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Promising Biomarkers: MicroRNAs at Diagnosis, Therapy and Prognostic Evaluation of Breast Cancer Read chapter Read first chapter

2014 | OriginalPaper | Chapter

69. Serum miR-124 and TNF-α are Biomarkers of Ischemic Cerebrovascular Disease

Authors : Jiajie Liu, Xinghua Liao, Nan Wang, Jun Zhou, Lian Duan, Dalin Lu, Zhipeng Liu, Tingbao Yan, Deyun Ma, Xiumei Dong, Xueguang Sun, Tong-Cun Zhang

Published in: Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Publisher: Springer Berlin Heidelberg

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Abstract

MicroRNAs are small non-coding RNAs with 19-22 nucleotides. MiR-124 expresses abundantly in brain tissue and is involved in the development of ischemic cerebrovascular disease (ICVD). Tumor necrosis factor-alpha (TNF-α) is one of the important factors of the inflammatory reaction after cerebral ischemia. To explore the roles of miR-124 and TNF-α in the process of ICVD, here, we collected the serum of 15 patients with ICVD and 5 normal individuals. The levels of miR-124 and TNF-α were detected by real-time PCR and ELISA. The results showed that compared with the normal individuals, the levels of miR-124 and TNF-α in serum were upregulated in the patients, while they were downregulated after clinical treatment, suggesting that these two factors could be used as good biomarkers for the diagnosis and prognosis of ICVD. Besides, our research also provided a theory foundation for understanding the mechanism of inflammatory response in cerebral ischemia mediated by miR-124.

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Metadata
Title
Serum miR-124 and TNF-α are Biomarkers of Ischemic Cerebrovascular Disease
Authors
Jiajie Liu
Xinghua Liao
Nan Wang
Jun Zhou
Lian Duan
Dalin Lu
Zhipeng Liu
Tingbao Yan
Deyun Ma
Xiumei Dong
Xueguang Sun
Tong-Cun Zhang
Copyright Year
2014
Publisher
Springer Berlin Heidelberg
Book
Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Print ISBN: 978-3-642-37921-5

Electronic ISBN: 978-3-642-37922-2

Copyright Year: 2014

DOI
https://doi.org/10.1007/978-3-642-37922-2_69

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