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11-04-2020 | Note

Specimen preparation optimization for size and morphology characterization of nanocellulose by TEM

Authors: Laura C. E. da Silva, Alexandre Cassago, Liliane C. Battirola, Maria do Carmo Gonçalves, Rodrigo V. Portugal

Published in: Cellulose | Issue 9/2020

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Abstract

Nanoparticle morphology, size and dispersion are key parameters for the application of cellulose nanomaterials in various areas, such as polymer nanocomposites, catalysts, gel and so on. Transmission electron microscopy (TEM) is the most suitable technique for the morphological characterization of these particles. However, nanocellulose low contrast in TEM images is the major drawback for their adequate morphological characterization and size determination. Even though it is widespread knowledge that negative staining using uranyl acetate is the best approach for intensifying cellulose contrast, up to now few have succeeded in achieving high quality images and reliable size measurements of these nanomaterials. This protocol presents an optimization of the standard uranyl acetate protocol commonly used for biological specimens in order to suit cellulose nanomaterials. Drying method and grid conditions were proven to be the most significant variables for effective TEM specimen preparation. These guidelines could also be successfully applied to enhance the cellulose nanomaterial contrast in polymer matrices.

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Title: Specimen preparation optimization for size and morphology characterization of nanocellulose by TEM
Authors: Laura C. E. da Silva
Alexandre Cassago
Liliane C. Battirola
Maria do Carmo Gonçalves
Rodrigo V. Portugal
Publication date: 11-04-2020
Publisher: Springer Netherlands
Published in: Cellulose / Issue 9/2020
Print ISSN: 0969-0239
Electronic ISSN: 1572-882X
DOI: https://doi.org/10.1007/s10570-020-03116-7

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