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Published in:
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2015 | Online First | Chapter

Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells

Authors : Cristian Miere, Liani Devito, Dusko Ilic

Published in: Methods in Molecular Biology™

Publisher: Springer New York

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Abstract

In an attempt to bring pluripotent stem cell biology closer to reaching its full potential, many groups have focused on improving reprogramming protocols over the past several years. The episomal modified Sendai virus-based vector has emerged as one of the most practical ones. Here we describe reprogramming of mesenchymal stromal/stem cells (MSC) derived from umbilical cord Wharton’s Jelly into induced pluripotent stem cells (iPSC) using genome non-integrating Sendai virus-based vectors. The detailed protocols of iPSC colony cryopreservation (vitrification) and adaption to feeder-free culture conditions are also included.
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Metadata
Title
Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells
Authors
Cristian Miere
Liani Devito
Dusko Ilic
Copyright Year
2015
Publisher
Springer New York
DOI
https://doi.org/10.1007/7651_2014_163