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Journal of Sol-Gel Science and Technology

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01-09-2013 | Original Paper

Sol–gel immobilized enzymatic glucose biosensor on gold interdigitated array (IDA) microelectrode

Authors: Hao-Min Huang, Pin-Kang Huang, Wei-Hsuan Kuo, Yi-Hsu Ju, Meng-Jiy Wang

Published in: Journal of Sol-Gel Science and Technology | Issue 3/2013

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Abstract

An enzymatic glucose biosensor with good sensitivity, selectivity and stability employing interdigitated array microelectrode (IDA μ-electrode) was reported. IDA μ-electrode was prepared by photolithography method with its surface immobilized with a layer of glucose oxidase (GOx), entrapped in a three-dimensional network composed of chitosan and tetraethyl orthosilicate sol–gel. The surface of the as-prepared IDA μ-electrode was characterized by scanning electron microscope, electron spectroscopy for chemical analysis, and atomic force microscopy. The experimental parameters for the best glucose sensing performance were optimized according to the loading of GOx, the applied voltages, the concentration of mediator, and the pH for glucose detection. The resulted biosensor exhibited a good response to glucose with a wide linear range from 0 to 35 mM and a low detection limit of 1 mM. The glucose sensor also showed a short response time (within 5 s) that the fast response was reflected by the small Michaelis–Menten constant (K_M ^app) with a value of 2.94 mM. The reported glucose biosensor exhibited good sensitivity (8.74 μA/mM.cm²), reproducibility, and stability.

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Title: Sol–gel immobilized enzymatic glucose biosensor on gold interdigitated array (IDA) microelectrode
Authors: Hao-Min Huang
Pin-Kang Huang
Wei-Hsuan Kuo
Yi-Hsu Ju
Meng-Jiy Wang
Publication date: 01-09-2013
Publisher: Springer US
Published in: Journal of Sol-Gel Science and Technology / Issue 3/2013
Print ISSN: 0928-0707
Electronic ISSN: 1573-4846
DOI: https://doi.org/10.1007/s10971-013-3106-6

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