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2019 | OriginalPaper | Buchkapitel

3. Single-Cell Culture and Analysis on Microfluidics

verfasst von : Weiwei Li, Jin-Ming Lin

Erschienen in: Microfluidics for Single-Cell Analysis

Verlag: Springer Singapore

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Abstract

Heterogeneity of cell populations is a major obstacle for understanding complex biological processes. In order to have a more comprehensive quantitative comprehending of cellular processes, it is necessary to quantify the distribution of behavior in a population of individual cells. Analysis of single-cell behaviors requires efficient single-cell capture, controllable single-cell culture performance as well as reliable analysis techniques. The microfluidic system provides advanced technology for single-cell culture and observation. This chapter gives a brief account of single-cell capture by microfluidic methods, long-term single-cell culture on both two-dimensional models and three-dimensional microfluidic systems, as well as single-cell growth and differentiation in a microfluidic environment. Furthermore, the advanced methods used for characterizing on-chip single-cell culture were also discussed.

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Vorheriges Kapitel Microfluidic Technology for Single-Cell Capture and Isolation

Nächstes Kapitel Microfluidic Technology for Single-Cell Manipulation

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Titel: Single-Cell Culture and Analysis on Microfluidics
verfasst von: Weiwei Li
Jin-Ming Lin
Verlag: Springer Singapore
Buch: Microfluidics for Single-Cell Analysis
Print ISBN: 978-981-329-728-9

Electronic ISBN: 978-981-329-729-6

Copyright-Jahr: 2019
DOI: https://doi.org/10.1007/978-981-32-9729-6_3

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