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2019 | OriginalPaper | Chapter

3. Single-Cell Culture and Analysis on Microfluidics

Authors : Weiwei Li, Jin-Ming Lin

Published in: Microfluidics for Single-Cell Analysis

Publisher: Springer Singapore

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Abstract

Heterogeneity of cell populations is a major obstacle for understanding complex biological processes. In order to have a more comprehensive quantitative comprehending of cellular processes, it is necessary to quantify the distribution of behavior in a population of individual cells. Analysis of single-cell behaviors requires efficient single-cell capture, controllable single-cell culture performance as well as reliable analysis techniques. The microfluidic system provides advanced technology for single-cell culture and observation. This chapter gives a brief account of single-cell capture by microfluidic methods, long-term single-cell culture on both two-dimensional models and three-dimensional microfluidic systems, as well as single-cell growth and differentiation in a microfluidic environment. Furthermore, the advanced methods used for characterizing on-chip single-cell culture were also discussed.

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Title: Single-Cell Culture and Analysis on Microfluidics
Authors: Weiwei Li
Jin-Ming Lin
Publisher: Springer Singapore
Book: Microfluidics for Single-Cell Analysis
Print ISBN: 978-981-329-728-9

Electronic ISBN: 978-981-329-729-6

Copyright Year: 2019
DOI: https://doi.org/10.1007/978-981-32-9729-6_3