Skip to main content
Disciplines
Automotive Business IT + Informatics Construction + Real Estate Electrical Engineering + Electronics Energy + Sustainability Insurance + Risk Finance + Banking Management + Leadership Marketing + Sales Mechanical Engineering + Materials
Events
DE
EN
Books
Journals
Topic Page
Marketing
Start single access now
Access for companies
EXTENDED SEARCH
Log in
Top
Journal of Nanoparticle Research
Published in: Journal of Nanoparticle Research 11/2014

01-11-2014 | Research Paper

Silica nanoparticles mediated neuronal cell death in corpus striatum of rat brain: implication of mitochondrial, endoplasmic reticulum and oxidative stress

Authors: Arshiya Parveen, Syed Husain Mustafa Rizvi, Farzana Mahdi, Sandeep Tripathi, Iqbal Ahmad, Rajendra K. Shukla, Vinay K. Khanna, Ranjana Singh, Devendra K. Patel, Abbas Ali Mahdi

Published in: Journal of Nanoparticle Research | Issue 11/2014

Log in

Activate our intelligent search to find suitable subject content or patents.

search-config
loading …

Abstract

Extensive uses of silica nanoparticles (SiNPs) in biomedical and industrial fields have increased the risk of exposure, resulting concerns about their safety. We focussed on some of the safety aspects by studying neurobehavioural impairment, oxidative stress (OS), neurochemical and ultrastructural changes in corpus striatum (CS) of male Wistar rats exposed to 80-nm SiNPs. Moreover, its role in inducing mitochondrial and endoplasmic reticulum (ER) stress-mediated neuronal apoptosis was also investigated. The results demonstrated impairment in neurobehavioural indices, and a significant increase in lipid peroxide levels (LPO), hydrogen peroxide (H2O2), superoxide (O2 -) and protein carbonyl content, whereas there was a significant decrease in the activities of the enzymes, manganese superoxide dismutase (Mn SOD), glutathione peroxidase (GPx), catalase (CAT) and reduced glutathione (GSH) content, suggesting impaired antioxidant defence system. Protein (cytochrome c, Bcl-2, Bax, p53, caspase-3, caspase 12 and CHOP/Gadd153) and mRNA (Bcl-2, Bax, p53 and CHOP/Gadd153, cytochrome c) expression studies of mitochondrial and ER stress-related apoptotic factors suggested that both the cell organelles were involved in OS-mediated apoptosis in treated rat brain CS. Moreover, electron microscopic studies clearly showed mitochondrial and ER dysfunction. In conclusion, the result of the study suggested that subchronic SiNPs’ exposure has the potential to alter the behavioural activity and also to bring about changes in biochemical, neurochemical and ultrastructural profiles in CS region of rat brain. Furthermore, we also report SiNPs-induced apoptosis in CS, through mitochondrial and ER stress-mediated signalling.
previous article Evaluation of the efficiency of tumor and tissue delivery of carrier-mediated agents (CMA) and small molecule (SM) agents in mice using a novel pharmacokinetic (PK) metric: relative distribution index over time (RDI-OT)
next article Potential foliar fertilizers with copper and zinc dual micronutrients in nanocrystal suspension

Dont have a licence yet? Then find out more about our products and how to get one now:

Springer Professional "Wirtschaft+Technik"

Online-Abonnement

Mit Springer Professional "Wirtschaft+Technik" erhalten Sie Zugriff auf:

  • über 102.000 Bücher
  • über 537 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Finance + Banking
  • Management + Führung
  • Marketing + Vertrieb
  • Maschinenbau + Werkstoffe
  • Versicherung + Risiko

Jetzt Wissensvorsprung sichern!

inform now

Springer Professional "Technik"

Online-Abonnement

Mit Springer Professional "Technik" erhalten Sie Zugriff auf:

  • über 67.000 Bücher
  • über 390 Zeitschriften

aus folgenden Fachgebieten:

  • Automobil + Motoren
  • Bauwesen + Immobilien
  • Business IT + Informatik
  • Elektrotechnik + Elektronik
  • Energie + Nachhaltigkeit
  • Maschinenbau + Werkstoffe




 

Jetzt Wissensvorsprung sichern!

inform now
Literature
Aebi H (1984) Catalase in vitro. In: Packer L (ed) Methods in enzymology, vol 105. Academic Press, New York, pp 121–126
Barbara SB, Earl RS (1997) Protein oxidation in aging, disease and oxidative stress. J Biol Chem 272:20313–20316CrossRef
Barone MV, Crozat A, Tabaee A, Philipson L, Ron D (1994) CHOP (GADD153) and its oncogenic variant, TLS-CHOP, have opposing effects on the induction of G1/S arrest. Genes Dev 8:453–464CrossRef
Bartczak D, Sanchez-Elsner T, Louafi F, Millar TM, Kanaras AG (2011) Receptor-mediated interactions between colloidal gold nanoparticles and human umbilical vein endothelial cells. Small 7:388–394CrossRef
Bharali DJ, Klejbor I, Stachowiak EK, Dutta P, Roy I, Kaur N, Bergey EJ, Prasad PN, Stachowiak MK (2005) Organically Modified Silica Nanoparticles: a Nonviral Vector for In Vivo Gene Delivery and Expression in the Brain. Proc Natl Acad Sci USA 32:11539–11544CrossRef
Bottini M, D’Annibale F, Magrini A, Cerignoli F, Arimura Y, Dawson MI, Bergamaschi E, Rosato N, Bergamaschi A, Mustelin T (2007) Quantum dot-doped silica nanoparticles as probes for targeting of T-lymphocytes. Int J Nanomed 2:227–233
Brouwer D (2010) Exposure to manufactured nanoparticles in different workplaces. Toxicology 269:120–127CrossRef
Bugiani O, Perdelli F, Salvarani S, Leonardi A, Mancardi GL (1980) Loss of striatal neurons in Parkinson’s disease: a cytometric study. Eur Neurol 19:339–344CrossRef
Chen G, Ma C, Bower KA, Shi X, Ke Z, Luo J (2008) Ethanol promotes endoplasmic reticulum stress-induced neuronal death: involvement of oxidative stress. J Neurosci Res 86:937–946CrossRef
Christen V V, Capelle M, Fent K (2013) Silver nanoparticles induce endoplasmatic reticulum stress response in zebrafish. Toxicol Appl Pharmacol 272:519–528CrossRef
Circu ML, Aw TY (2010) Reacting oxygen species, cellular redox system and apoptosis. Free Radical Biol Med 6:749–762CrossRef
Deniaud A, Sharaf el dein O, Maillier E, Poncet D, Kroemer G, Lemaire C, Brenner C (2008) Endoplasmic reticulum stress induces calcium-dependent permeability transition, mitochondrial outer membrane permeabilization and apoptosis. Oncogene 27:285–299CrossRef
Doyle KM, Kennedy D, Gorman AM, Gupta S, Healy SJ, Samali A (2011) Unfolded proteins and endoplasmic reticulum stress in neurodegenerative disorders. J Cell Mol Med 15:2025–2039CrossRef
Elder A, Gelein R, Silva V, Feikert T, Opanashuk L, Carter J, Potter R, Maynard A, Ito Y, Finkelstein J, Oberdörster G (2006) Translocation of inhaled ultrafine manganese oxide particles to the central nervous system. Environ Health Perspect 114:1172–1178CrossRef
Flohe L, Gunzler WA (1984) Assays of glutathione peroxidase. Method Enzymol 105:114–121CrossRef
Galehdar Z, Swan P, Fuerth B, Callaghan SM, Park DS, Cregan SP (2010) Neuronal apoptosis induced by endoplasmic reticulum stress is regulated by ATF4-CHOP-mediated induction of the Bcl-2 homology 3-only member PUMA. J Neurosci 30:16938–16948CrossRef
Glowinski J, Iversen LL (1966) Regional studies of catecholamines in the rat brain. The disposition of 3H-norepinephrine 3H-dopamine and 3H-dopa in various regions of the brain. J Neurochem 13:655–659CrossRef
Graham DG (1978) Oxidative pathways for catecholamines in the genesis of neuromelanin and cytotoxic quinones. Mol Pharmacol 14:633–643
Hasan M, Haider SS (1989) Acetyl homocysteine thiolactone protect against some neurotoxic effects of thallium. Neurotoxicology 10:257–262
Hu R, Zheng L, Zhang T, Gao G, Cui Y, Cheng Z, Cheng J, Hong M, Tang M, Hong F (2011) Molecular mechanism of hippocampal apoptosis of mice following exposure to titanium dioxide nanoparticles. J Hazard Mater 191:32–40CrossRef
Jiang J, Oberdorster G, Bisw P (2009) Characterization of size, surface charge, and agglomeration state of nanoparticle dispersions for toxicological studies. J Nanopart Res 11:77–89CrossRef
Kakkar P, Das B, Viswanathan PN (1984) A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21:130–132
Khanna VK, Husain R, Seth PK (1994) Effect of protein malnutrition on the neurobehavioral toxicity of styrene in young rats. J Appl Toxicol 14:351–356CrossRef
Kim C, Speisky MB, Klarevla SN (1987) Rapid and sensitive method for measuring norepinephrine, dopamine, 5HT and their major metabolites in rat brain by high performance liquid chromatography. J Chromatogr 386:25–35CrossRef
Kroemer G, Galluzzi L, Brenner C (2007) Mitochondrial membrane permeabilization in cell death. Physiol Rev 87:99–163CrossRef
Levine RL, Garland D, Oliver CN, Amici A, Climent I, Lenz AG, Ahn BW, Shaltiel S, Stadtman ER (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol 186:464–478CrossRef
Li G, Scull C, Ozcan L, Tabas I (2010) NADPH oxidase links endoplasmic reticulum stress, oxidative stress, and PKR activation to induce apoptosis. J Cell Biol 191:1113–1125CrossRef
Liao CM, Chiang YH, Chio CP (2009) Assessing the airborne titanium dioxide nanoparticles related exposure hazard at workplace. J Hazard Mater 162:57–65CrossRef
Liu X, Sun J (2010) endothelial cells dysfunction induced by silica nanoparticles through oxidative stress via JNK/P53 and NF-kappaB pathways. Biomaterials 32:8198–8209CrossRef
Liu S, Xu L, Zhang T, Ren G, Yang Z (2010) Oxidative stress and apoptosis induced by nanosized titanium dioxide in PC12 cells. Toxicology 267:172–177CrossRef
Lockman PR, Koziara JM, Mumper RJ, Allen DD (2004) Nanoparticle surface charges alter blood–brain barrier integrity and permeability. J Drug Target 12:635–641CrossRef
Lowry OH, Rosenbrough NJ, Farr AL Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 192:265–275
Matsumoto M, Minami M, Takeda K, Sakao Y, Akira S (1996) Ectopic expression of CHOP (GADD153) induces apoptosis in M1 myeloblastic leukemia cells. FEBS Lett 395:143–147CrossRef
Meiser J, Weindl D, Hiller K (2013) Complexity of dopamine metabolism. Cell Commun Signal 11:34CrossRef
Momoi T (2004) Caspases involved in ER stress-mediated cell death. J Chem Neuroanat 28:101–105CrossRef
Murdock RC, Braydich-Stolle L, Schrand AM, Schlager JJ, Hussain SM (2008) Characterization of nanomaterial dispersion in solution prior to in vitro exposure using dynamic light scattering technique. Toxicol Sci 101:239–253CrossRef
Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J (2000) Caspase-12 mediates endoplasmic reticulum-specific apoptosis and cytotoxicity by amyloid-β. Nature 403:98–103CrossRef
Napolitano A, Manini P, D’Ischia M (2011) Oxidation chemistry of catecholamines and neuronal degeneration: an update. Curr Med Chem 18:1832–1845CrossRef
Nourooz-Zadeh J, Tajaddini-Sarmadi T, Wolff SP (1994) Measurement of plasma hydroperoxide concentrations by the ferrous oxidation-xylenol orange assay in conjunction with triphenylphosphine. Anal Biochem 220:403–409CrossRef
Nozaki S, Sledge GW Jr, Nakshatri H (2001) Repression of GADD153/CHOP by NF-kappaB: a possible cellular defense against endoplasmic reticulum stress-induced cell death. Oncogene 20:2178–2185CrossRef
Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358CrossRef
Oliveira CP, Lopasso FP, Laurindo FR (2001) Protection against liver ischemia–reperfusion injury in rats by silymarin or verapamil. Transplant. Proc 33:3010–3014CrossRef
Oszlanczi G, Papp A, Szabo A (2011) Nervous system effects in rats on subacute exposure by lead-containing nanoparticles via the airways. Inhal Toxicol 23:173–181CrossRef
Parveen A, Rizvi SHM, Gupta A, Singh R, Ahmad I, Mahdi F, Mahdi AA, Singh R, Ahmad I, Mahdi F, Mahdi AA (2012) NMR-based metabonomics study of sub-acute hepatotoxicity induced by silica nanoparticles in rats after intranasal exposure. NMR Cell Mol Biol 58(Supp):196–203
Pitcher TL, Melzer TR, Macaskill MR, Graham CF, Livingston L, Keenan RJ, Watts R, Dalrymple-Alford JC, Anderson TJ (2012) Reduced striatal volumes in Parkinson’s disease: a magnetic resonance imaging study. Transl Neurodegener. 21:17CrossRef
Rizvi SHM, Parveen A, Verma AK, Ahmad I, Arshad M, Mahdi AA (2014) Aluminium induced endoplasmic reticulum stress mediated cell death in SHSY5Y neuroblastoma cell line is independent of p53. PLoS ONE 9(5):e98409CrossRef
Sanges D, Marigo V (2006) Cross-talk between two apoptotic pathways activated by endoplasmic reticulum stress: differential contribution of caspase-12 and AIF. Apoptosis 11:1629–1641CrossRef
Shafer TJ, Meyer DA, Crofton KM (2005) Developmental neurotoxicity of pyrethroid insecticides: critical review and future research needs. Environ Health Perspect 113:123–136CrossRef
Slowing II, Trewyn BG, Lin VSY (2007) Mesoporous silica nanoparticles for intracellular delivery of membrane-impermeable proteins. J Am Chem Soc 129:8845–8849CrossRef
Sterling NW, Du G, Lewis MM, Dimaio C, Kong L, Eslinger PJ, Styner M, Huang X (2013) Striatal shape in Parkinson’s disease. Neurobiol Aging 34:2510–2516CrossRef
Sulzer D, Bogulavsky J, Larsen KE, Behr G, Karatekin E, Kleinman MH, Turro N, Krantz D, Edwards RH, Greene LA, Zecca L (2000) Neuromelanin biosynthesis is driven by excess cytosolic catecholamines not accumulated by synaptic vesicles. Proc Natl Acad Sci USA 97:11869–11874CrossRef
Takenaka S, Karg E, Roth C, Schulz H, Ziesenis A, Heinzmann U, Schramel P, Heyder J (2001) Pulmonary and systemic distribution of inhaled ultrafine silver particles in rats. Environ Health Perspect 109:547–551CrossRef
Tang DG, Li L, Zhu Z, Joshi B (1998) Apoptosis in the absence of cytochrome c accumulation in the cytosol. Biochem. Biophys Res 242:380–384CrossRef
Vacha R, Martinez-Veracoechea FJ, Frenkel D (2012) Intracellular release of endocytosed nanoparticles upon a change of ligand–receptor interaction. ACS Nano 6:10598–10605
Walter L, Hajnoczky G (2005) Mitochondria and endoplasmic reticulum: the lethal interorganelle cross-talk. J Bioenerg Biomembr 37:191–206CrossRef
Wang J, Liu Y, Jiao F, Lao F, Li W, Gu Y, Li Y, Ge C, Zhou G, Li B, Zhao Y, Chai Z, Chen C (2008) Time-dependent translocation and potential impairment on central nervous system by intranasally instilled TiO2 nanoparticles. Toxicology 254:82–90CrossRef
Wang B, Feng WY, Zhu MT, Wang Y, Wang M, Gu Y, Ouyang H, Wang H, Li M, Zhao Y, Chai Z, Wang H (2009a) Neurotoxicity of low-dose repeatedly intranasal instillation of nano- and submicron-sized ferric oxide particles in mice. J Nanopart Res 11:41–53CrossRef
Wang J, Rahman MF, Duhart HM, Newport GD, Patterson TA, Murdock RC, Hussain SM, Schlager JJ, Ali SF (2009b) Expression changes of dopaminergic system-related genes in PC12 cells induced by manganese, silver, or copper nanoparticles. Neurotoxicology 30:926–933CrossRef
Win Shwe TT, Yamamoto S, Ahmed S, Kakeyama M, Kobayashi T, Fujimaki H (2006) Brain cytokine and chemokine mRNA expression in mice induced by intranasal instillation with ultrafine carbon black. Toxicol Lett 163:153–160CrossRef
Wu HC, Yang CY, Hung DZ, Su CC, Chen KL, Yen CC, Ho TJ, Su YC, Huang CF, Chen CH, Tasi LM, Chen YW (2011a) Nickel (II) induced JNK activation regulated mitochondria dependent apoptosis pathway leading to cultured rat pancreatic β-cell death. Toxicology 289:103–111CrossRef
Wu J, Wang C, Sun J, Xue Y (2011b) Neurotoxicity of silica nanoparticles: brain localization and dopaminergic neurons damage pathways. ACS Nano 5:4476–4489CrossRef
Yadav RS, Sankhwar ML, Shukla Rk, Chandra R, Pant AB, Islam F, Khanna VK (2009) Attenuation of arsenic neurotoxicity by curcumin in rats. Toxicol Appl Pharmacol 240:367–376CrossRef
Zhang R, Piao JM, Kim KC, Kim AD, Choi JY, Choi J, Hyun JW (2012) Endoplasmic reticulum stress signaling is involved in silver nanoparticles-induced apoptosis. Int J Biochem Cell Biol 44:224–232CrossRef
Metadata
Title
Silica nanoparticles mediated neuronal cell death in corpus striatum of rat brain: implication of mitochondrial, endoplasmic reticulum and oxidative stress
Authors
Arshiya Parveen
Syed Husain Mustafa Rizvi
Farzana Mahdi
Sandeep Tripathi
Iqbal Ahmad
Rajendra K. Shukla
Vinay K. Khanna
Ranjana Singh
Devendra K. Patel
Abbas Ali Mahdi
Publication date
01-11-2014
Publisher
Springer Netherlands
Published in
Journal of Nanoparticle Research / Issue 11/2014
Print ISSN: 1388-0764
Electronic ISSN: 1572-896X
DOI
https://doi.org/10.1007/s11051-014-2664-z

Other articles of this Issue 11/2014

Journal of Nanoparticle Research 11/2014 Go to the issue

Perspectives

Free nanoparticle characterization by optical scattered field analysis: opportunities and perspectives

Research Paper

Nanostructured microspheres of silver @ zinc oxide: an excellent impeder of bacterial growth and biofilm

Research Paper

Paclitaxel-loaded KMnF3 nanoparticles for cancer imaging and therapy in vivo

Research Paper

Gold nanoparticles induce DNA damage in the blood and liver of rats

Research Paper

Experimental study of fractal clusters formation from nanoparticles synthesized by atmospheric pressure plasma-enhanced chemical vapor deposition

Research Paper

Gold nanocage coupled single crystal TiO2 nanostructures for near-infrared water photolysis

Premium Partners

    Image Credits