Skip to main content
Disciplines
Automotive Business IT + Informatics Construction + Real Estate Electrical Engineering + Electronics Energy + Sustainability Insurance + Risk Finance + Banking Management + Leadership Marketing + Sales Mechanical Engineering + Materials
Events
DE
EN
Books
Journals
Topic Page
Marketing
Start single access now
Access for companies
EXTENDED SEARCH
Log in
Top
Journal of Materials Science
Published in: Journal of Materials Science 9/2015

01-05-2015 | Original Paper

SERS tags-based novel monodispersed hollow gold nanospheres for highly sensitive immunoassay of CEA

Authors: Mingde Guo, Jian Dong, Wei Xie, Lin Tao, Wenbo Lu, Ying Wang, Weiping Qian

Published in: Journal of Materials Science | Issue 9/2015

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Here, a highly specific, sensitive, and reliable immunoassay has been developed based on surface-enhanced Raman scattering for quantitative detection of carcinoembryonic antigen (CEA). A novel hollow gold nanospheres (HGNs) was firstly fabricated by seed-mediated methods instead of the one-step methods with high homogeneity and then, the monodispersed HGNs were embedded with 4-mercaptobenzonic acid to form surface-enhanced Raman scattering (SERS) tags and magnetic microspheres were synthesized as enrichment substrates. Based on this sandwich immunoassay strategy, CEA monoclonal antibody -conjugated HGNs-based SERS tags and CEA polyclonal antibodies -conjugated magnetic microspheres were used to detect CEA up to 100 ng/mL with a detection limit of 10 pg/mL, which were two orders of magnitudes lower than that of GNSs-based SERS tags. The monodispersed HGNs were desirable SERS tags for quantitative sandwich immunoassay assay, showing strong potential for the clinical diagnosis of cancer biomarkers.
previous article Microstructure and properties of TiB2 particles reinforced Cu–Cr matrix composite
next article Liquid phase combustion of iron in an oxygen atmosphere

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

inform now
Appendix
Available only for authorised users
Literature
1.
Rasooly A, Jacobson J (2006) Development of biosensors for cancer clinical testing. Biosens Bioelectron 21:1851–1858CrossRef
2.
Tothill IE (2009) Biosensors for cancer markers diagnosis. Semin Cell Dev Biol 20:55–62CrossRef
3.
Mohd FU, Mohammad A (2009) The footprints of cancer development: cancer biomarkers. Cancer Treat Rev 35:193–200CrossRef
4.
Wu J, Fu ZF, Yan F, Ju HX (2008) Biomedical and clinical applications of immunoassays and immunosensors for tumor markers. Trends Anal Chem 26:679–688CrossRef
5.
Sung HJ, Cho JY (2008) Biomarkers for the lung cancer diagnosis and their advances in proteomics. BMB Rep 41(9):615–625CrossRef
6.
Sutedja TG, Venmans BJ, Smit EF, Postmus PE (2007) Fluorescence bronchoscopy for early detection of lung cancer: a clinical perspective. Lung Cancer 34:157–168CrossRef
7.
Peter JM, Jeffrey H, Raed D, Jie N, Carmen C, Daniel L, Tarek M (2007) Diagnosis of lung cancer by the analysis of exhaled breath with a colorimetric sensor array. Lung Cancer 62:565–568
8.
Danqun Huo, Yonghong Xu, Changjun Hou, Huanbao Fa (2014) A novel optical chemical sensor based AuNR-MTPP and dyes for lungcancer biomarkers in exhaled breath identification. Sens Actuators B 199:446–456CrossRef
9.
Bharti A, Ma PC, Salgia R (2007) Biomarker discovery in lung cancer—promises and challenges of clinical proteomics. Mass Spectrom Rev 26:451–466CrossRef
10.
Pothur RS, Barnett SK, Sudhir S (2001) Trends in biomarker research for cancer detection. Lancet Oncol 2:698–704CrossRef
11.
Greenberga AK, Lee MS (2007) Biomarkers for lung cancer: clinical uses. Curr Opin Pulm Med 13:249–255CrossRef
12.
Chon H, Lee S, Son SW, Oh CH, Choo J (2009) Highly sensitive immunoassay of lung cancer marker carcinoembryonic antigen using surface-enhanced Raman scattering of hollow gold nanospheres. Anal Chem 81:3029–3034CrossRef
13.
Gong JL, Liang Y, Huang YY, Chen JW, Jiang JH, Shen GL, Yu RQ (2007) Ag/SiO2 core-shell nanoparticle-based surface-enhanced Raman probes for immunoassay of cancer marker using silica-coated magnetic nanoparticles as separation tools. Biosens Bioelectron 22:1501–1507CrossRef
14.
Chon H, Lee S, Yoon SY, Lee EK, Changd SI, Choo J (2014) SERS-based competitive immunoassay of troponin I and CK-MB markers for early diagnosis of acute myocardial infarction. Chem Commun 50:1033–1148CrossRef
15.
Chon H, Lee S, Yoon SY, Chang SIk, Lima DW, Choo J (2011) Simultaneous immunoassay for the detection of two lung cancer markers using functionalized SERS nanoprobes. Chem Commun 47:12515–12517CrossRef
16.
Yoon J, Choi N, Ko J, Kim Ki, Lee S, Choo J (2013) Highly sensitive detection of thrombin using SERS-based magnetic aptasensors. Biosens Bioelectron 47:62–67CrossRef
17.
Kim I, Junejo IR, Lee Mk, Lee S, Lee EK, Chang SI, Choo J (2012) SERS-based multiple biomarker detection using a gold-patterned microarray chip. J Mol Struct 1023:197–203CrossRef
18.
Mk Lee, Lee S, Lee JH, Limb HW, Seonga GH, Lee EK, Changd SI, Chil HO, Choo J (2011) Highly reproducible immunoassay of cancer markers on a gold-patterned microarray chip using surface-enhanced Raman scattering imaging. Biosens Bioelectron 26:2135–2141CrossRef
19.
Lee MK, Kangsun Lee, Kim KH, Oh KW, Choo J (2012) SERS-based immunoassay using a gold array-embedded gradient microfluidic chip. Lab Chip 12:3720–3727CrossRef
20.
Wang YQ, Yan B, Chen LX (2013) SERS tags: novel optical nanoprobes for bioanalysis. Chem Rev 113:1391–1428CrossRef
21.
Schwartzberg AM, Tammy YO, Talley CE, Zhang JZ (2011) Synthesis, characterization, and tunable optical properties of hollow gold nanospheres. J Phys Chem B 110:19935–19944CrossRef
22.
Newhouse RJ, Wang HN, Hensel JK, Wheeler DA, Zou SL, Zhang JZ (2011) Coherent vibrational oscillations of hollow gold nanospheres. J Phys Chem Lett 2:228–235CrossRef
23.
Xie HN, Larmour IA, Smith WE, Faulds K, Graham D (2012) Surface-enhanced Raman scattering investigation of hollow gold nanospheres. J Phys Chem C 116:8338–8342CrossRef
24.
Xie HN, Larmour IA, Chen YC, Wark AW, Tileli V, McComb DW, Fauldsa K, Graham D (2013) Synthesis and NIR optical properties of hollow gold nanospheres with LSPR greater than one micrometer. Nanoscale 5:765–771CrossRef
25.
Sakao YK, Tomimitsu SJ, Takeda YJ, Natsuaki M, Itoh T (2004) Carcinoembryonic antigen as a predictive factor for postoperative tumor relapse in early-stage lung adenocarcinoma. Eur J Cardiothorac 25:520–522CrossRef
26.
Sawabata N, Ohta M, Takeda S, Hirano H, Okumura Y, Asada H, Maeda H (2002) Serum carcinoembryonic antigen level in surgically resected clinical stage I patients with non-small cell lung cancer. Ann Thorac Surg 74:174–179CrossRef
27.
Altintas Z, Uludaga Y, Gurbuzb Y, Tothill IE (2009) Surface plasmon resonance based immunosensor for the detection of the cancer biomarker carcinoembryonic antigen. Talanta 86:377–383CrossRef
28.
Li H, Ma XY, Dong J, Qian WP (2009) Development of methodology based on the formation process of gold nanoshells for detecting hydrogen peroxide scavenging activity. Anal Chem 81:8916–8922CrossRef
29.
Shao MF, Ning FY, Zhao JW, Wei M, Evans DG, Duan X (2012) Preparation of Fe3O4@SiO2@layered double hydroxide core-shell microspheres for magnetic separation of proteins. J Am Chem Soc 134:1071–1077CrossRef
Metadata
Title
SERS tags-based novel monodispersed hollow gold nanospheres for highly sensitive immunoassay of CEA
Authors
Mingde Guo
Jian Dong
Wei Xie
Lin Tao
Wenbo Lu
Ying Wang
Weiping Qian
Publication date
01-05-2015
Publisher
Springer US
Published in
Journal of Materials Science / Issue 9/2015
Print ISSN: 0022-2461
Electronic ISSN: 1573-4803
DOI
https://doi.org/10.1007/s10853-015-8825-3

Other articles of this Issue 9/2015

Journal of Materials Science 9/2015 Go to the issue

Original Paper

Drastic modification of graphene oxide properties by incorporation of nickel: a simple inorganic chemistry approach

Original Paper

Templated non-hydrolytic synthesis of mesoporous zirconium silicates and their catalytic properties

Original Paper

Underwater discharge plasma-induced coating of poly(acrylic acid) on polypropylene fiber

Review

AlGaN devices and growth of device structures

Original Paper

Germanosilicate glass–ceramics for non-linear optics

Original Paper

Structure and microwave dielectric properties of La5−x Sr x Ti4+x Ga1−x O17 ceramics

Premium Partners

    Image Credits