence visualization of a convective instability which modulates the spreading of volatile surface films. Phys Fluids 10(7):1588–1596CrossRef
go back to reference Fort C, Bardet PM (2021) Efficient photobleaching of rhodamine 6G by a single UV pulse. Appl Opt 60(22):6342–6350CrossRef Fort C, Bardet PM (2021) Efficient photobleaching of rhodamine 6G by a single UV pulse. Appl Opt 60(22):6342–6350CrossRef
go back to reference Fort C, André MA, Bardet PM (2020a) Development of long distance 2D micro-molecular tagging velocimetry (\(\mu \)MTV) to measure wall shear stress. In: AIAA Scitech Forum, p 1274 Fort C, André MA, Bardet PM (2020a) Development of long distance 2D micro-molecular tagging velocimetry (\(\mu \)MTV) to measure wall shear stress. In: AIAA Scitech Forum, p 1274
go back to reference Fort C, André MA, Pazhand H, Bardet PM (2020b) Talbot-effect structured illumination: pattern generation and application to long-distance \(\upmu \)-MTV. Exp Fluids 61(2):40CrossRef Fort C, André MA, Pazhand H, Bardet PM (2020b) Talbot-effect structured illumination: pattern generation and application to long-distance \(\upmu \)-MTV. Exp Fluids 61(2):40CrossRef
go back to reference Gee KR, Weinberg ES, Kozlowski DJ (2001) Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer. Bioorg Med Chem Lett 11(16):2181–2183CrossRef Gee KR, Weinberg ES, Kozlowski DJ (2001) Caged Q-rhodamine dextran: a new photoactivated fluorescent tracer. Bioorg Med Chem Lett 11(16):2181–2183CrossRef
go back to reference Guilkey JE, Gee KR, McMurtry PA, Klewicki JC (1996) Use of caged fluorescent dyes for the study of turbulent passive scalar mixing. Exp Fluids 21(4):237–242CrossRef Guilkey JE, Gee KR, McMurtry PA, Klewicki JC (1996) Use of caged fluorescent dyes for the study of turbulent passive scalar mixing. Exp Fluids 21(4):237–242CrossRef
go back to reference Harris SR (1999) Quantitative measurements in a lid-driven, cylindrical cavity using the PHANTOMM flow-tagging technique. PhD thesis, Princeton University Harris SR (1999) Quantitative measurements in a lid-driven, cylindrical cavity using the PHANTOMM flow-tagging technique. PhD thesis, Princeton University
go back to reference Hauke S, von Appen A, Quidwai T, Ries J, Wombacher R (2017) Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells. Chem Sci 8(1):559–566CrossRef Hauke S, von Appen A, Quidwai T, Ries J, Wombacher R (2017) Specific protein labeling with caged fluorophores for dual-color imaging and super-resolution microscopy in living cells. Chem Sci 8(1):559–566CrossRef
go back to reference Jasuja R, Keyoung J, Reid GP, Trentham DR, Khan S (1999) Chemotactic responses of Escherichia coli to small jumps of photoreleased l-aspartate. Biophys J 76(3):1706–1719CrossRef Jasuja R, Keyoung J, Reid GP, Trentham DR, Khan S (1999) Chemotactic responses of Escherichia coli to small jumps of photoreleased l-aspartate. Biophys J 76(3):1706–1719CrossRef
go back to reference Johnson ID (2006) Practical considerations in the selection and application of fluorescent probes. Handbook of biological confocal microscopy. Springer, Boston, pp 353–367CrossRef Johnson ID (2006) Practical considerations in the selection and application of fluorescent probes. Handbook of biological confocal microscopy. Springer, Boston, pp 353–367CrossRef
go back to reference Klán P, Solomek T, Bochet CG, Blanc A, Givens R, Rubina M, Popik V, Kostikov A, Wirz J (2012) Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy. Chem Rev 113(1):119–191CrossRef Klán P, Solomek T, Bochet CG, Blanc A, Givens R, Rubina M, Popik V, Kostikov A, Wirz J (2012) Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy. Chem Rev 113(1):119–191CrossRef
go back to reference Koochesfahani MM, Nocera DG (2007) Molecular tagging velocimetry. In: Tropea C, Yarin A, Foss J (eds) Handbook of experimental fluid dynamics, Chap. 5.4. Springer, Berlin, pp 362–382 Koochesfahani MM, Nocera DG (2007) Molecular tagging velocimetry. In: Tropea C, Yarin A, Foss J (eds) Handbook of experimental fluid dynamics, Chap. 5.4. Springer, Berlin, pp 362–382
go back to reference Kozma E, Kele P (2019) Fluorogenic probes for super-resolution microscopy. Organ Biomol Chem 17(2):215–233CrossRef Kozma E, Kele P (2019) Fluorogenic probes for super-resolution microscopy. Organ Biomol Chem 17(2):215–233CrossRef
go back to reference Lempert WR, Ronney P, Magee K, Gee KR, Haugland RP (1995) Flow tagging velocimetry in incompressible flow using photo-activated nonintrusive tracking of molecular motion (phantomm). Exp Fluids 18(4):249–257CrossRef Lempert WR, Ronney P, Magee K, Gee KR, Haugland RP (1995) Flow tagging velocimetry in incompressible flow using photo-activated nonintrusive tracking of molecular motion (phantomm). Exp Fluids 18(4):249–257CrossRef
go back to reference Lempert WR, Lee D, Harris SR, Miles RB, Gee KR (1998) Synthesis and characterization of new caged dye velocimetry tracers for microgravity droplet diagnostics 27: 512 Lempert WR, Lee D, Harris SR, Miles RB, Gee KR (1998) Synthesis and characterization of new caged dye velocimetry tracers for microgravity droplet diagnostics 27: 512
go back to reference Li W-H, Zheng G (2012) Photoactivatable fluorophores and techniques for biological imaging applications. Photochem Photobiol Sci 11(3):460–471MathSciNetCrossRef Li W-H, Zheng G (2012) Photoactivatable fluorophores and techniques for biological imaging applications. Photochem Photobiol Sci 11(3):460–471MathSciNetCrossRef
go back to reference Lohse S, Kohnen BT, Janasek D, Dittrich PS, Franzke J, Agar DW (2008) A novel method for determining residence time distribution in intricately structured microreactors. Lab Chip 8(3):431–438CrossRef Lohse S, Kohnen BT, Janasek D, Dittrich PS, Franzke J, Agar DW (2008) A novel method for determining residence time distribution in intricately structured microreactors. Lab Chip 8(3):431–438CrossRef
go back to reference Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A (1998) [4] caged fluorescent probes. Methods Enzymol 291:63–78CrossRef Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A (1998) [4] caged fluorescent probes. Methods Enzymol 291:63–78CrossRef
go back to reference Molho JI, Herr AE, Mosier BP, Santiago JG, Kenny TW, Brennen RA, Gordon GB, Mohammadi B (2001) Optimization of turn geometries for microchip electrophoresis. Anal Chem 73(6):1350–1360CrossRef Molho JI, Herr AE, Mosier BP, Santiago JG, Kenny TW, Brennen RA, Gordon GB, Mohammadi B (2001) Optimization of turn geometries for microchip electrophoresis. Anal Chem 73(6):1350–1360CrossRef
go back to reference Park JS, Kim HJ, Kihm KD (2001) Molecular tagging fluorescence velocimetry (mtfv) for lagrangian flow field mapping inside evaporating meniscus: potential use for microscale applications. J Flow Visual Image Process 8:177–187 Park JS, Kim HJ, Kihm KD (2001) Molecular tagging fluorescence velocimetry (mtfv) for lagrangian flow field mapping inside evaporating meniscus: potential use for microscale applications. J Flow Visual Image Process 8:177–187
go back to reference Park JS, McCarty C, Kihm KD, Pratt DM (2000) Lagrangian flow mapping of heated capillary pore and thin film using molecular fluorescence velocimetry (MFV). J Heat Transf 122(3):423CrossRef Park JS, McCarty C, Kihm KD, Pratt DM (2000) Lagrangian flow mapping of heated capillary pore and thin film using molecular fluorescence velocimetry (MFV). J Heat Transf 122(3):423CrossRef
go back to reference Paul PH, Garguilo MG, Rakestraw DJ (1998) Imaging of pressure-and electrokinetically driven flows through open capillaries. Anal Chem 70(13):2459–2467CrossRef Paul PH, Garguilo MG, Rakestraw DJ (1998) Imaging of pressure-and electrokinetically driven flows through open capillaries. Anal Chem 70(13):2459–2467CrossRef
go back to reference Payumo AY, Walker WJ, McQuade LE, Yamazoe S, Chen JK (2015) Optochemical dissection of t-box gene-dependent medial floor plate development. ACS Chem Biol 10(6):1466–1475CrossRef Payumo AY, Walker WJ, McQuade LE, Yamazoe S, Chen JK (2015) Optochemical dissection of t-box gene-dependent medial floor plate development. ACS Chem Biol 10(6):1466–1475CrossRef
go back to reference Pelliccioli AP, Wirz J (2002) Photoremovable protecting groups: reaction mechanisms and applications. Photochem Photobiol Sci 1(7):441–458CrossRef Pelliccioli AP, Wirz J (2002) Photoremovable protecting groups: reaction mechanisms and applications. Photochem Photobiol Sci 1(7):441–458CrossRef
go back to reference Roetmann K, Schmunk W, Garbe CS, Beushausen V (2008) Micro-flow analysis by molecular tagging velocimetry and planar Raman-scattering. Exp Fluids 44(3):419–430CrossRef Roetmann K, Schmunk W, Garbe CS, Beushausen V (2008) Micro-flow analysis by molecular tagging velocimetry and planar Raman-scattering. Exp Fluids 44(3):419–430CrossRef
go back to reference Ross D, Johnson TJ, Locascio LE (2001) Imaging of electroosmotic flow in plastic microchannels. Anal Chem 73(11):2509–2515CrossRef Ross D, Johnson TJ, Locascio LE (2001) Imaging of electroosmotic flow in plastic microchannels. Anal Chem 73(11):2509–2515CrossRef
go back to reference Sawin KE, Mitchison TJ (1999) Photoactivation of fluorescence as a probe for cytoskeletal dynamics. In: Watson W (ed) Mitosis and cell motility, in Fluorescent and luminescent probes for biological activity. Academic Press, San Diego, USA, pp 613–627CrossRef Sawin KE, Mitchison TJ (1999) Photoactivation of fluorescence as a probe for cytoskeletal dynamics. In: Watson W (ed) Mitosis and cell motility, in Fluorescent and luminescent probes for biological activity. Academic Press, San Diego, USA, pp 613–627CrossRef
go back to reference Schuster BS, Allan DB, Kays JC, Hanes J, Leheny RL (2017) Photoactivatable fluorescent probes reveal heterogeneous nanoparticle permeation through biological gels at multiple scales. J Control Release 260:124–133CrossRef Schuster BS, Allan DB, Kays JC, Hanes J, Leheny RL (2017) Photoactivatable fluorescent probes reveal heterogeneous nanoparticle permeation through biological gels at multiple scales. J Control Release 260:124–133CrossRef
go back to reference Shelby JP, Chiu DT (2003) Mapping fast flows over micrometer-length scales using flow-tagging velocimetry and single-molecule detection. Anal Chem 75(6):1387–1392CrossRef Shelby JP, Chiu DT (2003) Mapping fast flows over micrometer-length scales using flow-tagging velocimetry and single-molecule detection. Anal Chem 75(6):1387–1392CrossRef
go back to reference Sinton D, Erickson D, Li D (2002) Photo-injection based sample design and electroosmotic transport in microchannels. J Micromech Microeng 12(6):898CrossRef Sinton D, Erickson D, Li D (2002) Photo-injection based sample design and electroosmotic transport in microchannels. J Micromech Microeng 12(6):898CrossRef
go back to reference Wysocki LM, Grimm JB, Tkachuk AN, Brown TA, Betzig E, Lavis LD (2011) Facile and general synthesis of photoactivatable xanthene dyes. Angew Chem Int Edn 50(47):11206–11209CrossRef Wysocki LM, Grimm JB, Tkachuk AN, Brown TA, Betzig E, Lavis LD (2011) Facile and general synthesis of photoactivatable xanthene dyes. Angew Chem Int Edn 50(47):11206–11209CrossRef
go back to reference Xia P, Bungay PM, Gibson CC, Kovbasnjuk ON, Spring KR (1998) Diffusion coefficients in the lateral intercellular spaces of madin-darby canine kidney cell epithelium determined with caged compounds. Biophys J 74(6):3302–3312CrossRef Xia P, Bungay PM, Gibson CC, Kovbasnjuk ON, Spring KR (1998) Diffusion coefficients in the lateral intercellular spaces of madin-darby canine kidney cell epithelium determined with caged compounds. Biophys J 74(6):3302–3312CrossRef
go back to reference Zhao Y, Zheng Q, Dakin K, Xu K, Martinez ML, Li W-H (2004) New caged coumarin fluorophores with extraordinary uncaging cross sections suitable for biological imaging applications. J Am Chem Soc 126(14):4653–4663CrossRef Zhao Y, Zheng Q, Dakin K, Xu K, Martinez ML, Li W-H (2004) New caged coumarin fluorophores with extraordinary uncaging cross sections suitable for biological imaging applications. J Am Chem Soc 126(14):4653–4663CrossRef
Metadata
Title
Rhodamine-based caged dye for aqueous MTV with green lasers
Authors
Charles Fort
Philippe M. Bardet
Publication date
01-10-2021
Publisher
Springer Berlin Heidelberg
Published in
Experiments in Fluids / Issue 10/2021
Print ISSN: 0723-4864
Electronic ISSN: 1432-1114
DOI
https://doi.org/10.1007/s00348-021-03303-7

Other articles of this Issue 10/2021

Experiments in Fluids 10/2021 Go to the issue

Premium Partners